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E Romanowska

Publications and source records attributed to E Romanowska.

At least 37 records · Page 2Linked to original sources
PubMed1995

Structural elucidation of the biological repeating unit of O-specific polysaccharide from Citrobacter serotype O41.

Sugar analysis of the O-specific polysaccharide produced by Citrobacter serotype O41 revealed the presence of a hexasaccharide repeating unit, which includes the unusual 3-amino-N-(D-3'-hydroxybutyryl)-3,6-dideoxy-D-galactosyl residue (Fuc3NAcyl). The structure of the repeating unit was determined by extensive use of homo- and heteronuclear two-dimensional NMR spectroscopy, including the application of long-range 1H-13C correlation experiments and NOE studies to establish the sequence of sugar units. Indentification of the Glc beta 1-->2Fuc3NAcyl beta 1-->6GlcNAc alpha 1-->sequence at the non-reducing terminus establishes the biological repeating unit of this O-specific polysaccharide as: -->2Glc beta 1-->Fuc3NAcyl-beta 1-->6GlcNAc alpha 1-->(Glc alpha 1-->2) 4Gal beta 1-->3GalNAc beta 1-->. This structure is similar to that found for the O-specific polysaccharide isolated from Hafnia alvei strain 1211 [Katzenellenbogen, E., Romanowska, E., Dabrowski, U. & Dabrowski, J. (1991) Eur. J. Biochem. 200, 401-407], which differs in having an acetyl substituent at O4 of the Fuc3NAcyl residue and a branch point of GalNAc alpha substituted by Glc beta at O3; these differences are responsible for the only weak serological cross-reactivity of the two strains.

Carbohydrate Sequence↗
PubMed1995

3-Deoxy-octulosonic-acid-containing hexasaccharide fragment of unusual core type isolated from Hafnia alvei 2 lipopolysaccharide.

The hexasaccharide containing 3-deoxy-octulosonic acid (Kdo) was isolated from the carbohydrate material obtained after the mild acid hydrolysis of lipopolysaccharide of Hafnia alvei strain 2. The hexasaccharide was purified by gel filtration on Bio-Gel P-4 and P-2 columns. On the basis of sugar and methylation analyses, one- and two-dimensional NMR spectroscopic methods, the hexasaccharide was identified as [formula: see text] The tetrasaccharide linked to position 7 of Kdo is also part of the sialic-acid-containing O-specific unit. Di-substitution of Kdo at positions 7 and 8 has not been previously reported.

Carbohydrate Conformation↗
PubMed1995

Lipopolysaccharide core region of Hafnia alvei: structure elucidation using chemical methods, gas chromatography-mass spectrometry, and NMR spectroscopy.

Sugar and methylation analysis with the use of gas chromatography-mass spectrometry and 1H NMR spectroscopy proved that the core oligosaccharides isolated from lipopolysaccharides of eight Hafnia alvei strains have the identical hexasaccharide skeleton. However, 1H, 31P heterocorrelated spectra showed that the phosphorylation pattern is not the same. The branched heptose for the ATCC 13337, 1187, 2, 1191, 1196, 1220, and 481L strains is phosphorylated as in the following formula, where P = -O-P(O)(O-)2 and P-PEtN = [-O-P(O)(O-)]2-O(CH2)2NH3+ [formula: see text] A different phosphorylation pattern was found for the 1211 strain, where the branched heptose residue is 6-substituted by a monophosphorylethanolamine group, ...-->3(-->7)(PEtN-->6)-alpha-LD-Hepp-(1-->3)..., where PEtN = -O-P(O)(O-)-O(CH2)2NH3+.

Carbohydrate Sequence↗
PubMed1995

Lipopolysaccharide core region of Hafnia alvei: serological characterization.

Covalent glycoconjugates containing, as a ligand lipopolysaccharide core, oligosaccharides of Hafnia alvei standard strain ATCC 13337 and R mutant 1 M were used to produce anti-H. alvei core antibodies. The sera obtained were tested in rocket immunoelectrophoresis, immunoblotting and ELISA using H. alvei lipopolysaccharides of various strains. The experiments were carried out to study the antigenic relationships between lipopolysaccharide core regions in the H. alvei genus.

Animals↗
PubMed1994

The structure of the O-specific polysaccharide of Hafnia alvei strain 1216.

The O-specific polysaccharide of Hafnia alvei strain 1216 is composed of D-galactose, D-glucuronic acid, 2-acetamido-2-deoxy-D-glucose, 3,6-dideoxy-3-[(R)-3-hydroxybutyramido]-D-glucose, and O-acetyl groups in the ratios 1:1:2:1:1. On the basis of sugar and methylation analyses of the intact and chemically degraded (O-deacetylated, carboxyl-reduced, Smith-degraded) polysaccharide and 1H and 13C NMR spectroscopy, including 2D shift-correlated (COSY, relayed COSY, 13C, 1H-COSY) and 1D NOE spectroscopy, it was concluded that the O-antigen is built up of linear pentasaccharide units having the following structure: [formula: see text]

Carbohydrate Conformation↗
PubMed1994

Evidence for two different Rieske iron-sulfur proteins in the cytochrome bf complex of spinach chloroplast.

Spinach thylakoids, grana vesicles, stroma lamellae vesicles and also isolated cytochrome bf complex, were analysed by two-dimensional polyacrylamide gel electrophoresis employing isoelectric focusing in the first dimension and sodium dodecyl-sulfate polyacrylamide gel electrophoresis in the second dimension. About 100 thylakoid membrane proteins were resolved. In all cases the Rieske FeS protein separated into two polypeptide spots having the isoelectric points of 5.1 and 5.4, respectively. The Rieske FeS protein was identified by immunoblot analysis and by microsequences of the first 23 N-terminal amino acids. The intensity of the Coomassie brilliant blue stain of the two spots was stronger for the Rieske FeS protein of the grana vesicles than for that of the stroma lamellae vesicles.

Amino Acid Sequence↗
PubMed1994

Serological and structural features of Hafnia alvei lipopolysaccharides containing D-3-hydroxybutyric acid.

The serological heterogeneity of Hafnia alvei lipopolysaccharides from strains ATCC 13337, 1187, 1221, 114/60, 1211 and 1216, that contain D-3-hydroxybutyric acid, was analyzed by rocket immunoelectrophoresis, immunoblotting and passive hemagglutination. The significance of D-3-hydroxybutyric acid component for their cross-reactivity has been discussed. The results obtained allowed us to place four H. alvei strains (ATCC 13337, 1187, 1221 and 114/60) in one serotype (A) and to consider two other strains (1211 and 1216) as separate serotypes (B and C, respectively).

3-Hydroxybutyric Acid↗
PubMed1993

3-Deoxy-octulosonic acid-containing trisaccharide fragment of an unusual core type of some Hafnia alvei lipopolysaccharides.

The 3-deoxy-octulosonic acid-containing trisaccharide was isolated from the carbohydrate material obtained after the acid hydrolysis of lipopolysaccharides of Hafnia alvei strains 2 and 1211 using gel filtration. For purification the trisaccharide fraction was rechromatographed on a Bio-Gel P2 column. On the basis of sugar and methylation analyses and 1H NMR spectroscopy the trisaccharide was identified as 3-deoxy-7-O-(6-O-acetyl-alpha-D-galactopyranosyl)-4-O-(alpha-L-glycero-D - mannoheptopyranosyl)-D-manno-2-octulopyranosic acid. The occurrence of such bifurcate Kdo-containing structure has not been previously reported in Enterobacteriaceae.

Carbohydrate Sequence↗
PubMed1993

Structure of the O-specific polysaccharide containing pentitol phosphate, isolated from Hafnia alvei strain PCM 1191 lipopolysaccharide.

The O-specific polysaccharide of the lipopolysaccharide produced by Hafnia alvei strain PCM 1191 was shown by composition and methylation analyses, periodate oxidation and one- and two-dimensional 1H- and 31P-NMR spectroscopy to be a polymer of a branched hexasaccharide repeating units having the structure: [formula: see text] where LAraol = L-arabitol The polysaccharide of 1191 strain has teichoic-acid-like character. Its peculiar feature is the presence of arabitol phosphate, a component observed for the first time in bacterial lipopolysaccharides.

Carbohydrate Conformation↗
PubMed1992

The structure of the sialic acid-containing Escherichia coli O104 O-specific polysaccharide and its linkage to the core region in lipopolysaccharide.

Mild acid hydrolysis of Escherichia coli O104 lipopolysaccharide released an O-specific polysaccharide, a tetrasaccharide repeating unit, the corresponding dimer, and a disaccharide fragment of the repeating unit. Complete and incomplete cores, and oligosaccharides comprising fragments of the repeating unit and the core region, were also obtained. On the basis of sugar and methylation analysis, FAB-mass spectrometry and NMR spectroscopy of the hydrolysis products, the repeating unit of the O-specific polysaccharide was shown to be the tetrasaccharide:-->4)-alpha-D-Galp-(1-->4)-alpha-Neup5,7,9Ac3++ +-(2-->3)-beta-D- Galp-(1-->3)-beta-D-GalpNAc (1-->. The linkage between the O-specific polysaccharide chain and the core region, which appeared to be of the R2 type, was established. These results indicate that N-acetylneuraminic acid, located in the O-specific polysaccharide, is an inherent lipopolysaccharide component.

Carbohydrate Conformation↗
PubMed1992

The structure of a glycerol teichoic acid-like O-specific polysaccharide of Hafnia alvei 1205.

The O-specific polysaccharide of Hafnia alvei 1205 contained D-glucose, D-galactose, 2-acetamido-2-deoxy-D-glucose, 4-acetamido-4,6-dideoxy-D-glucose (Qui4NAc), glycerol, phosphate, and O-acetyl groups. On the basis of 1D and 2D shift-correlated homonuclear and 13C-1H heteronuclear NMR spectroscopy, methylation analysis, Smith degradation, and dephosphorylation with hydrofluoric acid, it was concluded that the O-antigen was a partially O-acetylated teichoic acid-like polysaccharide having the following structure: [formula: see text]

Carbohydrate Conformation↗
PubMed1992

Immunochemical characterization of Citrobacter strain PCM 1487 O-specific polysaccharide- and core oligosaccharide-protein conjugates.

O-specific-polysaccharide and core oligosaccharide were isolated from Citrobacter strain PCM 1487 lipopolysaccharide and purified. The polysaccharide was selectively devoid of 4-deoxy-D-arabinohexose residues. Covalent conjugates of the modified O-specific polysaccharide and of the core oligosaccharide with tetanus toxoid were prepared. Immunochemical characterization of these conjugates proved that they are strong immunogens. Using monospecific rabbit antisera raised against the conjugates, the antigenic relationships between lipopolysaccharides of various strains of Citrobacter, Shigella sonnei, and Shigella flexneri were studied by quantitative microprecipitin and quantitative microprecipitin inhibition and immunoblotting tests.

Bacterial Proteins↗
PubMed1992

Immunochemical studies on sialic acid-containing lipopolysaccharides from enterobacterial species.

Lipopolysaccharides with sialic acid as a component were isolated from six bacterial serovars of the family Enterobacteriaceae. SDS-polyacrylamide gel electrophoresis, immunoblotting and passive haemagglutination demonstrated that epitopes are localized in the O-specific polysaccharide of lipopolysaccharides. The strains were tested for a serological relationship; the only distinct cross-reactivity recorded was between Escherichia coli serovars O24 and O56. The molecular mimicry phenomenon was found for Citrobacter freundii serovar O37 which shared epitopes with horse as well as human red blood cells.

Animals↗
PubMed1991

O-specific polysaccharide of Hafnia alvei lipopolysaccharide isolated from strain 1211. Structural study using chemical methods, gas-liquid chromatography/mass spectrometry and NMR spectroscopy.

The Hafnia alvei strain 1211 O-specific polysaccharide is composed of 3-amino-N-(D-3'-hydroxybutyryl)-3,6-dideoxy-D-galactose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine and D-glucose (1:1:2:2). On the basis of sugar and methylation analyses, Smith degradation, and one- and two-dimensional 1H- and 13C-NMR spectroscopy, the polysaccharide was shown to be an O-acetylated polymer of the repeating hexasaccharide unit, ----2D(4-OAc)Fucp3NAcyl beta 1----6DGlcpNAc alpha 1---- (DGlcp beta 1----3)4DGalpNAc alpha 1----3DGlcpNAc beta 1----2DGlcp beta 1----, where DFucp3NAcyl = 3-amino-N-(D-3'-hydroxybutyryl)-3,6-dideoxy-D- galactopyranose. The O-specific polysaccharide showed some microheterogeneity due to incomplete substitution by terminal glucose.

Acetylgalactosamine↗
PubMed1991

Structure of the O-specific, sialic acid containing polysaccharide chain and its linkage to the core region in lipopolysaccharide from Hafnia alvei strain 2 as elucidated by chemical methods, gas-liquid chromatography/mass spectrometry, and 1H NMR spectroscopy.

Mild acid hydrolysis of Hafnia alvei strain 2 lipopolysaccharide released no O-specific polysaccharide but instead gave a monomeric octasaccharide repeating unit with N-acetylneuraminic acid as the reducing terminus. In addition, a dimer of the octasaccharide repeating unit, and also a decasaccharide composed of a fragment of the O-specific polysaccharide chain and the core region, were obtained in minute amounts. On the basis of the sugar and methylation analyses, periodate oxidation, and 1H NMR spectroscopy of the lipopolysaccharide hydrolytic products, the biological repeating unit of the O-specific polysaccharide was shown to be a branched octasaccharide: (Formula; see text) The linkage between the O-specific polysaccharide chain and core region has also been determined and has yield strong evidence that N-acetylneuraminic acid is an inherent lipopolysaccharide component. The lipopolysaccharide of H. alvei strain 2 is the first lipopolysaccharide reported to contain 4-substituted neuraminic acid in its O-specific polysaccharide region.

Carbohydrate Conformation↗