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Biomedical subjects

E J Perea

Publications and source records attributed to E J Perea.

At least 145 records · Page 8Linked to original sources
PubMed1980

Comparative in vitro activity of 1-oxa-beta-lactam (LY127935) and cefoperazone with other beta-lactam antibiotics against anaerobic bacteria.

The in vitro activity of 1-oxa-beta-lactam (LY127935), cefoperazone (T-1551), cefuroxime, cefsulodin, cefaclor, cefotaxime, and cefoxitin on 85 anaerobic clinical isolates (30 Bacteroides, 30 Clostridium, 25 Peptococcaceae) was simultaneously determined by the agar dilution test in two different media, Brucella Agar (Difco Laboratories) and Wilkins-Chalgren agar. In Wilkins-Chalgren agar, 90% of Bacteroides were inhibited by (micrograms per milliliter): LY127935, 0.5; T-1551, 64; cefoxitin or cefuroxime, 8; cefsulodin or cefotaxime, 32; and cefaclor, 128. All Clostridia were inhibited in Wilkins-Chalgren by (micrograms per milliliter): LY127935, 4; T-1551, 2; cefoxitin, 6; cefuroxime, 0.12; cefsulodin, 0.5; cefaclor, 1; and cefotaxime, 8. All Peptococccaceae were inhibited by T-1551, cefsulodin or cefotaxime at 4 microgram/ml and by cefoxitin or cefuroxime at 1 to 2 microgram/ml. With cefaclor at 8 microgram/ml, 92% of strains were inhibited, and LY127935 at 16 microgram/ml only inhibited 64% of strains. LY127935 was the most active of the antibiotics tested against Bacteroides, showing good activity against Clostridia and poor activity on Peptococcaceae, whereas T-1551 was more active against Peptococccaceae and had similar activity against Clostridia and poor activity on Bacteroides. There are no significant differences between minimal inhibitory concentrations obtained in Brucella Agar and those obtained in Wilkins-Chalgren.

Anaerobiosis↗
PubMed1980

In vitro evaluation of new penicillins and cephalosporins upon P. aeruginosa and their interaction with mecillinam.

The in vitro activity of the new semisynthetic ureidopenicillins azlocillin (AZ) and mezlocillin (MZ), and of the new cephalosporin cefsulodin (CEF) were determined against 50 carbenicillin-sensitive (CARs) and 50 carbenicillin-resistant (CARr) P. aeruginosa clinical isolates. In the CARs group the most active antibiotics are AZ and CEF with MICs between 0.5 and 8 microgram/ml. Ticarcillin (TIC) and MZ showed more activity than CAR with MICs from 2 to 32 mu/ml. In this group there is a predominance of pyocine-type groups 1 and 3. In the CARr group, AZ is the most active antibiotic at low concentrations. At 64 microgram/ml of CEF, 72% of strains are inhibited as compared to 70% with AZ, 62% with MZ and 50% with TIC. In this group there is predominance of non-typeable strains. Interaction with mecillinam (MEC) and these antibiotics was studied on three different culture media (MH, NIH and DST). There were few cases of synergism with MEC and TIC combinations, mostly on DST medium. No appreciable synergism was found with other combinations of antibiotics.

Amdinocillin↗
PubMed1979

Sisomicin in surgical infections. Laboratory and clinical studies.

A clinical and bacteriological study to evaluate the effectiveness and toxicity of sisomicin in the treatment of surgical infections is described. Fifteen patients received i.m. injections of 150 or 225 mg/day for seven days. The daily doses was divided into three applications. The mean serum levels at the first hour were 5.26 +/- 2.41 microgram/ml and at the seventh hour 1.0 +/- 1.19 microgram/ml. Similar values were determined on the seventh day of treatment with sisomicin: 6.36 +/- 2.36 microgram/ml one hour, 1.17 +/- 1.67 microgram/ml seven hours after i.m. injection. All the microorganisms isolated had MICs below the sisomicin levels measured. The clinical results were assessed as "excellent" in 11 patients. Therapeutic results were considered as "fair" in three patients. The clinical conditions of one patient affected by osteomyelitis following osteosynthesis of the femur was not improved by treatment. No side effects were observed.

Adolescent↗
PubMed1978

Synergism of fosfomycin-ampicillin and fosfomycin-chloramphenicol against Salmonella and Shigella.

Ninety strains of Salmonella and 50 strains of Shigella were tested for susceptibility to fosfomycin, chloramphenicol, and ampicillin by the agar dilution method. Drug interaction between fosfomycin-ampicillin and fosfomycin-chloramphenicol was studied by the agar dilution method. The fractional inhibitory concentration was calculated. The combination of fosfomycin-ampicillin was synergistic against Salmonella in 74 cases, additive in 7, indifferent in 7, antagonistic in none, and nonevaluable in 2; against Shigella it was synergistic in 27 cases, additive in 9, indifferent in 14, and antagonistic in none. The combination of fosfomycin-chloramphenicol was synergistic against Salmonella in 56 cases, additive in 9, indifferent in 13, nonevaluable in 12, and antagonistic in none; against Shigella it was synergistic in 29 cases, additive in 10, indifferent in 9, nonevaluable in 2, and antagonistic in none. Killing curves with combinations of each antimicrobial agent showed that the cultures that had proven to be indifferent by the agar dilution method showed a bactericidal effect until h 4, with posterior regrowth of the culture after this time period. For the strains in which synergism was demonstrated, total bactericidal activity was reached at 24 h.

Ampicillin↗
PubMed1977

Genetic localization of the resistance to fosfomycin.

The possible existence of a transferable resistance to fosfomycin was studied in 50 strains of enterobacteria that were isolated in our hospital and which showed a resistance to fosfomycin of more than 256 mug/ml. E. coli K12 E711 was used as a receptor strain in the conjugation -- the times of conjugation were 20 min and 18 h. We found a transferable resistance in 28% of the strains. In all but one of the cases, only the resistance to fosfomycin was transferred. The recombinant which received the resistance to fosfomycin together with three other resistances was used as the donor for the transduction with the phage P1Kc to the receptor strain which was E. coli UTH 1038. A study was carried out on the influence of the radiation of the transducing phages with ultraviolet light on the frequency of transduction. The findings demonstrated a linear decrease in the frequency in proportion to the increase in the time of radiation.

Ampicillin↗
PubMed1975

Mechanical ventilators as vehicles of infection.

Twenty-five patients from the intensive care unit who had been subjected to tracheostomy and require mechanical respiration were studied. Data are presented concerning the bacteriological controls performed on the tracheal exudates, various parts of the ventilators (Engström Model 200 and Bennett PR-2), and the Wright spirometer. The germs found were mainly pseudomonas, enterobacteriaceae germs and bacteria from the acinetobacter group. There was a high incidence of infection in the tracheostomies of these patients. We found a cause and effect relationship between the contaminating bacteria isolated from the cannula and the bacteria isolated from the tracheal exudates in the case of pseudomonas aeruginosa. However, this was not found to be the case where enterobacteriaceae were concerned. Acinetobacter calcoaceticus were isolated almost exclusively in the ventilators. The study calls attention to the need for rigorous disinfection of ventilators to prevent hospital cross infections.

Acinetobacter↗
PubMedDate not supplied

Effect of a siliconized latex urinary catheter on bacterial adherence and human neutrophil activity.

The effect of a siliconized latex urinary catheter on the in vitro adherence and growth of Pseudomonas aeruginosa and Escherichia coli and on the activity of human polymorphonuclear leukocytes (PMNs) was assessed. The adherence of P. aeruginosa to latex catheters was significantly greater than that of E. coli, being 30 times higher at 6 hr of incubation. The survival of E. coli (10(8) CFU/ml) in phosphate-buffered saline (PBS) containing segments of siliconized latex catheters was lower than in the controls. This effect seems to be due to a toxic effect produced by substances eluted into the medium from the catheters, as the viability of E. coli (10(3) CFU/ml) in eluates obtained from the incubation of catheters in PBS (24 hr; 37 degrees C) was significantly lower than in the controls. These phenomena were not observed with P. aeruginosa. The incubation of human PMNs with either catheter segments or eluates did not affect the uptake of opsonized E. coli but significantly decreased the production of superoxide radicals by these phagocytes. It is concluded that the adherence of P. aeruginosa to these catheters is higher than that of E. coli and that the latex urinary catheter used herein elutes substances that are in vitro toxic for E. coli and the oxygen-dependent bactericidal mechanisms of human PMNs.

Bacterial Adhesion↗
PubMedDate not supplied

Urban clusters of sexually transmitted diseases in the city of Seville, Spain.

To test the hypothesis that there is an increased risk for sexually transmitted diseases in certain geographic areas, 613 patients diagnosed as having sexually transmitted diseases in Seville, Spain, were studied in relation to their address. Increased risks of gonorrhea (relative risk, 3.72), syphilis (relative risk, 3.32), Chlamydia trachomatis infections (relative risk, 3.41), and the three conditions together (relative risk, 3.52) were found in district 1, which represents the core of transmission and the central area of old Seville. These findings represent the first description of spatial clusters for syphilis and Chlamydia trachomatis infections.

Chlamydia Infections↗
PubMedDate not supplied

Comparative clinical efficacy of two different single-dose ciprofloxacin treatments for uncomplicated gonorrhea.

The clinical efficacies of two different single-dose, oral treatments with ciprofloxacin were evaluated in a double-blind randomized study of 50 men with gonococcal urethritis. Two groups of patients were studied. The 25 patients in group A (age, 18-32 years; mean = 26.2) received a single dose of 250 mg of ciprofloxacin; the 25 in group B (age, 16-42 years; mean = 26.3) received a single dose of 100 mg of ciprofloxacin. The minimal inhibitory concentrations of ciprofloxacin for all the isolates were less than or equal to 0.0015 microgram/ml. Neisseria gonorrhoeae was eradicated from all 50 patients; 80% of them were clinically cured, and 20% developed postgonococcal urethritis. There was no significant difference between the two treatments evaluated. We conclude that a single oral dose of 100 mg of ciprofloxacin could be an alternative treatment for uncomplicated gonorrhea in men. Neither treatment prevented the development of postgonococcal urethritis.

Adolescent↗
PubMedDate not supplied

Oral sex as a risk factor for Chlamydia-negative ureaplasma-negative nongonococcal urethritis.

We present a case-control study that evaluated the association of Chlamydia-negative, Ureaplasma-negative nongonococcal urethritis with oral and/or anal sex. The study population was composed of 120 patients diagnosed as having nongonococcal urethritis in the Diagnostic Center for sexually transmitted diseases (STD) of the Medical School of Seville. Cases were 56 men suffering from nonogonococcal urethritis from whom neither Chlamydia trachomatis nor Ureaplasma urealyticum was isolated; the remaining 64 males with cultures positive for these agents were considered as controls. Insertive genital-oral intercourse significantly increased the risk of Chlamydia-negative, Ureaplasma-negative nongonococcal urethritis (odds ratio, 11.400; 95% confidence interval, 2.357-75.057). This association was still very strong (odds ratio, 8.882) after adjustment for homosexuality. Insertive anal intercourse appears not to increase the risk of Chlamydia-negative, Ureaplasma-negative nongonococcal urethritis. We conclude that insertive oral sex can be an important risk factor in the development of nongonnococcal urethritis in which the microbial agent is still unknown.

Chlamydia trachomatis↗
PubMedDate not supplied

Clinical efficacy of new quinolones for therapy of nongonococcal urethritis.

Ninety-five patients with nongonococcal urethritis were enrolled in a double-blind study and were randomly assigned to a one-week treatment with ciprofloxacin (500 mg twice daily) or ofloxacin (200 mg twice daily). Two weeks after treatment the results obtained for the 79 evaluated patients were as follows: 59 patients (75%) were clinically cured, and of the 54 patients with initial positive cultures, 39 (72%) remained culture-negative. We did not find any statistically significant differences between the results obtained with the two treatments. These results did not vary with the cause. Patient compliance with the regimens of two doses per day was excellent, and no serious adverse effects occurred with either drug.

Adult↗
PubMedDate not supplied

Effect of phagocytosis of bacteria on the uptake of sparfloxacin by human neutrophils.

The effect of phagocytosis of Escherichia coli, Staphylococcus aureus and Haemophilus influenzae on the intracellular penetration of sparfloxacin into human polymorphonuclear neutrophils (PMN) was evaluated. The intracellular concentration of sparfloxacin was similar in PMN containing intracellular bacteria and in resting PMN [cellular to extracellular ratio (C/ E) > 8.0]. The uptake of sparfloxacin by human PMN significantly decreased during phagocytosis of opsonized H. influenzae but still reached intracellular concentrations five times greater than the extracellular ones. This effect was not observed with E. coli and S. aureus.

Anti-Infective Agents↗