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Biomedical subjects

E Huber

Publications and source records attributed to E Huber.

At least 19 recordsLinked to original sources
PubMed1992

[Movement therapy. With special reference to functional disorders of the locomotor system].

Physical therapy by movements is of importance in several medical fields, in particular in rehabilitation. A great variety of possibilities for treatment by exercises and manual therapy exists. In a first part we outline a useful and problem-oriented nomenclature derived from actual applications of physical therapy by movements based on various concepts. The bases and applications of functional therapy by movements, by training or manipulative procedures as well as other therapeutic means are presented. The therapeutic climate and the relation between doctor and physiotherapist are briefly explored. In a second part we present the most important traditional concepts of treatment by movements in a more detailed way.

Exercise Therapy↗
PubMed1992

Acquired dacryocystitis: microbiology and conservative therapy.

The dacryocystitis in adults is mainly caused by postsaccal stenosis of the lacrimal ducts. The banking up of the lacrimal fluid leads to an accumulation of germs and following infection. This report describes the clinical and microbiological findings in a large consecutive series of patients that presented at the outpatient clinic of the 2nd Department of Ophthalmology of the University of Vienna with the signs of acute, chronic recurrent or chronic infections of the lacrimal system between 1983 and the end of 1990. Within the bacterial genus Staphylococci (S. aureus, S. epidermidis and S. saprophyticus) were the most frequently isolated organisms (74 cultures = 50% out of samples with positive cultures). It was quite interesting that a significant number of gram-negative rods (37 = 25.5%) could be isolated. Of these microorganisms Escherichia coli was most frequently growing on special media (17 cases = 11.7%) when cultures were obtained from acute inflamed lacrimal sacs of patients who suffered from chronic recurrent infections. Conservative therapy of purulent dacryocystitis constitutes the last possible preparation for a necessary surgical intervention and therefore the authors want to point out the importance of microbiological examinations so as to optimize antibiotic therapy.

Acute Disease↗
PubMed1992

[Documentation of digital dacryocystography using video technique].

Digital dacryocystography is considered the optimal radiodiagnostic method in obstructions of the lacrimal pathways. The examination is carried out by a computer-controlled X-ray unit with a C-arc attached to an image-intensifying TV system. In this way the advantages of this technique and of modern video recording are linked. The bilateral filling process of the contrast medium is recorded and the findings simultaneously recorded on videotape. Consequently, pictures of the dynamic flow are retrievable at any time. This provides good diagnostic documentation and an outstanding basis for any scientific analysis.

Diagnosis, Differential↗
PubMed1992

[Patent ductus arteriosus in a dog: modified method of double ligation].

Patent ductus arteriosus, a common congenital cardio-vascular disease, needs surgery for correction. Three methods are described: 1. transsection and suturing of the ductus ends; 2. dissection of the ductus itself and occlusion by double ligation (classical method), 3. modified double ligation described by Jackson and Henderson. A modification of the technique is described, based on the previous methods of double ligation.

Animals↗
PubMed1991

Videodocumentation in digital dacryocystography.

The digital dacryocystography proved to be the optimum method of X-ray diagnostics in obstructions of the lacrimal pathways. The examination was performed with a computer controlled X-ray unit with a C-arc coupled to an image intensifier tv-system. In the study the advantages of this technique were combined with the advantages of modern videotechnique. A nonionic water soluble contrast medium was used. The bilateral contrast medium filling process was registrated and the findings were recorded on videotape concurrently. The videocassettes, marked with the data of the patients and the date of examination, are stored in a video-tape library. This provides an always repeatable diagnostic documentation and it is also an excellent base for scientific analyses. The technique and the results of the examinations, performed with 18 patients, are described.

Adult↗
PubMed1991

[Capsular sack implantation of heparin-modified posterior chamber lenses].

In a prospective study, heparin surface modified posterior chamber lenses were implanted into the capsular bag in 50 patients. The exact positioning of the biconvex, one-piece lens in the bag is the prerequisite for good centration and for an optimal result. The hydrophilisation of the originally hydrophobic PMMA material is achieved by heparin surface modification and results in less inflammation. The aim of this study was to investigate the postoperative behavior of the HSM posterior chamber lens implanted in the bag, with special consideration given to intraocular inflammation. In the postoperative follow-up time of one to seven months, hundred percent of the patients with physiological fundus reached a postoperative visual acuity of 0.7 or better.

Aged↗
PubMed1990

Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2'-deoxyuridine-5'-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin).

The chemical syntheses of novel digoxigenin-derivatized compounds are described which are modified substrates for enzymatically or photochemically non-radioactive digoxigenin labeling of nucleic acids. Various activated digoxigenin-haptens are coupled to 5-aminoallyl-substituted 2'-deoxyuridine-5'-triphosphate. This results in digoxigenin-modified nucleoside triphosphates of variable spacer lengths (Dig-[4]-dUTP/Dig-[11]-dUTP/Dig-[16]-dUTP) which can be used as substrates for enzymatic labeling of DNA with digoxigenin-haptens by Klenow enzyme-catalysed random-primed synthesis. In addition the synthesis of N-[4-azidobenzoyl]-N'-[(3-O-digoxigeninyl)methylcarbonyl)]-1 ,8-diamino- 3,6-dioxaoctane (photodigoxigenin), a photoactivatable analog of digoxigenin, is described which can be applied for photolabeling of DNA and RNA with digoxigenin-haptens leaving the nucleic acid molecules intact.

DNA↗
PubMed1978

Determination of microsomal UDP-glucuronyltransferase in needle-biopsy specimens of human liver.

Sensitive, reliable and convenient assays are described for the study of human liver microsomal UDP-glucuronyltransferase. Using 14C-1-naphthol as substrate about 2 mg of a liver biopsy specimen and for 14C-morphine about 20 mg of tissue will suffice for enzyme estimation. Lack of inhibition of 1-naphthol glucuronidation by morphine suggests that the substrates are glucuronidated by different forms of the enzyme. Enzyme levels in the native and activated state were studied in biopsies from patients grouped according to histopathological and clinical criteria. The enzyme assays may help to characterize UDP-glucuronyltransferases in human tissues and their induction by drugs and environmental chemicals, as well as their alteration in various diseases.

Animals↗
PubMed1978

A characterization of the nucleotide uptake of chromaffin granules of bovine adrenal medulla.

Chromaffin granules isolated from bovine adrenal gland were incubated with (3)H-labelled nucleotides and [(14)C]noradrenaline to study the uptake of these substances. [(3)H]ATP, [(3)H]ADP and [(3)H]AMP are taken up by these organelles by the same temperature-dependent mechanism. The apparent K(m) for ATP and ADP is 1.4mm, and for AMP it is 2.9mm. The uptake of ATP has a flat pH optimum, whereas the catecholamine uptake increases with more alkaline pH. Atractyloside and carboxyatractyloside are competitive and specific inhibitors of nucleotide uptake, whereas reserpine inhibits only that for catecholamines. Mg(2+) ions activate uptake of both catecholamine and nucleotides, whereas EDTA and N-ethylmaleimide inhibit these processes. Nucleotide and catecholamine uptakes are inhibited by uncouplers of oxidative phosphorylation and by two ATP analogues. NH(4) (+) ions and nigericin in the presence of KCl inhibit only catecholamine uptake. It is concluded that nucleotide uptake, as proposed previously for catecholamine uptake, depends on an electrochemical proton gradient produced by a proton-translocating adenosine triphosphatase localized in the membrane of chromaffin granules. Furthermore, as suggested by the effect of NH(4) (+) and nigericin, catecholamine uptake apparently depends on the chemical part of this gradient, whereas the results for nucleotide uptake are consistent with its dependence on the electrical component.

Adenosine Diphosphate↗
PubMed1977

UDP-glucuronyltransferase in perfused rat liver and in microsomes. Effects of CCl4 injury.

To elucidate the disparity between glucuronidation rates in vivo and UDP-glucuronyltransferase in vitro after CCl4 injury, the time course of the effects of CCl4 (0.25 ml/kg) on kinetic properties of UDP-glucuronyltransferase (l-naphthol as substrate) was examined in rat liver homogenates and microsomes. These experiments were compared with l-naphthol glucuronidation by the perfused liver which was studied at various time points after CCl4 administration. Phenobarbital-treated rats were used to enhance the hepatotoxicity of CCl4. 1. Within 24 h UDP-glucuronyltransferase activity increased 8-fold in liver homogenates and 3-fold in microsomes. During this time the allosteric activator, UDP-N-acetylglucosamine, lost its effect whereas the inhibitor UPD showed greater inhibitory properties, thus counteracting the activation. 2. l-Naphthol glucuronidation in perfused livers was significantly decreased by 24 h. Sulfate ester formation was little affected. 3. The content of UDP-glucuronic acid was not significantly altered although liver histology revealed about 45% necrotic and prenecrotic cells and an uniform fatty degeneration of hepatocytes after 24 h. The results suggest that during CCl4 injury, UDP-glucuronyltransferase is activated. At the same time the kinetic properties of the enzyme are altered in a way leading to inefficient glucuronide synthesis, when assays are carried out under conditions presumed to exist in vivo. Nevertheless the capacity to form glucuronides is retained in the acutely injured liver.

Animals↗