The cytochemical properties of the human endometrial cell nuclei have been studied during the menstrual cycle in eight women by means of two different microfluorometric methods, both considered to be specific for DNA: the BAO-Feulgen method and a method based upon an interaction between DNA and mithramycin. An in vitro system was developed where dissociated endometrial cells were studied at 4 hours and 18 hours of incubation at 37 degrees C. The cytochemical properties of the dissociated endometrial cells did not change significantly in the in vitro system used. An increase in the BAO-fluorescence intensity per cell nucleus was observed in all the endometrial samples when compared with the diploid standard DNA value of a non-growing cell population. A similar increase in the mithramycin fluorescence intensity per cell nucleus appeared only during the secretory phase. It is suggested that the nucleoproteins play an essential role in changing the cytochemical properties of the human endometrial cell nuclei during the menstrual cycle.

Analysis of Variance↗

PubMedDate not supplied

The effect of intrauterine progesterone on the DNA-content in isolated human endometrial cells.

The effect of an intrauterine progesterone-releasing device on the DNA-content in human endometrial cells has been studied by means of a microfluorometric method. Following six of seven months' use of the progesterone-releasing device a significant decrease in the mean DNA-content per cell nucleus was found in the epithelial cells indicating a suppression of the proliferative activity of the endometrium. The mean DNA-values of the endometrial cells remained at a low level throughout the cycles in spite of circulating levels of steroids indicating a normal ovarian function. It is suggested that the progesterone released in utero interferes with the DNA-synthesis of the epithelial cells, which may result in a disturbed metabolic function of the endometrium at the time of implantation.

Adult↗

PubMedDate not supplied

The effect of intrauterine copper on the DNA-content in isolated human endometrial cells.

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Adult↗