The work deals with the comparative analysis of oxygen-dependent metabolic processes in the peritoneal macrophages of intact and immune, mice after their interaction with whole-cell pertussis vaccine (WCPV), dialyzed pertussis antigen (DPA) and fraction 2 of this antigen. The study revealed that WCPV, DPA and its fraction 2 modulated the production of active forms of oxygen by peritoneal macrophages, evaluated by means of luminol-dependent chemiluminescence (CL). The influence of WCPV of oxygen-dependent metabolic processes in macrophages after the first contacts with them had a dose-dependent character: low concentrations activated and higher concentrations suppressed the "respiratory" explosion. The immunization of animals abolished the effect of extinguishing CL on the contact of macrophages with high doses of WCPV and essentially increased the level of their CL response to all pertussis preparations in comparison with those for intact cells. DPA and its fraction 2 were not inferior to WCPV in their capacity for inducing the "respiratory" explosion, and in high doses they essentially surpassed WCPV doses in this capacity, especially on the first contact with macrophages.

Animals↗

PubMedDate not supplied

[Immune response in adult immunized with adsorbed DT-M anatoxin with reduced antigen content and Imovax-DT-adulte vaccine].

The comparative study of immune response after immunization of adults with adsorbed DT toxoid with reduced antigen content and Imovax-DT-adulte vaccine, as well as the safety of these preparations, was made. The study revealed that immunization of adults with adsorbed DT toxoid having reduced antigen content, made in two injections, and the injection of Imovax-DT-dulte vaccine, as well as the successive injection of these preparations, produced the same the levels of antitetanus immunity. Antidiphtheria immunity, evaluated by the number of seroconverted to diphtheria persons following two injections immunization was similar for the two preparations, while the level of antidiphtheria antibodies was higher in persons immunized with adsorbed DT toxoid. The immune stratum index was rather high among persons aged 16-29 years. This age group exhibited the highest number of persons, seropositive to both diphtheria and tetanus. Both vaccine preparations, adsorbed DT toxoid with reduced antigen content and Imovax-DT-adulte vaccine, were found to be equally capable of inducing autoimmune reactions in the vaccinees, detected by laboratory methods.

Adolescent↗

PubMedDate not supplied

[Characteristics of the antitoxic and antibacterial immune response in children with diphtheria].

As found in this study, the development of the manifest forms of diphtheria occurred in the presence of lower levels of antitoxic antibodies, than bacterial carrier state. The level of antitoxic antibodies in all patients, irrespective of the time of their examination, was higher than that of antibacterial antibodies. In the dynamics of the disease a considerable increase in antitoxic and antibacterial antibodies was observed in localized and disseminated forms of diphtheria and, to a considerably lesser degree, in toxic and subtoxic forms of diphtheria, while not observed in the process of the formation of bacterial carrier state. The dynamics of a rise in the levels of antitoxic and antibacterial antibodies in the manifest forms of diphtheria infection made it possible to differentiate between the cases of toxigenic Corynebacterium diphtheriae carrier state and those of the disease.

Antibodies, Bacterial↗

PubMedDate not supplied

[Antidiphtheria immunity in children after immunization with adsorbed DPT vaccine and ADPT-M toxoid].

Antidiphtheria immunity in children aged 3-15 years was evaluated, depending on their age, the vaccinal preparation chosen for immunization and the immunization schedule. Adsorbed DPT vaccine was shown to have a higher immunological activity in comparison with adsorbed DT toxoid with reduced antigen content. The relationship between the tension of antidiphtheria antitoxic immunity in children and the possibility of the formation of the clinical forms of diphtheria with different severity in the patients was established. Children aged 4-6 years were considered to be a group of high risk in diphtheria morbidity, as it was among the children of this group that toxic and subtoxic forms of diphtheria were most often registered (44.9 +/- 7.1% of the examinees); in addition, a high proportion of children seronegative to this infection (26,3 +/- 2.2%) was observed in this group.

Adolescent↗

PubMedDate not supplied

[Pathogenicity of Corynebacterium diphtheriae circulating in Rostov-on-Don City and Rostov region during interepidemic period].

Main pathogenic characteristics (toxin production, tox-gene detection, adhesiveness) of 59 strains of C. diphtheriae circulating in Rostov-on-Don city and Rostov region in 2004-2005 were studied. Study of toxigenicity of 15 tox+ C.di phtheriae strains showed full coincidence of Elek immunoprecipitation test and polymerase chain reaction (PCR). Presence of part of A-fragment of tox-gene was detected in 5 (11.4%) of 44 C. diphtheriae strains that were negative in Elek test. Hemagglutinating activity of toxin producing strains was intermediate (40%) or high (60%). Among non-toxigenic strains those with intermediate adhesiveness were predominated (45,5%), the intermediate or high adhesiveness was detected in strains positive in PCR. Obtained characteristics of C. diphtheriae can be useful for surveillance for diphtheria infection during interepidemic period.

Bacterial Adhesion↗

PubMedDate not supplied

[The potential of using low-frequency ultrasound for the correction of immunodeficiency states].

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Animals↗

PubMedDate not supplied

[Passive hemagglutination reactions in intracranial and intraspinal neoplasms].

With the use of a system of serological reactions (PHAR) on the basis of erythrocytic diagnosticums in neurosurgical patients (58 with tumors of the brain, 11 with tumors of the spinal cord, 112 with tumor-like diseases of the brain, 50 with lesions of the spinal cord marked by a tumor-like course) and in a control group of individuals (100 donors and 30 patients with tumors of the mammary gland and intestine) results were produced which bore evidence of the high specificity of the methods for detecting signs of brain damage and determining the histological nature of tumors of the brain and spinal cord in most patients. For instance, the histological nature of the tumors was identified in the preoperative period in 79% of patients with intracranial and in 73% of those with intraspinal new growths.

Autoantibodies↗

PubMedDate not supplied

[Changes in 5'-nucleotidase activity in the dynamic formation of whooping cough immunity].

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5'-Nucleotidase↗

PubMedDate not supplied

[A comparative analysis of the effect of Bordetella pertussis preparations on a primary culture of murine peritoneal macrophages].

The paper deals with comparative analysis of the effect of corpuscular pertussis vaccine (CPV), pertussis dialysate antigen (PDA) and its fraction 2 (fr. 2 PDA) on primary culture of peritoneal macrophages of mice. It is established that CPV possesses the expressed cytotoxic effect that is of dose-dependent character. PDA and 2 (fr. 2 PDA), in contrast to CPV had not such an effect. Only very high doses of preparations (100 micrograms by protein) evoked weak toxic effect on macrophages. Strict direct correlation between cytotoxic effect of pertussis preparations on the primary culture of peritoneal macrophages of mice and their capacity to induce leukolymphocytosis that permits recommending application of these cells to estimate toxicity of pertussis preparations and prediction of potentiality of development of side effects under their introduction.

Animals↗