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Biomedical subjects

E L Dovedova

Publications and source records attributed to E L Dovedova.

At least 19 recordsLinked to original sources
PubMed2006

[The peculiarities of long-term amphetamine action on some brain enzyme systems].

The enzyme activity of serotonin and dopamine synthesis--tryptophan hydroxylase and tyrosine hydroxylase respectively--as well as content of dihydroxyphenylalanine (DOPA) in the brain structures of Wistar and August rat strains were studied in normal conditions and during long-term amphetamine administration (21 day) in dosage of 1.0 mg/kg. Between-strain differences were observed both in normal conditions and during amphetamine using. Inhibition of tyrosine hydroxylase activity, decrease of DOPA and activation of tryptophan hydroxylase activity in the brain structures were found in August rats under amphetamine action. On the contrary, inhibition of tryptophan hydroxylase activity, activation of tyrosine hydroxylase activity and increase of DOPA were revealed in Wistar rats. A relationship between neuronmediator systems metabolism in the brain and peculiarities of behavior and emotional reactivity of the animals during pathological influence was demonstrated.

Amphetamine↗
PubMed2005

Effect of delta-sleep-inducing peptide on activity of enzymes of biogenic amine metabolism in the brain of Wistar and August rats.

Activity of enzymes catalyzing synthesis and degradation of serotonin and dopamine in brain structures of Wistar and August rats was measured biochemically under normal conditions and after short-term exposure to delta-sleep-inducing peptide. The effects of the test peptide manifested in activation of the serotoninergic system and inhibition of the dopaminergic system, particularly in the caudate nucleus. These changes were most pronounced in the brain of Wistar rats.

Animals↗
PubMed2004

[Morphochemical characteristic of rat brain structures after exposure to delta-sleep inducing peptide following long-term amphetamine administration].

The aim of this work, that was carried out using Wistar rats, was to characterize the response of neurons of different morphofunctional types to amphetamine administration and to study the possibility of correction of these changes by delta-sleep inducing peptide (DSIP). Single intraperitoneal injection of 60 microg/kg of DSIP following a long term amphetamine administration (2.5 mg/kg for 3 weeks) was shown to result in normalization of brain metabolism, that was disturbed by the drug. The correcting DSIP effect was found in rat brain structures judging by the parameters of the state of proteins in neurons of sensomotor cortex and caudate nucleus and by the activity of enzymes of neurotransmitter metabolism, such as type A and B monoamine oxidases and acetylcholine esterase, that was determined in subfractions of the same brain structures. DSIP modulating effect in phenamine stereotypy supports its role as an adaptogen of intercenter relations in CNS pathology.

Acetylcholinesterase↗
PubMed2003

[Mechanism of nootropic digam action in hypofunction of brain dopaminergic system].

Using microchemical methods for detection of dopamine (DA), noradrenaline (NA), serotonin (S) and its metabolite--5'-hydroxyindolilacetic acid (5'-HIAA) as well as the activity of neuromediator-utilising enzymes--MAO A and B and enzymes of acetylcholine metabolism--cholinacetyltranspherase (ChAT) and acetylcholinesterase (AChE), we revealed that synthetic GABA-derivative compound diagram (250 mg/kg during 10 days) normalized functioning of dopaminergic and acetylcholinergic systems in sensormotor cortex and caudate nucleus of Wistar rats with haloperidol-induced (0.5 mg/kg during 30 days) bradykinesia. Measured by quantitative interpherometric method, a specific response of functionally different sensomotor cortex (layers III and V) neurons and caudate nucleus by such characteristics as cytoplasm and nuclei sizes, protein content and concentration was found. Control for rat's behavior in open field revealed that diagram restored emotional activity disturbed by haloperidol injections and improved the indices of the animals searching activity.

Animals↗
PubMed2003

[Amphetamine action on morphochemical brain organization].

The activity of enzymatic systems--monoamine oxidase, types A and B, and acetylcholinesterase involved in neuromediator utilisation and their levels correlation in the cortex and caudate nucleus brain tissue subfractions in control and during long (3 weeks) amphetamine (psychostimulator) injections in dosage 2.5 mg/kg were studied. Wistar and August rats different by behavioral characteristics were used. Differences of enzyme activity on subcellular level both in controls and in rats with dopaminergic dysfunction, caused by amphetamine, were found. Comparing to Wistar rats, in August ones characterized by lower motor activity and elevated stress sensitivity, amphetamine caused a significant MAO A and acetylcholinesterase activity inhibition, the changes being more pronounced in the caudate nucleus. It is suggested that the differences of enzymatic activity, reflecting the state of brain neuromediator metabolism and emerging in pathological conditions, may underlie a mechanism of CNS psasticity.

Acetylcholinesterase↗
PubMed2000

Metabolism of neurotransmitters in cortical and subcortical brain structures in rats with different behavioral characteristics.

Experiments were performed on Wistar rats with high and low locomotor activities. In rats with high locomotor activity, activities of acetylcholine transferase, acetylcholine esterase, and monoamine oxidase A increased in the subcellular fractions of the sensorimotor cortex and arcuate nucleus, while monoamine oxidase B activity decreased compared to those in rats with low locomotor activity. The peculiarities of neurotransmitter systems in brain structures of rats with different behavioral patterns were related to genetic and functional organization of the central nervous system.

Animals↗
PubMed1999

Regulation by delta-sleep-inducing peptide of the neurochemical changes in the brain associated with dopaminergic system hyperactivity.

The influence of a single injection of "delta-sleep-inducing peptide" (DSIP; 30 microg/kg body weight) on neurochemical parameters of rats' brain was studied under the conditions of chronic administration of dopamine analogs inducing DA-system hyperactivity - 50 mg/kg body weight of L-DOPA for 30 days or 2,5 mg/kg body weight of amphetamine for 21 days. The parameters of serotonergic system (MAO A activity, 5-HT, and 5-HIAA contents) and of dopaminergic system (MAO B activity, DA, NA, and HVA contents) were investigated in the cortex and caudate nucleus of control, DA or amphetamine, and DSIP receiving rats. Changes caused by the two DA-system activating drugs had both similarities and differences, and the corrective action of DSIP also had certain peculiarities depending on the pharmacological preparation used for the induction of DA-system hyperactivity and on the investigated brain structure. It is supposed that DSIP action might be based on the activation of serotonergic system that ensures the adaptive behavior of the animals.

Amphetamines↗
PubMed1999

[The activity of the neuromediator systems in the cortex and caudate nucleus in rats with different degrees of initial alcohol preference].

The study was made of functional state of catecholamine, dopaminergic, serotonergic and cholinergic cerebral systems in terms of the activity of some enzymes of neuromediator metabolism, the content of biogenic amines and their correlation in brain and cerebral caudate nucleus. 3 groups of rats with different initial craving for alcohol were examined: rejecting alcohol (RA); consuming it but without preference (WAP); and rats preferring alcohol under the conditions of free choice between water and alcohol (PA). Peculiarities of the functional state of the systems studied depended on the degree of the preference to alcohol. The main changes in PA rats, as compared with RA group, manifested as a decrease of corresponding indices. That confirmed a different degree of the inhibition of their activity as well as alterations in the interaction between neuromediator systems. These changes were more pronounced in the cortex than in caudate nucleus. Such alterations may be related to structural-functional flexibility of CNS and might be the basic mechanism of the genetic predisposition to alcohol consumption.

Alcohol Drinking↗
PubMed1997

[The correction of amphetamine action by the delta sleep-inducing peptide].

Effects of "delta-sleep inducing peptide" administration (60 micrograms/kg) to rats on the background of amphetamine action (2.5 mg/kg during 3 weeks) were studied. Activities of the enzymes of neurotransmitter turnover as well as the contents of biogenic amines in structures of motor cortex and caudate nucleus were investigated. Normalizing action of the peptide on the studied indices was found. It reflected the correction of the increased activity of dopaminergic and serotoninergic systems elevated as a result of amphetamine action. The suggestion was made that reconstruction of the interactions of neurotransmitter systems in brain providing adaptive behavior in animals was the main mechanism of delta-sleep inducing peptide's action.

Amphetamine↗
PubMed1996

[The morphochemical characteristics of the reaction of the motor structures of the brain to hyper- and hypofunction of the dopaminergic system].

The investigation of protein (aminopeptidase activity, structured proteins) and of mediator metabolism (monoaminoxidase A and B activities, acetylcholine esterase, Ca(2+)-uptake) was carried out in rat brain motor structures. The study was performed during chronic L-DOPA and haloperidol administration that is under the conditions of hyper- and hypofunction of dopaminergic system. Some peculiarities and differences of sensomotor cortex and nucleus caudatus' reactions to the dysfunction of DA system were revealed at the cellular and subcellular levels. The results show the existence of high morpho-biochemical plasticity of brain structures mentioned above.

Animals↗
PubMed1994

[Multilevel neurochemical organization of the brain].

Many years' studies in functional neurochemistry have provided a concept of the functionally induced biochemical heterogeneity of neurons and their subcellular components, which is formed during ontogenesis as the functions of the central nervous system are developed. It has been established that it manifests itself at the systemic, cellular, and subcellular levels: (1) between brain formations which constitute the single functional system; (2) between the neurons of various morphofunctional types (associative, efferent, afferent ones) between the neurons and the glia; (3) between individual components (the nucleus and the cytoplasm) of a neuron and its subcellular (synaptosomes, mitochondria, synaptosomal membranes) components. It is concluded that the functionally determined biochemical heterogeneity and morphofunctional features found in animals of various strains may be regarded as one of the ways of compensatory, rehabilitative, and adaptative processes in the central nervous system.

Animals↗
PubMed1993

[The mechanism of the pathogenesis of drug-induced parkinsonism (experimental biochemical and bioelectrical research on the action of haloperidol)].

Haloperidol was administered to rabbits in a dose 500 micrograms/kg during 60 min or 30 days. Its effects were studied in enzymes controlling neurotransmitters utilization--monoamine oxidase (MAO) A and B, acetylcholine esterase (ACE) in synaptosomes subfractions and mitochondria of the cells--and on the content of biogenic amines in the sensomotor cortex and caudate nucleus tissues. It was found that this neuroleptic induces reciprocal changes in MAO specific activity (a decrease in MAO B activity and an increase in MAO A), a fall in the levels of dopamine and noradrenaline in unchanged levels of 5'-HT and 5'-HYAA.

Animals↗
PubMed1989

[Functional and biochemical reorganization in hyperfunction of the dopaminergic system].

In dogs, cats, rabbits, the effects of unique and chronic administration of L-DOPA (20-50 mg/kg daily) on central dopamine processes, behaviour, interrelations of sensory and motor regimens of the structures and on the transmitter metabolism, were studied. The interrelations of sensory and motor regimens became competitive, reciprocal changes occurred in dopaminergic and other transmitter (serotoninergic and acetylcholinergic) systems. The findings seem to be related to development of psychomotor excitation.

Acetylcholinesterase↗
PubMed1989

[Mechanism of the action of the delta sleep-inducing peptide against a background of L-DOPA administration].

A reciprocal nature of the shifts in activity of type A and B monoamine oxidases has been observed under the effect of DSIP against the background of L-DOPA administration (50 micrograms/kg) in the subfractions from the rabbit sensorimotor cortex. The results suggest that the activation of type A monoamine oxidase and serotoninergic system is the basis of the adaptive behavior of animals.

Acetylcholinesterase↗