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Biomedical subjects

E Rodríguez

Publications and source records attributed to E Rodríguez.

At least 181 records · Page 10Linked to original sources
PubMed1992

[Isolation of Vibrio cholerae no-01 in the Gulf of Nicoya, Costa Rica].

In a bacteriological study on samples of bivalves, mud and surface waters from the Gulf of Nicoya, Costa Rica, 18 strains of non-01 Vibrio cholerae and 50 of V. mimicus were isolated. The samples were enriched in alkaline peptone water, and streaked on MacConkey and inositol-brilliant green bile agars. Biochemical and serological tests were used for their identification. Both species were isolated from all sampling sites (Lepanto, Jicaral and Puntarenas) with either of the two agar media, even though these were not specific for vibrios.

Agar↗
PubMed1992

[Neurotoxicity caused by cyclosporin A in renal transplantation in children].

Cyclosporine is a specially useful immunosuppressor agent in children subjected to renal transplantation, minimizing the deleterious effect of steroids on growth and the development of Cushing syndrome. However, side effects which require close supervision are well known, including liver, kidney and central nervous system toxicity. Seizures, cerebellar ataxia, aphasia, paresthesia and behavioral disorders are characteristic of the latter. Hypertension and hypomagnesemia have been identified as risk factors. In contrast to nephrotoxicity, CNS toxicity is not related to plasma levels of cyclosporine. In this paper 2 patients, 10 and 11 year old, manifesting cyclosporine neurotoxicity after renal transplant, are reported.

Brain Ischemia↗
PubMed1992

Consistent improvement in the nutritional status of Colombian children between 1965 and 1989.

Three national anthropometric surveys carried out in Colombia in 1965-1966, 1977-1980, and 1986-1989 provide a reasonably standardized basis for comparing the nutritional status of infants and young children in those years. That comparison, presented here together with appropriate socioeconomic data, indicates marked reduction of malnutrition paralleling strong socioeconomic gains.

Adult↗
PubMed1991

[Determination of the neurotransmitters 5-hydroxytryptamine, noradrenaline, and dopamine in gastroduodenal mucosa. Clinical and histologic correlation].

We have studied the concentrations (pg/mg) of Neurotransmitters 5-hydroxytryptamine (5HT), Noradrenaline (NA) and Dopamine (DA) at mucosa normal and with different degrees of chronic inflammation mucosa of fundus (F), antrum (A) and duodenum (D) in 21 patients. We correlated the concentration of the neurotransmitters with each other, all of them in each region and with clinics, indirect of motility and histologic parameters. The average for 5HT in F: 940.2 +/- 457.4; in A: 787.5 +/- 407.0; in D: 601.6 +/- 272.0; for NA in F: 217.5 +/- 138.7; in A: 228.4 +/- 126.5; in D: 245.7 +/- 118.6 for DA in F: 50.7 +/- 32.2; in A: 46.3 +/- 31.5; in D: 53.0 +/- 45.6. The statistical analysis showed a significant correlation of DA concentration with lymphoplasmocytic infiltrated in Duodenum (r = 0,94). not any other parameter had significative relation with the neurotransmitters variations. We established that the variations of the neurotransmitter concentrations of the antrum mucosa are more associated with the duodenum than with the gastric fundus.

Adult↗
PubMed1991

[Isolation and various properties of proteinase I from the venom of the Peruvian snake Lachesis muta].

A proteolytic enzyme with high activity on casein was purified from Lachesis muta snake venom. This protein called "Proteinase I" was obtained using a gel filtration chromatography on Sephadex G-100 at pH 6.5, 0.1 M Ammonium acetate buffer, followed by ion exchange chromatography on DEAE-Cellulose at pH 7.5 and re-chromatographed on DEAE-Cellulose at pH 9.0 and 7.8 in Tris-HC1 buffer. A homogeneous band was obtained with the isolated protein on polyacrylamide gel electrophoresis. A molecular weight of 25,100 by gel filtration and an optimum pH of 8.4 were found for this enzyme. A total enzymatic activity was kept after a heating at 45 degrees C for ten minutes while the activity at 70 degrees C was 4% only. Synthetic esters as TAME and BAEE were not attacked by this enzyme. The activity was not affected by calcium ions and hemorrhagic action was not observed either.

Animals↗
PubMed1991

[First isolation of Clostridium tetani from soils of Meseta Central, Costa Rica].

Clinical evidence has long pointed to the existence of Clostridium tetani in Costa Rica. Thirty soil samples were studied for clostridia, and two yielded six strains of C. tetani, four of which proved to be toxigenic when mice were inoculated intraperitoneally with the culture supernates. These four isolates could be neutralized when their toxic supernates were admixed with tetanus antitoxin.

Clostridium tetani↗
PubMed1990

Detection of cows' milk in ewes' milk and cheese by an indirect enzyme-linked immunosorbent assay (ELISA).

An indirect enzyme-linked immunosorbent assay was successfully developed for the detection of defined amounts of cows' milk (1-50%) in sheeps' milk and cheese. The assay used polyclonal antibodies raised in rabbits against bovine caseins (BC). The anti-BC antibodies were recovered from the crude antiserum by immunoadsorption and elution from a column containing BC. The antibodies were biotinylated and rendered cows' milk specific by mixing them with lyophilized ovine and caprine caseins. ExtrAvidin-peroxidase was used to detect the biotinylated anti-BC antibodies bound to BC immobilized on 96-well plates. Subsequent enzymic conversion of substrate gave clear absorbance differences when assaying mixtures of sheeps' milk and cheese containing variable amounts of cows' milk.

Animals↗
PubMed1990

Pseudotumor cerebri complicating typhoid fever.

The case of a 22-year-old patient is described, who developed a clinical picture of pseudotumor cerebri in the course of typhoid fever, an association not previously described. The patient progressed favorably after treatment with amoxicillin. The etiopathogenesis of pseudotumor cerebri is briefly discussed, as is the need to study the possible existence of this disorder in cases of typhoid fever patients with headaches and/or visual deficits which are not readily explained.

Adult↗
PubMed1990

[A comparative study of myelography and CAT in patients with lumbo-sciatica].

One hundred patients with acute lumbo sciatica symptoms probably due to disc hernia, have been studied. A CT scan and a myelography was performed in all patients. The clinical and radiologic (myelography and CT scan) findings have been evaluated in three categories based on their intensity and a correlation between these three parameters has been performed. Our results show a positivity of 94% in the clinical signs, 87% in the myelography findings and 88% in CT scan findings. The correlation results show that in very severe lumbo sciatica syndromes there is a greater positivity in the myelography than in CT scan findings and that there could be big radiologic changes without a clear clinical repercussion.

Acute Disease↗
PubMed1990

[Right ventricular function and effort tolerance in patients with chronic congestive heart insufficiency. A cross-over double-blind study].

We tried to assess the value of both ventricular function changes and its correlation with maximal exercise capacity in patients with chronic heart failure. For this purpose, a double blind crossover study was designed, and the change in the exercise tolerance and both ventricular ejection fraction were evaluated. When compared with digoxin treatment (p less than 0.01) and with a control-period (p less than 0.001), the captopril increases total exercise time significantly. The response of right ventricular ejection fraction was similar. The changes in right ventricular ejection fraction, but not those of left ventricular ejection fraction, correlated with the variations of exercise time (r = 0.67). These facts suggest that right ventricular function is an important determinant of exercise capacity in patients with chronic heart failure and that its behaviour explain, in part, the response to captopril treatment.

Adult↗
PubMed1989

Development of a cows' milk identification test (COMIT) for field use.

A cows' milk identification test (COMIT) has been successfully developed for the detection of defined amounts of cows' milk (3-100%) in ewes' milk. The assay uses polyclonal antibodies raised in goats against bovine whey proteins. The test, an agar-gel immunodiffusion technique, uses agar plates with a printed template for correct placement of the test components (antisera discs, positive and negative milk reference discs and sample discs). The presence of cows' milk in the sample was recorded as positive when the precipitin lines appearing between the sample disc and the antisera disc fuse completely with those from the positive cows' milk reference disc. This test is reliable, practical and easy to perform in the field and in dairy factories. Furthermore, the nature and stability of the components of the test make it suitable for commercial development into kits which should be highly practical in any kind of inspection programme concerned with milk species identification.

Animals↗