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Biomedical subjects

E Krüger

Publications and source records attributed to E Krüger.

At least 37 records · Page 2Linked to original sources
PubMed1994

Immunohistochemical detection of p53 protein in human prostatic cancer.

Tissue sections from 73 radical-prostatectomy specimens were studied immunohistochemically for the presence of p53 protein. In seven specimens numerous tumor cells showed a strong nuclear immunostaining. An additional 27 revealed a more discrete and focal accumulation of p53 protein. Comparison of the pathologic characteristics of the p53-negative and -positive groups showed that the presence of p53 protein closely correlated with more advanced tumor stages (p < 0.00001), with higher primary (p = 0.0004), combined (p < 0.0001) and worst (p < 0.0001) Gleason grades, and with larger total (p = 0.0001) and high-grade (p < 0.0001) tumor volumes. No staining was found in areas of benign hyperplasia or in well-differentiated tumor zones. Our results suggest that the accumulation of p53 protein to immunohistochemically detectable concentrations is not a feature of low-grade cancer. This finding implies that abnormal p53 accumulation might be involved in the process of prostatic cancer progression.

Aged↗
PubMed1994

Stress induction of clpC in Bacillus subtilis and its involvement in stress tolerance.

A member of the clpC subfamily of stress response-related Clp ATPases was cloned from Bacillus subtilis. The B. subtilis clpC gene was induced in response to various stresses, including heat shock. Its product was identified as a general stress protein (Gsp12) described previously. A dramatic increase in the amount of clpC mRNA immediately after exposure to multiple stresses suggested regulation on a transcriptional level. Induction by heat shock was independent of the alternative sigma factor SigB, indicating a new mechanism of heat shock induction in B. subtilis. A clpC insertional mutant had an impaired tolerance for heat shock and salt stress. Furthermore, the mutation triggered the formation of elongated cells, a phenomenon particularly pronounced during stress.

Adaptation, Biological↗
PubMed1994

[Mandibular reconstruction with a free avascular iliac crest transplant].

119 avascular osseous transplants from the iliac crest were used for mandibular reconstruction. In case of irradiation we prepared the region of transplantation by using myocutaneous flaps. We lost 2.5% of our transplants. Analysing the orthopantomogram after a special method regarding reproducibility, we found a loss of height of the transplant of about 30% in the middle term. During rigid fixation of the transplant in the first 3 month, there was the biggest loss of height. Transplants ending free in the region of the TMJ showed less consistency than transplants interposed between original mandibular bone.

Adolescent↗
PubMed1993

Numerical chromosomal aberrations in prostate cancer: correlation with morphology and cell kinetics.

Eleven routinely processed radical prostatectomy specimens were studied for the presence of numerical chromosomal aberrations by means of in situ hybridization with nucleic acid probes specific for chromosomes 7, 10, 17, X, and Y. Cytogenetic information was correlated with morphology, tumour stage and volume as well as with cell kinetics, the latter being assessed by immunohistochemistry with antibodies raised against the proliferative cell nuclear antigen (PCNA) and against a formalin-resistant epitope of the Ki-67 antigen, MIB 1. In 5 of 11 cases, numerical aberrations of at least one chromosome were found. The cases with normal chromosome numbers were those with the smallest volumes of Gleason grade 4 and/or 5 tumour (mean 0.5 cm3) and represented tumours restricted to the prostate. Tumours with aberrations in the number of detected chromosomes showed advanced stages and large volumes of high-grade tumour (mean 12.5 cm3). All 4 tumours with positive surgical margins were recruited from a group with marked local heterogeneity in chromosome numbers. Immunostaining with MIB 1 and PCNA was most intense in areas of high-grade tumour and was positively correlated with the emergence of chromosomal aberrations. The data suggest that the appearance of numerical chromosomal aberrations in prostate cancer coincides with aggressive tumour behaviour and could be used as an additional prognostic marker.

Aged↗
PubMed1993

[Significance of numerical chromosome aberrations in prostate cancer].

Thirty-one routinely processed radical prostatectomy specimens were examined for the presence of numerical chromosomal aberrations by in situ hybridization with centromeric nucleic acid probes specific for chromosomes 7, 10, 17, X, and Y. In eight of the prostatectomy specimens, chromosome numbers consistent with a normal male karyotype were found. Three cases, in addition to diploid chromosome numbers, showed a focal doubling of hybridization signals, consistent with tetraploidy. The other 20 cases displayed more or less marked numerical chromosomal aberrations. In this group, the appearance of numerical chromosomal aberrations often showed considerable local heterogeneity and was significantly correlated with tumor stage, Gleason grades, and tumor volume. We conclude that in prostatic cancer the presence of numerical chromosomal aberrations is associated with advanced disease. Especially in low differentiated tumors local heterogeneity in chromosome numbers can be very marked.

Chromosome Aberrations↗
PubMed1993

[Gigantic pyohydronephrosis and contralateral ureteral bud. Problems of differential diagnosis].

Both giant hydronephrosis and blind-ending ureteral bud are rare conditions. We report on a patient with an impacted ureteral stone leading to giant pyohydronephrosis (6.51) on the right side, which in turn caused displacement of the ureter containing the stone to the left. An asymptomatic blind-ending ureteral bud initially complicated the diagnosis. After nephrectomy, a unsuspected transitional cell carcinoma of the right renal pelvis and the ureter was found.

Carcinoma, Transitional Cell↗
PubMed1991

T7 infection-dependent selective expression of cloned genes in P1-lysogenic Escherichia coli.

Expression systems based on the selective transcription of genes cloned behind a T7 promoter, by T7 RNA polymerase, display a non-negligible basal expression when the T7 RNA polymerase gene is present within the host organism before induction of the system. This is a problem, especially for cloning and controlled expression of genes toxic to the host organism. We have circumvented this problem by taking advantage of abortive T7 infection of E. coli (P1), in the course of which T7 RNA polymerase is synthesized but bacterial growth is not quantitatively impaired. We have tested this system with three reporter genes, the 6-phospho-beta-galactosidase gene of Staphylococcus aureus, the luciferase operon of Vibrio harveyi, and the rabbit beta-globin gene; we have found very low basal levels, while, upon T7 infection, transcription is at least as efficient as in other in vivo T7 RNA polymerase systems in use.

Animals↗
PubMed1991

Dihydrolipoic acid activates oligomycin-sensitive thiol groups and increases ATP synthesis in mitochondria.

Investigations with dihydrolipoic acid in rat heart mitochondria and mitoplasts reveal an activation of ATP-synthase up to 45%, whereas ATPase activities decrease by 36%. In parallel with an increase in ATP synthesis oligomycin-sensitive mitochondrial -SH groups are activated at 2-4 nmol dihydrolipoic acid/mg protein. ATPase activation by the uncouplers carbonylcyanide-p-trifluoromethoxyphenylhydrazone and oleate is diminished by dihydrolipoic acid, and ATP synthesis depressed by oleate is partially restored. No such efficiency of dihydrolipoic acid is seen with palmitate-induced ATPase activation or decrease of ATP synthesis. This indicates different interference of oleate and palmitate with mitochondria. In addition to its known coenzymatic properties dihydrolipoic acid may act as a substitute for coenzyme A, thereby diminishing the uncoupling efficiency of oleate. Furthermore, dihydrolipoic acid is a very potent antioxidant, shifting the -SH-S-S- equilibrium in mitochondria to the reduced state and improving the energetic state of cells.

Adenosine Triphosphatases↗
PubMed1991

[Acute poisoning caused by the herbicide dichlorprop (preparation SYS 67 PROP)].

We report on the acute poisoning of a 5-year-old boy by the herbicide "dichlorprop". This preparation is described as an effective means of plant protection. The effects of the intoxication were established by changes observed in almost all of the child's organs. The predominant effect was haemolytic anemia. All the pathological parameters regressed as a result of symptomatic therapy. General conclusions are drawn about intoxication, especially by means of plant protection agents.

2,4-Dichlorophenoxyacetic Acid↗
PubMed1990

[Conservative therapy of mandibular fractures in children with operatively secured splints].

In the treatment of mandibular fractures in children offering no adequate possibility for attaching splints to the teeth, we use prosthetic splints fixed with four circumferential wires. This monomaxillary fixation is sufficient to stabilize fractures of the mandibular anterior segment. Fractures situated distal to the canines require additional intermaxillary fixation, i.e. zygomaticomaxillary suspension of the mandible. When functional therapy is needed at the same time, we use an activator instead of the prosthetic splint. 59 patients treated in this way were reevaluated within a period of 10 years. The results of this reevaluation are reported.

Child↗
PubMed1990

[Neuromuscular diseases and respiratory insufficiency in newborn infants].

Myopathological examinations which served to gain information on the potential impact of neuromuscular disease on the syndrome of perinatal respiratory failure included 47 consecutively deceased premature infants and neonates. A definite muscular disease could not be safety diagnosed in any case. However, only a very small number of investigations and data on the initial (possibly prenatal) manifestations have been available up to now. We found a total of 11 cases with increased intrafibrillary fat deposits coupled with mitochondrial aggregations in three cases. In seven cases, we found a retarded maturation ranging from three to nine weeks. Focally manifest myopathic changes were recorded in three cases. In one case, a large-group atrophy was found which might indicate the existence of a defect in the region of the second motor neuron. Biochemical backup investigations are necessary to be able to assess the relatively high incidence of increased fat deposits. We consider myopathological examinations of premature infants who died of respiratory failure to be desirable because far more comprehensive studies are necessary to be able to assess the importance of perinatally occurring muscular changes for the diagnosis of neuromuscular diseases.

Humans↗
PubMed1989

Stabilization of maxillary segments and dental arch after puberty, in cleft lip and palate cases.

To overcome the high tendency to relapse after orthodontic treatment in patients with cleft lip and palate, efficient stabilisation of the dental arch is essential. In patients with an alveolar defect, extended bridges are most suitable, because there is no need for further retention appliances, the functional and cosmetic results are acceptable and extensive prosthetic reconstruction can be avoided, regardless of the patients age.

Adolescent↗
PubMed1989

[Psychiatric long-term hospitalization--and how does life go on?].

Problems of longterm psychiatric hospitalisation are represented at the example of the topical supply situation in the mental hospital Bezirksnervenklinik Haldensleben. By means of a shown catalogue of symptoms from longterm psychiatric hospitalised patients tasks of care and inferences are discussed under the aspect, that the living and therapy conditions respectively their structures of the psychical sick persons authenticately show reactions on the course of illness and attendance. A lot of graduated care-offers in form of a therapeutical network first of all open a social reintegration in regard of individual and reality. At the same time hence it follows the chance for modifications in the psychiatric hospital.

Deinstitutionalization↗
PubMed1989

[Epidemiology of generalized periodontal atrophy].

1378 individuals of 15-44 years of age were examined for atrophic processes of the periodontia. 11% of the individuals showed typical inflammation - free gingival recession. Great importance must be attributed the McCall's festooms and the wedge shaped defects in cervical enamel, dentin or cement.

Adolescent↗