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Biomedical subjects

E Strobel

Publications and source records attributed to E Strobel.

At least 73 records · Page 4Linked to original sources
PubMed1988

A new second-generation anti-HIV-1 enzyme immunoassay using recombinant envelope and core proteins.

In a multicenter collaborative study a new second-generation HIV-1 antibody enzyme immunoassay (Abbott recombinant HIV-1 EIA) using Escherichia coli-expressed recombinant p24 and p41 proteins as solid-phase antigens was compared with the first-generation H9 cell-line-based Abbott HIV-1 EIA. The results of the confirmatory assays (Western blot, immunofluorescence), combined with clinical information, were used as the reference standard for the detection of HIV-1 antibodies in 10,676 random blood donor serum specimens, in a panel of 840 specimens from symptomatic and asymptomatic patients and a total of 63 serial blood specimens from 23 people at risk. With fresh blood donor sera, the specificity of the first-generation assay ranged between 99.54 and 99.76% (95% confidence limits, CL) compared with 99.81-99.95% (95% CL) for the second-generation EIA. With panel specimens the recombinant HIV-1 EIA achieved an overall sensitivity of 100% and a specificity range of 98.3-99.7% (95% CL); the corresponding sensitivity and specificity ranges observed for the first-generation EIA were 98.0-99.5% (95% CL) and 94.3-96.8% (95% CL), respectively. The improved sensitivity for the second-generation assay was confirmed by testing serial samples from seroconverting patients. The use of recombinant proteins eliminated non-specific reactions due to class II human leukocyte antigen (HLA)-directed antibodies.

Blood Donors↗
PubMed1988

[The biological monitoring of ecologic cadmium exposure in the free state of Bavaria].

In 1984/1985 631 blood-donors settled in different regions of Bavaria took part in a "cadmium-campagne". Due to this project a substantial medical anamnesis was made and blood has been investigated to determine the cadmium level, furthermore, urine was taken for the determination of cadmium, total protein, beta 2-microglobulin, creatinine and density. In conformity with recent epidemiological investigations in Europe it was evident that the actual internal cadmium exposure - in relation to the cadmium level in blood - has not increased. Subsequently a not acceptable accumulation was excluded accordingly to the result of the proofed renal excretion of cadmium. In consideration of the established low cadmium level in biological material there was no impairment of the kidneys due to cadmium. This is also obvious in the total protein- and beta 2-microglobulin-excretions in urine which are in the normal range. By dividing the collective according to smoking habits this study confirmed, that higher values have been linked with the smokers. However, as to the age and sex there were no significant influences. The evaluation of a regional subdivision of the received samples in overcrowded regions, rural and areas far from industry did not show notable differences. Furthermore, a differentiated aspect was seen after the classification into domiciles, whereby 8 different cities, small towns and villages, resp., were considered. Despite of various differences it must be pointed out, that the cadmium level in blood as well as in urine is below the normal levels with some exceptions only. This study did not show criteria for a risk caused by cadmium which effects the general population of Bavaria.

Adult↗
PubMed1987

[Therapeutic lymphapheresis in leukostasis-induced priapism].

Priapism occurred as a result of leukostasis due to an extreme rise in leukocyte count (503,000/microliter, 96% lymphocytes) in a 46-year-old man with chronic lymphatic leukaemia. In addition to surgical treatment, anticoagulant administration and cytostatic treatment several courses of lymphapheresis were performed using a cell separator. This latter procedure quickly reduced the number of lymphocytes and improved the patient's operability.

Combined Modality Therapy↗
PubMed1986

Acquired immune haemolysis by anti A 1 antibody following bone marrow transplantation.

In ABO mismatched organ or bone marrow transplants recently some cases of acquired immune hemolysis have been reported. It was felt that these life threatening complications were due to immunosuppressive treatment with cyclosporin-A. A case of severe hemolysis following mismatched BMT is reported. Here no cyclosporin-A treatment was given since the bone marrow was T-cell deprived by an E-rosetting technique. Apparently T-cell purging can under these conditions become dangerous.

ABO Blood-Group System↗
PubMed1985

[Stromal endometriosis. A rare semimalignant tumor with a tendency to recur. Clinical follow-up over 17 years].

The authors report on a female patient with stromal endometriosis. Clinical observation at the authors' clinic extended over an unusually long period, i.e., 17 years. After describing the histology, clinical symptoms, and therapeutic possibilities, the disease course, the repeated operations necessary, and the histological findings are presented in detail. In spite of the rarity of stromatosis it should be included in the differential diagnosis when diagnosing uterine sarcoma. After histological confirmation and the completion of surgical treatment of a stromatosis, recurrence and malignancy must be considered as possibilities even many years later.

Adult↗
PubMed1984

In vitro packaging of mature phage DNA by Salmonella phage P22.

Mature, headful-sized DNA extracted from the Salmonella phages P22 and L, and P22/L-hybrid phages can be encapsulated in vitro by means of a packaging system for exogenous DNA. The probability of packaging reaches about 10(-3) per headful-sized molecule. The absence of in vitro recombination was demonstrated, to eliminate the possibility that such a process had created concatemers. The endonucleolytic cut at the pac site, which initiates sequential packaging in vivo, does not occur with the mature DNA substrate in vitro. The position of pac on the molecule is not important but the pac-recognizing phage protein gp3 is indispensable for in vitro encapsulation.

DNA, Viral↗
PubMed1984

mu-2: mutator gene in Drosophila that potentiates the induction of terminal deficiencies.

An x-ray-dependent mutator on chromosome 3 of Drosophila melanogaster is described that specifically increases the recovery of deletions for chromosomal tip regions. Such deficiencies can be induced on any chromosome. More centromere proximal mutations, as assayed by the sex-linked recessive lethal test, are not increased over the wild-type control. As far as can be determined by genetic, cytological, and molecular assays, the deletions extend to the very end of the chromosome involved. In addition, the frequency of these deletions is directly proportional to x-ray dose, suggesting that they are one-break rearrangements. It is proposed that the mutator is blocked in a major pathway for the repair of DNA double-strand breaks, and that a minor repair pathway is responsible for the addition of new telomeres under these conditions.

Animals↗
PubMed1982

Mobile dispersed repeated DNA elements in the Drosophila genome.

The molecular and cytogenetic organizations of 19 nonhomologous dispersed repeated sequence families were studied in 15 different laboratory strains of Drosophila melanogaster. Elements from each of the families appear to undergo transposition within the Drosophila genome, because there were striking differences in both the number and chromosomal locations of these elements between strains. A significant fraction (greater than 1%) of Drosophila DNA therefore has an unstable genomic organization. Each middle repetitive family exhibited similar variations in the chromosomal distribution of elements between the strains. Although the movements of these elements are not limited to a small number of genomic sites, there are chromosomal regions where elements from the different dispersed repeated DNA families appear to be clustered. The locations of such preferred integration sites are different in each of the D. melanogaster strains examined.

Base Sequence↗
PubMed1980

[Intra-uterine fetal death following motor vehicle accident (author's transl)].

Following an ultra-sound examination in the prenatal clinic as an out-patient the 27 year old gravida: 2 para; 1 sustained severe blunt trauma to the abdomen and a fracture of the pelvis and a cerebral concusion in a motor vehicle accident at 25 weeks gestation. Following admission for the motor vehicle accident the intra-uterine fetal death was shown. The patient required laparotomy with spleenectomy and suturing of ruptured liver and ruptured mesentery. The 500 g. fresh stillbirth was delivered by hysterotomy. No placental or fetal trauma to explain the intra-uterine death was found although there was vaginal bleeding on admission.

Accidents, Traffic↗
PubMed1979

Polymorphisms in the chromosomal locations of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila.

The number and chromosomal locations of elements of the 412, copia and 297 dispersed repeated gene families differ extensively when the genomes of four D. melanogaster strains are compared. Differences among individuals from the same laboratory stock in the arrangement of these elements are also observed. In contrast to these polymorphisms, the structures of the elements themselves are closely conserved. Our results indicate that 412, copia and 297 are capable of evolutionarily rapid transpositions to new chromosomal sites.

Alleles↗
PubMed1979

In vitro packaging of exogenous DNA by Salmonella phage P22.

We describe in vitro conditions for packaging of exogenous DNA of Salmonella phage P22 which has terminally redundant, circularly permuted DNA. The method is a modification of the Kaiser-Masuda procedure. The most important aspect is to prepare all components (proheads, enzymes and concatemeric DNA) in end- cells. The influence of several factors such as DNA- and Mg2+ concentration and kinetics has been investigated.

Adenosine Triphosphate↗
PubMed1978

Incomplete dosage compensation in an evolving Drosophila sex chromosome.

Cellular autoradiography was used to measure relative rates of chromosomal RNA synthesis and to examine the regulatory phenomenon of X-linked dosage compensation in Drosophila miranda, a species containing two distinct, nonhomologous X chromosomes (X1 and X2). The X1 chromosome was found to be dosage-compensated, since the rate of RNA synthesis along the single X1 chromosome in males equaled that of both X1 chromosomes in females. Unlike other sex chromosomes that have been studied, the more recently evolved X2 heterochromosome exhibited regional differences in transcriptional activity when males and females were compared. The distal 10% of the X2 was not dosage-compensated, whereas the majority of an interior segment, representing 30% of the X2 chromosome's length, was found to be dosage-compensated. Our data are consistent with the idea that the evolution of X2 dosage compensation has paralleled the differentiation of the X2 sex chromosome. In addition, gene rearrangement seems to have accompanied the acquisition of a dosage-compensory mechanism in the X2.

Animals↗
PubMed1975

[Participation of foreign workers in prenatal care].

On admission for delivery of foreign patients no or only few results of routine examinations during prenatal visits are available. Therefore the participation of foreign employees in prenatal care was reviewed. Of the 1,383 births in our clinic during 1972 there were 214 foreign mothers, 206 of them could be included in this investigation. Results show that quantity and quality of prenatal routine examinations were clearly not sufficient and adequate compared to German patients.

Female↗