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Biomedical subjects

E J Bell

Publications and source records attributed to E J Bell.

At least 73 records · Page 4Linked to original sources
PubMed1972

Murine typhus toxin: studies on identification of the neutralizing factor present in normal human serum.

Studies were made on the isolation and identification of the Rickettsia typhi toxin-neutralizing factor (TNF) previously demonstrated in normal human serum. By means of various methods of separating serum proteins, such as filtration on Sephadex G-200, dextran precipitation, hydroxyapatite chromatography, and ultracentrifugation, TNF was found to be closely associated with purified serum beta-lipoprotein, although no serological relationship with this protein was demonstrated. Lipase as well as trypsin digestion of purified preparations of beta-lipoprotein destroyed the TN activity. No evidence was obtained for an association of TNF with the immunoglobulins or with any serum protein other than beta-lipoprotein. Further studies revealed that (i) serum specimens with TN titers of 1:1024 and others with titers of 1:8 or less contained the same concentration of beta-lipoprotein; (ii) purified preparations of beta-lipoprotein isolated from TNF positive and negative sera, and which had the same protein concentration, differed as much as 250-fold in TN titer; and (iii) the TN activity of a serum could be removed by absorption with antiserum to beta-lipoprotein from a positive donor, but not with antiserum to beta-lipoprotein from a negative donor.

Agglutination Tests↗
PubMed1972

A micrometabolic inhibition test for the estimation of poliovirus neutralizing antibodies.

A modified micrometabolic inhibition test for the titration of poliovirus neutralizing antibodies is described. The effects of varying both the duration and the temperature of incubation of the serum-virus mixtures were examined. Overnight incubation resulted in a 2-3-fold increase in the titre of the hyperimmune sera tested. The method is simple and economical and appears to be suitable for antibody surveys.

Antibodies, Viral↗
PubMed1971

Laminectomy.

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Laminectomy↗