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Biomedical subjects

E Johannisson

Publications and source records attributed to E Johannisson.

At least 73 records · Page 4Linked to original sources
PubMed1983

The effect of a prostaglandin synthetase inhibitor on the hormonal profile and the endometrium in women.

A prostaglandin synthetase inhibitor, naproxen, was given continuously throughout the menstrual cycle at a dose of 250 mg b.i.d. to 10 healthy fertile women. Blood samples were drawn three times a week during an earlier control cycle and during the treatment cycle and analyzed for LH, FSH, prolactin, estradiol and progesterone. Endometrial biopsies were taken in the secretory phase. Naproxen treatment did not suppress ovulation in any of the cycles and did not affect the corpus luteum function. In only one of the endometrial samples taken was the density of the lysosomes increased in the treatment cycle compared with the control cycle.

Endometrium↗
PubMed1982

A preliminary pharmacological trial of the monthly injectable contraceptive cycloprovera.

A comparative pharmacological pilot study of the monthly injectable contraceptive CycloProvera was carried out in 11 women in four centres. There were no significant differences in the results between the centres except that the injection-bleeding interval appeared to be shorter in Swedish women than in those in Havana and Mexico. Medroxyprogesterone acetate was detectable in blood for 28 to 62 days after injection of CycloProvera and although follicular activity returned in less than 28 days after injection in many of the women, corpus luteum function was suppressed for at least seven weeks in all women. Most of the women retained a regular menstrual pattern; six of 33 cycles were amenorrhoeic. There was no significant change in any of the biochemical and haematological analyses.

Adult↗
PubMed1982

Pharmacokinetic and pharmacodynamic investigations with vaginal devices releasing levonorgestrel at a constant, near zero order rate.

The pharmacokinetic and pharmacodynamic effects of vaginal devices releasing levonorgestrel (L-NOG) at a constant rate (in vitro release: approximately 20 micrograms/day) were studied in a group of 20 normally menstruating women during a period of 90 days of continuous use. Peripheral blood samples were withdrawn two or three times (in the great majority of subjects, on Mondays, Wednesdays and Fridays) during a pretreatment (control) cycle, during 90 days of exposure and during the first week following the removal of the device, and the levels of L-NOG, estradiol and progesterone were analyzed by radioimmunoassay techniques. In 8 of these subjects, endometrial biopsies were also taken during days 23 to 25 of the control cycle, and then 6 and 10 weeks following the insertion of the devices. In addition, the initial absorption rate and removal half-life of L-NOG were assessed in 7 subjects using the devices for a period of 8 days only. Following insertion of the devices, the levels of L-NOG rose very rapidly, and reached the final "plateau" in some 30 minutes' time. This was followed by a limited period of "burst" with doubling the levels for a few hours, after which the levels remained stable and diminished very slowly in a linear fashion with an average decline of 23-26% during 90 days, corresponding to a daily decrease of 0.2 to 0.3 per cent. The removal half-life (first compartment) after 90 days of exposure in 19 subjects was 16.1 (13.7-18.6) hours. Sixty-nine treatment segments of 30 days were studied with frequent hormone assays; of these, 20 (or 29%) were anovulatory, 13 (19%) exhibited inadequate luteal function, and 32 (52%) had normal ovulatory-like cycles. All endometrial biopsies obtained during the pretreatment cycle were normal secretory; of the 16 biopsies obtained during treatment, 4 were suppressed, 2 proliferative, 6 secretory, and 4 could not be dated because of bleeding. An assessment of the bleeding profiles during exposure to L-NOG revealed almost a doubling of the number of days with bleeding and spotting (35% compared to 18% during the pretreatment cycle). However, significantly more frequent bleeding was found in the 20 anovulatory segments (37.3%) than in the 36 normal ovulatory-like ones (27.7%). It is concluded that differences in the frequency of bleeding and spotting with low-dose progestogens may reflect differences in the frequency of ovulation inhibition just as much as differences in the hormonal profiles of the compounds administered.

Adult↗
PubMed1982

Morphometric analysis of the human endometrium in relation to peripheral hormone levels.

Twelve indices were quantitated at three sites in 68 endometrial biopsies from 14 women in five cycles each in which daily plasma levels of luteinizing hormone (LH), estradiol (E2), and progesterone (P) were measured. It was found that a single biopsy specimen is representative of the entire endometrium. Using classic dating, 59% of the biopsies were correct. The error rate increased with the distance from the LH surge. The morphometric indices revealed significant differences am ong all 48-hour secretory phase periods except days LH + 7 to LH + 10. The glandular diameter was positively correlated with proliferative phase E2 levels and with secretory phase P levels; the latter were negatively correlated with stromal mitoses. Basal vacuoles were negatively correlated with E2 and positively with P levels.

Adult↗
PubMed1982

Microfluorometric assessment of sperm maturation in testicular biopsies from men with histologically normal or reduced spermatogenesis.

Spermatogenesis has been studied in testicular biopsies by means of microfluorometric assessment of the DNA-DNP complex using ethidium bromide in 10 men with histologically normal spermatogenesis and in 10 men with histologically reduced spermatogenesis. The expected haploid DNA value was found in the round spermatids, whereas only 70% of the haploid fluorescence value was found in the elongated spermatids and around 60% in the testicular spermatozoa. No difference was found in the mean fluorescence values between the pathological group and the controls. it is suggested that structural changes or an increase in basic nuclear proteins gradually exclude the ethidium bromide from the binding to the DNA molecule and that this phenomenon occurs concomitantly with a decrease in double-stranded RNA. The difference in fluorescence intensity before and after RNAase treatment was regarded as an approximate estimate of the RNA content. There was a significant decrease of these values during spermatogenesis.

Adult↗
PubMed1981

Regeneration of the human endometrium and presence of inflammatory cells following diagnostic curettage.

The regeneration of the human endometrium, with particular emphasis on the presence of inflammatory cells, was studied in 106 women undergoing hysterectomy following curettage. The interval between the last curettage and the hysterectomy varied from 1 day to 12 months. A slight but significant increase in the number of neutrophil cells was observed during the first 7 days after the curettage in proliferating endometrium. This increase was considered to reflect the regeneration phenomenon rather than a true infection. No statistically significant increase in the number of neutrophils and mononuclear cells was observed after the first week following the curettage. The study does not provide any evidence that the introduction of a curette or a suction instrument in the uterine cavity increases the risk of endometritis.

Adult↗
PubMed1980

Microfluorometric grading of mammary tumors. A pilot study.

Material obtained from 24 women with benign and malignant breast lesions was subjected to comparative morphologic and cytophotometric DNA analysis. All the cases of benign tumors, as well as benign tissue samples obtained from tumor-containing breasts, exhibited DNA values corresponding to the diploid control value. The tumors classified as carcinoma in situ or highly differentiated invasive cancers (grade I) showed a distribution of the DNA values significantly different from the diploid control values. The tumors classified s grade II and III showed a shift to the "triploid" area or were totally aneuploid. The results of the present pilot study suggest that quantitative cytophotometric DNA analysis can be used for grading the malignancy of breast tumors in combination with conventional histologic examination. Additional studies of larger groups of women may yield further information on the microfluorometric grading of malignancy. Such studies may also contribute to the further development and use of automated methods of cytologic diagnosis.

Adult↗
PubMed1977

Mithramycin fluorescence for quantitative determination of deoxyribonucleic acid in single cells.

The use of the antibiotic drug mithramycin for cytoflurometric assessment of deoxyribonucleic acid in single cells has been studied in smears of a standard cell population of rat thymocytes. The optimal staining conditions have been determined including the influence of fixation (freeze-drying, formalin and ethanol). The drug equilibration time has been estimated in relation to the concentration of the mithramycin and to Mg++ was found to enhance the fluorescence intensity produced by the mithramycin-deoxyribonucleic acid interaction. The stability and the reproducibility of the fluorescence reaction are discussed.

Animals↗