PubMed Health⌕ Search

Biomedical subjects

E Wilmes

Publications and source records attributed to E Wilmes.

At least 55 records · Page 3Linked to original sources
PubMed1990

A comparison of class II antigenicity of human tracheal allografts stored in cialit and in merthiolate.

This report deals with some of the immunological aspects of the transplantation and preservation of human tracheal grafts. The immunological behavior of the transplanted graft depends largely on the interaction of the preservatives with the tissue proteins. Using monoclonal antibodies and the immunoperoxidase staining technique we have investigated the effect of Merthiolate, Cialit, formaldehyde/Merthiolate, and formaldehyde/Cialit preservation techniques on monomorphic determinants of class II transplantation antigens in human tracheal allografts. Unpreserved grafts were found to express class II antigens. These antigens were totally destroyed after 7 days in formaldehyde and 42 days in Cialit and Merthiolate. Preservation in Cialit and Merthiolate showed a gradual disintegration of the histological structures. In contrast, buffered formaldehyde did not appear to alter the histological structure of the tracheal graft. Irrespective of the mode of preservation, the cartilaginous tissue appeared to persist virtually unaffected. No essential differences were observed between the immunological staining of tracheal grafts preserved in Cialit and Merthiolate.

Cialit↗
PubMed1989

[Epstein-Barr virus infections. New pathogenic and clinical aspects].

The Epstein-Barr virus (EBV) is among the most widespread of human viruses. It causes several different diseases, such as acute infectious mononucleosis (IM), chronic active EBV-infection (cEBV), the x-linked lymphoproliferative syndrome (XLP), polyclonal and oligoclonal lymphomae in connection with immunologic disorders, as well as African Burkitt's lymphoma and nasopharyngeal carcinoma. Pathogenesis, clinical features and diagnosis are discussed. In this connection, special tests on the latency and multiplication of the Epstein-Barr virus are presented. --The Epstein-Barr virus persists in the parotid gland of healthy individuals and is secreted via saliva. It is transmitted by direct contact and responsible for infectious mononucleosis. For the first time we succeeded in proving the multiplication of the virus in the palatine tonsil during acute phase of infectious mononucleosis.

Burkitt Lymphoma↗
PubMed1989

[Viral origin of postnatally acquired inner ear damage].

Experimental and clinical data exist which point at a possible role of herpes virus infections in postnatally acquired inner ear disturbances. The presence of viral DNA in the inner ear and vestibular organ of experimentally infected mice and pigs suggests that not only primary infections but also the reactivation of latent herpes viruses can cause severe inner ear damage. Serological examinations are presented and therapeutic considerations discussed.

Acquired Immunodeficiency Syndrome↗
PubMed1987

[Attempts to produce human monoclonal antibodies to melanoma using the cervical lymph nodes].

Due to their specificity, constant properties and virtually unlimited supply monoclonal antibodies have given an important stimulus to almost every field of biomedical research within the last 10 years. The generation of mouse monoclonal antibodies includes immunisation of mice followed by fusion of mice spleen cells with a murine myeloma cell line. With this procedure hybridomas secreting monoclonal antibodies of a predefined specificity can be obtained. For three major reasons we worked on the establishment of human hybridomas secreting specific antibodies: human antibodies are less immunogenic when used for diagnostic or therapeutic purposes, only human monoclonal antibodies allow the analyses of the human B cell repertoire, there is evidence that human monoclonal antibodies recognise epitopes different from those seen by murine monoclonal antibodies. Therefore, we set out to generate human B cell hybridomas by cell fusion using the human lymphoblastoid B cell line Wi-L2-729 HF2 and lymphocytes from melanoma patients. The lymphocytes were isolated from tumour-draining cervical lymph nodes, stimulated with pokeweed mitogens plus the autologous tumour cells in an enriched tissue culture medium and fused in the presence of polyethylene glycol. Supernatants of hybridomas were screened in a single cell immunosorbent assay with either autologous melanoma cells or established melanoma cell lines fixed to the bottom of Terasaki plates or on cytospin preparations of these cells using the immunoperoxidase staining procedure. We could demonstrate that the tumour draining lymph nodes of these melanoma patients contained B lymphocytes capable of producing antibodies reacting with the tumour cells.(ABSTRACT TRUNCATED AT 250 WORDS)

Antibodies, Monoclonal↗
PubMed1987

[Transmissibility of HIV infections via allogeneic transplants].

HIV-infections can be transferred by blood and blood products, semen or organ transplants. For this reason, the WHO recommended a screening of all donors of blood, semen, tissues and organs. Those who belong to high-risk groups should be excluded from donations. If this recommendation should be also applied to cialit preserved auditory aussicles, this would probably mean the end of allogeneic transplantations of middle-ear surgery which are being performed daily. Therefore, we carried out experimental examinations with HIV-infected auditory aussicles in cialit. According to our results, it can be assumed that a virus-destruction takes place via a cialit-treatment. The same applies to solvent-dried fascia as to auditory aussicles which have been preserved in the same way. Consequently, there is no objection to further application of these allogeneic transplants in respect to HIV-infections.

Acquired Immunodeficiency Syndrome↗
PubMed1987

[Prevention of HIV infections (AIDS) in ENT practice and the clinic].

In comparison to sexual contact the transmitted droplet infection or the mucosa contact are of minor relevance for HIV, the causative agent of acquired immune deficiency (AIDS). These channels of infections should nevertheless not be disregarded. Due to his activity, connected with a very close contact with exudations of a wound, blood, saliva and mucosae, the ENT-specialist increased hazard of infection by the HIV virus. Since 80% of the infected persons are able to discharge viruses free from symptoms for years, the ENT specialist should be informed about the pattern of distribution of this infectious disease as well as about suitable methods of prevention. Recommendations to practice and clinic for the prevention of transmitting HIV infections are given.

Acquired Immunodeficiency Syndrome↗
PubMed1987

[A rare complication of septum surgery: internal carotid artery-cavernous sinus fistula].

Perforation, haematoma, a saddle nose, dysaesthesia, liquorrhoea and hyposmia are among the most common complications in septum plasties. A case history points out the danger of a fistula between the sinus cavernosus and the arteria carotis interna during this operation. A patient developed heavy nose bleeding during a septum plasty. A protrusio bulbi, a binocular deterioration in visual acuity, a pulsating tinnitus and a hyperaemic nose appeared postoperatively. By means of angiography of the arteria carotis interna the suspected diagnosis of an AV fistula was confirmed. The fistula was occuled in local anaesthesia by a radiopaque-filled micro-balloon. The possible direct approach using an instrument via the transseptal-transsphenoidal access and the indirect method of injury by breaking out one of the insufficiently mobilised spurs of the lamina perpendicularis and its extension, the dislocated septum interspenoidalis, are described and demonstrated by graphic representations.

Adult↗
PubMed1986

[Side effects of drugs on the salivary glands and mouth mucosa].

Changes to the salivary glands and the mucous membrane of the mouth can result from a substantial number of drugs. Since such changes are extremely heterogeneous, it is rarely possible to establish the medication responsible for the clinical-morphological findings. Furthermore, in many cases the symptoms resemble those associated with salivary gland or dermatological diseases, so that considerable diagnostic and differential-diagnostic problems can arise. A number of exemplary cases are presented and some differential-diagnostic recommendations are made for the practitioner.

Diagnosis, Differential↗
PubMed1984

Persistence of Epstein-Barr virus in the parotid gland.

Two independent techniques, in situ hybridization on frozen sections and reassociation kinetics, have been used to localize Epstein-Barr virus genomes in tissue samples from healthy human adults. Whereas specimens taken from the palatine tonsils were invariably negative, all samples from the parotid gland were positive when tested with either technique. This observation suggests that the parotid gland is, besides the peripheral lymphocytes, a site of lifelong persistence of Epstein-Barr virus and probably the site of low-level virus production which may be the source of virus found in the oropharynx.

Adult↗
PubMed1983

[Virus diseases in the ENT area].

Viral infections cause many disease in the mucous membranes, the upper respiratory and digestive tract, the salivary glands and the ear. Recent advances in the pathogenesis of viral diseases brought about an increasing interest of otolaryngologists. The general part of this paper presents the fundamental properties of viruses, their structure and replication, and the pathogenesis of the different forms of viral infections. The section of viral diagnosis describes laboratory methods and their limits as well as the clinical interpretation of viral tests. An overview of viral diagnosis describes laboratory methods and their limits as well as the clinical interpretation of viral test. An overview of prophylaxis and therapy of viral infections is given. The specialized section of the paper describes viral infections important for the otorhinolaryngologist. It includes infections of the aero-digestive tract, the ear and the salivary glands. Because of its special importance, Epstein-Barr virus and its associated diseases, and hypotheses about the oncogenesis of EBV-correlated tumours are presented in detail. The topic of the third part is of actual knowledge about interferon and its antiviral and antitumour effects. The findings of important clinical studies are discussed. The last chapter concerns with virushepatitis.

Animals↗
PubMed1983

[Tonsillar cancer and Epstein-Barr virus].

Epstein-Barr Virus is an important aetiological factor in the development of nasopharyngeal carcinoma (NPC). Serology suggested that besides NPC some other tumours located within the lymphoepithelial ring of the throat (Waldeyer's ring) might be associated with EBV. Nucleic-acid hybridizations were carried out to detect the presence of EBV-DNA in tumour biopsies derived from tonsillar carcinomas. We were able to demonstrate the presence of EBV-DNA in the tumour cells of two poorly differentiated carcinomas. We think that only a small fraction of tonsillar carcinomas is positive for EBV. The aetiologic relationship of EBV with tumours of Waldeyer's ring is discussed.

Animals↗