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Biomedical subjects

E M Shelukhina

Publications and source records attributed to E M Shelukhina.

At least 37 records · Page 2Linked to original sources
PubMed1976

An antigenically atypical strain of variola virus.

The Kuwait-5-67 strain of variola virus, although indistinguishable from variola virus in its other properties, behaved atypically in immuno-diffusion tests with antivaccinia serum. The precipitation line pattern produced was similar to that of cowpox virus but lacked one of the main precipitation bands given by all other strains of variola, alastrim and vaccinia viruses tested. Trypsin treatment of cowpox virus preparations revealed the missing precipitation line but did not do so with the Kuwait-5-67 strain. The antivaccinia serum absorbed by Kuwait-5-67 strain lost its ability to interact with this strain, but still gave precipitation lines with variola, vaccinia and cowpox viruses.

Antigens, Viral↗
PubMed1976

[Use of the passive hemaglutination reactions for the determination of anti-smallpox antibodies in primary vaccination and revaccination against smallpox].

The authors present the results of studying the PHAT sensitivity in comparison with the neutralization test and the hemagglutination inhibition test in examination of 254 sera of revaccinated and 95 sera of primarily vaccinated children. It appeared that PHAT was characterized by a sufficiently high sensitivity, and reproducibility, in case the same batch of erythrocytic diagnostic agent was used; at the same time the test was simple, accessible, economic, and gave rapid results. This test can be used for assessment of the immunological efficacy of smallpox revaccination.

Antibodies, Viral↗
PubMed1976

"White-wild" (variola-like) poxvirus strains from rodents in Equatorial Africa.

Strains of variola-like virus were isolated from rodents, namely 1 each from Mastomys coucha (fam. Muridae) and Helioscorus rufobrachium (fam. Sciuridae) in the Bumba zone of the Equatorial province of the Zaire Republic. Attempts to isolate the virus from 48 monkeys were negative. Neutralizing antibodies were found in 1 of 7 monkey (Cercopithecus ascanius schmidti) sera examined as well as in the serum of H. rufobrachium from which the virus was isolated.

Animals↗
PubMed1975

[Dynamics of the antismallpox antibodies detectable in the passive hemagglutination reaction with various animal immunization schemes].

Dynamics of accumulation and preservation of antibodies detectable in the PHAT (PHAT-AT) was studied on rabbits and guinea pigs with the use of various doses of the living inactivated virus and their combination in comparison with the virus-neutralizing antibodies, antihemagglutinins and precipitins. Accumulation of the virus-neutralizing antibodies did not coincide in time with the curve of the PHAT-AT accumulation; the titres of the virus-neutralizing antibodies were higher than the PHAT-AT titres. At the same time the percentage of seroconversions determined by PHAT was equal to 100 and the PHAT-AT level directly depended on the immunizing dose, the time of administration and the type of the antigen. On the basis of the data obtained PHAT could be recommended as a test for the assessment of the immunological efficacy of the smallpox vaccinations.

Animals↗
PubMedDate not supplied

[The isolation of HIV from children with the initial signs of an HIV infection and from symptom-free carriers].

A high rate of HIV carrier state was observed in seropositive children with early symptoms of HIV infection. The virus was also isolated from 2 seropositive adults (mothers) showing no clinical manifestations. The intervals of virus manifestation in culture varied from 6 to 30 days with maximal frequency of detection in the 2nd week. Different modifications of the procedure for HIV isolation were assayed, and it was shown that the efficacy of isolation (shortening of the period of virus detectability and increase in the number of the antigen-containing cells) could be improved by the addition to the culture of the Jurkat-tat III line expressing the product of the tat gene important for virus reproduction.

Adolescent↗
PubMedDate not supplied

[Results of examining wild monkeys for the presence of smallpox antibodies and smallpox group viruses].

The results of examinations of sera, blood and organs of different species of monkeys from some Asian and African countries for the presence of antibody to smallpox and viruses of the smallpox group. Significant titers of smallpox antibodies (antihemagglutinins virus-neutralizing and, in some cases, precipitating antibody) were found in a considerable number of monkeys shot near foci with human cases (Equatorial province of Zair Republic). In the same monkeys kidney tissues yielded 3 isolates of smallpox virus group two of which were indistinguishable in the laboratory tests from variola virus. On the basis of these data it is concluded that smallpox viruses circulate among wildlife monkeys in some areas of Equatorial Africa. Further studies along these lines are necessary.

Africa↗
PubMedDate not supplied

[Stability of the main components of an EIA test system for the serodiagnosis of HIV infection].

The stability of two most important components of the EIA test system for diagnosis of HIV infection, an immunosorbent (HIV antigen adsorbed on polystyrene plates) and antispecies peroxidase conjugate, was studied. The possibility to prolong the shelf life of the test system for at least up to 6 months was demonstrated. At the same time, it is proposed to supplement the existing methods of production control with the conjugate thermostability test developed by the authors who believe that this will allow to exclude conjugates with insufficient stability from the test system kit.

AIDS Serodiagnosis↗
PubMedDate not supplied

[Possible mechanism of orthopoxvirus preservation in nature].

Until recently, virus carrier state in the absence of overt clinical infection has been known for only one species of orthopoxviruses, namely, ectromelia virus. The paper describes the results of the modelling of asypmtomatic infection caused by monkeypox, cowpox, and ratpox viruses. Persistence of these viruses up to 6 weeks in animals (hamsters, cotton and white rats) with experimental asymptomatic infection as well as in apparently normal naturally infected white rats was established by isolation of virus from the organs of these animals. These results suggest that asymptomatic virus carrier state may occur both with ectromelia and a number of other orthopoxviruses. The mechanism may be of ecological importance, providing for circulation of these viruses and their preservation as biological species in nature.

Animals↗