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Biomedical subjects

E Sawicka

Publications and source records attributed to E Sawicka.

At least 37 records · Page 2Linked to original sources
PubMed1977

The ultrahistochemical picture of the so-called reversed ATPase in the gastrocnemius muscle of the rat.

The ultrahistochemical localization of the "reversed" ATPase activity was investigated. Red muscle fibres showed permanent sarcomere contraction, enzymatic activity in the inner membrane and matrix of mitochondria, and large, osmiophilic, probably calcium-containing structures within mitochondria and on their outside. White muscle fibre sarcomeres were relaxed, and activity within their sarcoplasmic reticulum was marked, but slight in the mitochondria. The relaxed state of the sarcomere in the white muscle fibres is supposed to be connected with inactivation of myofibrillar ATPase by acid preincubation, whereas red muscle contraction indicates that acid preincubation does not inactivate their myofibrillar ATPase. That the product of its activity failed to become visible in the sarcomeres is probably due to imperfection of the method. Two sub-types of red muscle fibres were distinguished: those showing only enzymatic activity in mitochondria, and those containing large intra- and extramitochondrial osmiophilic structures. The origin and composition of these structures is difficult to explain. A relation seems to exist between their presence within mitochondria and outside.

Actomyosin↗
PubMedDate not supplied

[Preoperative diagnosis and surgical treatment tactics for anorectal malformations in light of personal observations].

The article presents contemporary views on classification, diagnostics and surgical treatment of congenital anorectal anomalies. The model of one stage or multi stage repair depending on the kind of defect and the necessity the anal sphincter muscles reconstruction was discussed. It was noticed that the most important independent factor which can have influence on the functional outcome is a normally developed sacral bone. The objective method of evaluation of the sacrum proposed by Schneider Hospital in New York is presented.

Abnormalities, Multiple↗
PubMedDate not supplied

[Advances in treatment of congenital gastroschisis based on personal examinations].

The aim of the study was the definition of standard approach to a newborn with gastroschisis. The rules of treatment introduced in 1992 enabled the closure of the abdominal wall primarily in all newborns and made it possible to decrease the mortality considerably. This was achieved despite the fact that we usually did not have influence on time and method of delivery or quality of transportation to our department. In the Department of Pediatric Surgery of Institute Mother and Child, in the years 1992-1997, twenty three newborns with gastroschisis were admitted. The standard approach in all patients was the following: insertion of the catheters to the small bowel through the appenddicocaecostomy and to the large bowel through the rectum, intraoperative irrigation of the meconium (mucosolvan), forceful stretching of the abdominal wall, returning of extraabdominal viscera under control of central venous pressure or middle airway pressure (anesthesiologic control). In the postoperative period all patients routinely had controlled ventilation, administration of analgesic drugs, catecholamines, antibiotics and total parenteral nutrition. Intestinal catheters were gradually removed every day for irrigations of all levels. Oral nutrition was started after the return of effective peristalsis. From 23 treated newborns 16 are alive. 7 children died, 4 of them were resuscitated before operation because of severe general condition. Two patients died because of sepsis in the second month of life, one because of SIDS (sudden infant death syndrome) in the third week of life. Sixteen children are followed up. The development of all children is normal.

Abdominal Muscles↗
PubMedDate not supplied

[Congenital jejunoileal and colonic atresia].

Jejunoileal and colonic atresias are a common cause of neonatal intestinal obstruction. Authors present the aetiology, types of atresias, clinical symptoms, diagnostics, surgical treatment, problems of postoperative management and prognosis in the newborns with congenital intestinal atresia. In the years 1992-2000, in the Department of Paediatric Surgery and in the Department of Pathology and Intensive Care of Newborn at the National Research Institute of Mother and Child in Warsaw, 539 newborns were surgically treated (congenital anomalies of gastrointestinal tract, genito-urinary system, central nervous system, defects of abdominal wall and diaphragm). In 21 patients jejunoileal or colonic atresia were confirmed. On the base of the course of treatment the authors analyse prognostic factors: prematurity, certain types of atresia, additional pathology accompanying intestinal defect, necessesity of re-section of the long part of the bowel or multiple anastomosis. Diagnostic and treatment problems in three patients with short bowel syndrome needed additional discussion.

Colon↗
PubMedDate not supplied

Muscular fatigability in mitochondrial myopathies. An electrophysiological study.

A frequent occurrence of ophthalmoplegia and muscle fatigability in mitochondrial myopathy (MAM) often makes its differential diagnosis from myasthenia rather difficult. Neuromuscular transmission was investigated in 9 patients with MAM, presenting marked fatigability. The aim of the study was to see whether there were any other causes of muscle fatigability in addition to the metabolic factors. Classical electrostimulation as well as the SFEMG, which is very sensitive in detecting neuromuscular transmission disorders, were used. The findings were far from uniform: we found normal neuromuscular transmission in 5 cases, in 3 patients we observed slight abnormalities of neuromuscular transmission, in 1 case neuromuscular transmission disturbances seemed to be of neurogenic origin. Our results allow an assumption that the causes of muscle fatigability in MAM are of a much more complex nature than it has been anticipated. They might depend not only on the metabolic disorders within the muscle fibre itself but also on the impaired function of the peripheral nerve or of the neuromuscular junction. All the mechanisms combined may also play a role, though in individual patients the contribution of particular factors responsible may vary.

Adolescent↗
PubMedDate not supplied

Primary systemic carnitine deficiency under successful therapy: clinical, biochemical, ultrahistochemical and renal clearance studies.

Systemic carnitine deficiency is an often fatal, but treatable metabolic disorder which should be considered in any child with repeated episodes of a Reye-like syndrome or a cardiomyopathy. A 4-year-old girl with a typical history and clinical findings was successfully treated with oral carnitine. Despite low liver carnitine, ketogenesis upon fasting was normal. Normal muscle function under therapy was associated with unchanged low muscle carnitine levels. Improvement of mitochondrial structure and function was demonstrated by controlled ultrahistochemical studies. A renal carnitine leak, evident from renal clearance studies, may contribute to the pathogenesis of systemic carnitine deficiency.

Biopsy↗