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Biomedical subjects

E Kuthy

Publications and source records attributed to E Kuthy.

9 recordsLinked to original sources
PubMed1979

Renal cortical tubules in experimental malakoplakia. Phagocytic alteration of tubular epithelium.

Intrarenal injection of a crude E. coli extract (endotoxin-antigen-complex) induced malakoplakia in rats. Beside the granulation tissue the proximal tubular epithelium showed a strong phagolysosomal response--especially in two-three weeks--, thus becoming very similar to the Hansemann cells of the malakoplakia granulation tissue. This malakoplakia alteration of the epithelium if very severe sometimes led to necrosis or it was segregated inside the epithelial cells. Later on an atrophy of the tubules developed similar to the atrophy of different etiology, but the remaining cells often contained a striking number of residual bodies. It is suggested that the tubular granulated cells of megalocytic interstitial nephritis regarded as identical with renal cortical malakoplakia also have a tubular epithelial origin.

Acid Phosphatase↗
PubMed1979

[Transformation of the epithelial cells of the kidney tubules in experimental malacoplakia].

As an effect of administration of the extract of E. coli in the kidney of rats phagolysosomal reaction has been observed, not only in the granulomatous tissue characteristic of malacoplakia, but in the epithelial cells of the proximal tubules. The latter became similar to the histiocytes of malacoplakis i.e. Hansemann's cells. The transformation of the tubular epithelium in severe cases of malacoplakia was followed by necrosis. In a later phase tubular atrophy came into being, which did not differ from the atrophy caused by other agents, although epithelial cells contained numerous residual body. Authors believe, that granular tubular cells of megalocytic interstitial nephritis--regarded as malacoplakia of the renal cortex--originate from tubular epithelium.

Animals↗
PubMed1978

[X-ray microanalysis of Michaelis-Gutmann bodies with the use of EDAX].

X-ray microanalysis of Michaelis--Gutmann bodies in human malakoplakia (kidney and testes) and in that of rats induced experimentally by administration of endotoxin of Escherichia coli, was carried out. The presence of calcium could be revealed in every Michaelis--Gutmann body according to the lines of Kalfa and K3 as well. The amount of it was in correlation with the stage of the calcification. In the Michaelis--Gutmann bodies found in the rat kidney, fixed without OsO4 presence of P could also be demonstrated. The correlation between the weight per cent of Ca and P seems to evidence the presence of CaHPO4. Results of the X-ray microanalysis of Michaelis--Gutmann bodies found in human and in experimentally induced malakoplakia appeared to be similar.

Antitoxins↗
PubMed1978

X-ray microprobe analysis of Michaelis-Gutmann bodies in human and experimental malakoplakia.

Using x-ray microprobe analysis, we studied Michaelis-Gutmann bodies (M-G bodies) in malakoplakia tissue samples from human kidney and testis as well as from lesions experimentally induced in the rat. In all the M-G bodies tested we could unambiguously detect calcium at both its Kalpha and Kbeta lines. The actual amount of calcium depended on the developmental state, ie, on the degree of calcification of the M-G body, In M-G bodies of rat kidney malakoplakia tissue fixed without osmium tetroxide, the phosphorus could also be detected unambigously. From the ratios of calcium and phosphorus percentages (w/w) we concluded that CaHPO4 was present. No difference was found between M-G bodies in malakoplakia tissues of human origin and those in experimentally induced malakoplakia.

Animals↗
PubMed1976

Peculiar artefacts after fixation with glutaraldehyde and osmium tetroxide.

Peculiar electron dense granules were observed, in otherwise well preserved structures of the heart muscle and kidney of the rat after a 5-10 min perfusion, a 2 h fixation in the modified Karnovsky solution, followed by a postfixation in Millonig buffer containing 1% OsO4. The granules appeared most frequently on membranes, the surface of the red blood cells and in pinocytotic vacuoles, in stained and unstained sections alike. No artefacts were produced when the same buffer was used for both pre- and postfixation, or when the postfixative contained Veronal buffer.

Aldehydes↗
PubMed1975

Experimentally induced malakoplakia.

Malakoplakia was induced experimentally by introducing large amounts of crude endotoxin-antigen comples of 075 Escherichia coli (E. coli 12797 CDC 0 group 75) into the kidneys and testes of rats. First leukocytes, then granulation tissue composed mainly of characteristic macrophages, the so-called Hansemann cells, appeared around the endotoxin-antigen mass. On the eighth day following the injection, deposition of calcium phosphate into the cytosegresomes of macrophages began and acused the formation of the Michaelis-Gutmann bodies necessary for the diagnosis of malakoplakia. The induction of the same process in humans by E coli endotoxin seems to be possible.

Animals↗
PubMed1975

[Characteristics of artefacts following fixation with glutaraldehyde and osmium tetroxide].

In the myocard and kidney of rats perfused with Karnovsyk's solution for 5--10 minutes then fixed for two more hours and postfixed in 1 per cent solution of osmium tetroxid in Millonig buffer, characteristic electrondense granules were observed. The structure of the organ appeared to be normal. Although the granules appeared to be diffuse, they presence was most marked on the membranes, on the surface of erythrocytes and in the pinocytic vacuoles both on counterstained slides and on those of without counterstaining. When postfixation was carried out with osmium-tetroxide solved in Veronal buffer artefacts did not occur. To avoid artefacts fixation in glutaraldehyd should not be followed by a postfixative containing phosphate buffer.

Aldehydes↗
PubMed1975

[Experimental malakoplakia in the kidney].

Malakoplakia in kidney of rats was caused experimentally by injection of large quantities of not purified endotoxin-antigen complex of Escherichia coli 0 75 (O. coli 127 97 CDC 0 group 75; American Type Culture Collection, Eight Edition 1968, page 24). The extract in the beginning became surrounded by leukocytes, later by macrophages. The latter then were transformed into characteristic Hansemann cells. From the 8th day on started the depletion of calcium phosphate in the cytosegresomes of macrophages, thus forming the Michaelis-Guttmann bodies, necessary for the diagnosis of malakoplakia. It is believed that complex of endoxin- and antigen of Escherichia coli may take a part - at least in certain cases - in formation of malakoplakia in human.

Animals↗