Biomedical subjects

E Vax

Publications and source records attributed to E Vax.

9 recordsLinked to original sources

PubMed2003

Performance and bone development in broiler chickens given 25-hydroxycholecalciferol.

1. A series of 5 trials was conducted with Cobb chickens in order to determine the effect of 25-hydroxycholecalciferol (25OHD3) on their performance and bone development under adequate Calcium (Ca) and Phosphorus (P) supplementation, and under moderate dietary restriction of Ca and P. Formulated beadlets of 25OHD3, trade name HY-D (IsoGen, Naperville, IL, USA) were used as the 25OHD3 source. 2. Five to 10 microg of cholecalciferol (vitamin D3) or 25OHD3/kg diet were sufficient to ensure normal body weight (BW) and bone ash in chickens under continuous lighting. The two materials had similar effects on BW and bone ash. 3. In one out of the three experiments, 25OHD3 increased BW and BW gain, while in the others it had a similar effect to that of vitamin D3, or even a slight negative effect in a trial conducted on the floor, in which the diets were supplemented with the D sources at 75 microg/kg. The effects of both D sources on bone ash and on the severity or frequency of tibial dischondroplasia were similar. 4. 25OHD3 restrained the effect of moderate dietary P restriction, but not of Ca restriction, on BW gain and bone ash in 22-d-old chickens. This effect could not be explained by an higher P bioavailability in the 25OHD3-fed chickens.

Animal Feed↗

PubMed2003

Alternative procedures for molt induction: practical aspects.

Shortening daylight (to 10.5 to 11 h/d) slightly reduced the rest period (the interval between last egg and first clutch), whereas feeding a low-protein diet during the 22-d period following 8 d of feed withdrawal (FW) (recovery period) markedly extended it. Feed withdrawal accompanied by a short-daylight regime and a long recovery period led to the best postmolt production. However, production per hen housed during the whole experimental period was only slightly increased. Thus, a long rest period may mask the positive effects of short daylight and recovery diet. At least 140 to 170 d were needed to enable the molted hens to compensate for the loss of eggs during the rest period. Therefore, under certain economic conditions, rearing of nonmolting hens for 640 to 700 d should be an economic consideration. Ten days of feeding of a diet containing 0.06% nicarbazin (NICR) arrested egg production and caused a 22-d rest period but not a typical molt. Few variables of production or shell quality were improved by NICR but to a lesser extent than by FW or Zn feeding. Five days of feeding on a high-Zn diet (20 g Zn/kg; HZn) improved postmolt performances similarly to FW. Ten days of feeding on a modest-Zn (2.8 g Zn/kg), low-Ca, and low-P diet (Zn-CaP) affected postmolt performances inconsistently. In one out of two trials (trial 2), the effects of Zn-CaP were similar to those of FW or HZn; in the other (trial 3), the effects were less pronounced, more time was required for egg arrest, and more eggs were laid occasionally during the rest period. In trial 2, only the Zn-CaP diet was accompanied by short daylight. In both trials, feed intake during the induction period was only slightly reduced. Zn feeding increased the yolk Zn content slightly in eggs laid during the induction period and at the onset of production. In trial 2, only Zn-CaP markedly increased yolk Zn of eggs laid during the first 5 d of production.

Animal Feed↗

PubMed2002

Re-evaluation of calcium and phosphorus requirements in aged laying hens.

1. A series of 5 trials was conducted with 401- to 650-d-old Lohmann, Yafa (local breed with brown eggshells) and Yarkon (local breed with white eggshells) hens fed for 56 to 84 d with diets containing 25 to 50 g/kg calcium (Ca) and 4.5 to 7.6 g/kg phosphorus (P). 2. Increasing dietary Ca from 24-25 to 36-40 g/kg improved egg production, shell weight (SW) and shell thickness (ST), and decreased mortality. 3. Increasing dietary Ca to 48 to 50 g/kg did not affect egg production but increased SW and/or ST. In one out of the 4 trials, this effect was not significant, most likely because of the high shell quality of the eggs from the Yafa hens used for this trial. 4. Dietary P content of 4.5 g/kg (1.0 g/kg added inorganic P) appears to be sufficient for maintaining egg production and shell quality in aged laying hen given 36 to 40 g/kg Ca. 5. Increasing dietary Ca above 40 g/kg may require a higher dietary P content.

Aging↗

PubMed2001

Air velocity alters broiler performance under harsh environmental conditions.

The effect of air velocity (AV) on weight gain, feed intake, and feed efficiency was studied in male broiler chickens exposed to a constant 35 C, 60% RH, and continuous illumination from 4 through 7 wk of age. In two separate trials, AV of 0.5, 1.5, 2.0, and 3.0 m/s (Trial 1) and 1.0, 1.5, 2.0, and 2.5 m/s (Trial 2) were used. The fifth week was used for acclimation to the targeted environmental conditions. Weight gain was significantly higher in broilers exposed to AV of 1.5 and 2.0 m/s in both trials, which coincided with significantly higher feed intake and feed efficiency. Exposure to the low AV (0.5 and 1.0 m/s) resulted in weight gain, feed intake, and feed efficiency that were less than those of broilers exposed to high AV (2.5 and 3.0 m/s). In all treatments, broilers developed hyperthermia, but there were no significant differences in body temperature (Tb) among the birds. It can be concluded that AV has a significant effect on broiler performance; the optimal AV was found to be 1.5 to 2.0 m/s.

Air Movements↗

PubMed1999

Relationships among age, eggshell thickness and vitamin D metabolism and its expression in the laying hen.

Hens forming uncalcified shells synthesized less 1,25-hydroxycholecalciferol (1,25(OH)2D3) and less duodenal and eggshell gland (ESG) calbindin than normal laying hens. Hens forming thin shells had lower intestinal and ESG calbindin and its mRNA. Reducing ESG calcium (Ca2+) transport by the carbonic anhydrase inhibitor acetazolamide, but not by dietary Ca2+ restriction, reduced ESG calbindin and its mRNA. Two sub-populations of hens characterized by shell thickness (ST) maintained this characteristic throughout the whole production period. The differences between the two sub-populations increased with age. In old laying hens, the two sub-populations responded differently to dietary Ca2+ restriction and to exogenous 1,25(OH)2D3. Those forming a thin shell responded to 1,25(OH)2D3 by a significant improvement in ST. The results suggest that: (a) the mechanism responsible for Ca2+ transport to the egg shell consists of a vitamin D-dependent absorption of Ca2+ and a multi-factor-dependent transfer of Ca2+ to the shell; (b) both steps are, most likely, calbindin-mediated; however, the induction of calbindin gene expression in the ESG is predominantly calcium-dependent; and (c) the apparent defect in vitamin D metabolism or its expression in old hens is typical of, or even exclusive, to thin-shell-forming hens.

Acetazolamide↗

PubMed1998

Effects of age at onset of production, light regime and dietary calcium on performance, eggshell traits, duodenal calbindin and cholecalciferol metabolism.

1. Rate of production and shell thickness (ST) decreased, while body weight (BW), egg weight (EW) and percentage breakage increased progressively with age. Shell weight (SW) increased until 8 to 13 months of age and then decreased. 2. Early onset of production resulted in lower BW and EW at the onset of production, and lower pooled averages of BW, EW, SW and ST, as compared with late or medial onset of production. In 4 out of 5 trials, early onset did not result in the production of more eggs during the laying period. 3. Early onset of production is associated with physiological Ca deficiency as indicated by increases in kidney-1-hydroxylase and duodenal calbindin in early layers as compared with late layers. Early layers exhibited a more severe reduction in shell quality as the result of Ca deficiency as compared with late layers. 4. Feeding pullets with a prelaying diet containing 3.9% Ca did not affect unequivocally the performance or shell quality during the whole productive period, whether the birds started to lay early or late. The dietary treatment did not cause renal damage, as indicated by morphological examination and by plasma calcium and uric acid concentration.

Age Factors↗

PubMed1996

The role of gonadal hormones in gene expression of calbindin (Mr 28,000) in the laying hen.

Acute and chronic changes in calbindin (Mr 28,000) mRNA and calbindin concentrations were determined to assess the roles of steroid hormones in calbindin mRNA and calbindin synthesis in the eggshell gland (ESG). The results support an earlier suggestion that calbindin gene expression in the ESG is associated with Ca2+ flux through the ESG. The evidence includes wide oscillation of the mRNA during the diurnal egg cycle, in close temporal association with egg shell calcification. Progesterone (single im injection of 1 mg/kg body weight, BW) prolonged the period of egg formation and reduced the rate of Ca2+ transport and the concentration of calbindin mRNA in the ESG. Dexamethasone (single im injection of 5 mg/kg BW) prolonged the period of egg formation, increased shell Ca2+, and reduced calbindin mRNA in the ESG and intestine. Testosterone (single im injection of 2 mg/kg BW) did not affect calbindin mRNA synthesis in the ESG. The effects of estradiol on the synthesis of calbindin mRNA in the ESG of sexually immature or laying birds were minor, while it affected plasma Ca in the same birds. The antiestrogen Tamoxifen (60 mg/kg BW, given orally) reduced plasma Ca, but did not affect the synthesis of calbindin mRNA in the ESG. The antiprogesterone RU-38486 (20 mg/kg BW, orally) increased shell Ca2+ but had no effect on plasma Ca or the synthesis of calbindin mRNA. It appears that estrogens alone cannot account for the markedly elevated synthesis of calbindin mRNA in the ESG of the laying bird. The hypothesis that the regulatory mechanism for the synthesis of calbindin mRNA in the ESG may involve a stimulator(s), associated with the onset of production, and an oscillating depressor(s) is supported and both appear to be closely linked to the reproductive cycle. The specific in vivo effect of progesterone on calbindin mRNA in the ESG, together with its already known changes during the ovulatory cycle in birds, supports the idea that it is a depressor.

Animals↗

PubMed1992

Relationships between calbindin (Mr 28,000) and calcium transport by the eggshell gland.

1. Eggshell density (mg/cm2) and eggshell gland calbindin decreased in the aged hens. 2. Aged hens which laid eggs with a low shell weight and shell density had significantly lower intestinal and eggshell gland calbindin as compared with those which laid eggs with a high shell weight and shell density. 3. Significant correlations were found in aged hens between duodenal or eggshell calbindin and shell weight or shell density. 4. The results suggest that: (a) aged hens forming light shells absorbed calcium with a lower efficiency than those forming heavy shells or than young hens; (b) the decline in shell density in the aged hens is caused by a physiological calcium deficiency or by a defect in the hens' ability to alter calbindin synthesis in response to calcium needs; (c) in the aged hens, the deposition of calcium into the eggshell is dependent on, or at least associated with, eggshell gland calbindin.

Aging↗

PubMed1992

Regulation of calbindin mRNA and calbindin turnover in intestine and shell gland of the chicken.

A synthetic oligonucleotide was used as a probe for measurement of calbindin mRNA in the shell gland and intestine of chickens. The half time of calbindin mRNA in the duodenum and shell gland was estimated at 2 and 3.6 h and that of calbindin at 13.9 and 32.6 h, respectively. The formation rates of calbindin mRNA were 0.37 and 0.17 pmol.h-1.g-1 and the rate of calbindin formation was 0.099 and 0.031 microgram.pmol mRNA-1.h-1 in the duodenum and shell gland, respectively. In the shell gland, calbindin mRNA and calbindin appeared at the time of sexual maturation during calcification of the first egg shell. Calbindin mRNA fluctuated markedly during the daily egg cycle, in close temporal association with egg shell calcification. When Ca2+ deposition was eliminated by expulsion of the ovum, the rise in calbindin mRNA was prevented. An indirect suppression of Ca2+ deposition by administration of the carbonic anhydrase inhibitor acetazolamide also resulted in a decrease in calbindin mRNA. The results are consistent with a possible role of Ca2+ flux in the regulation of calbindin mRNA appearance in the shell gland of chickens.

Animals↗