PubMed Health⌕ Search

Biomedical subjects

E Wilmes

Publications and source records attributed to E Wilmes.

At least 73 records · Page 4Linked to original sources
PubMed1981

[Epstein-Barr virus--a clinically relevant feature of nasopharyngeal carcinoma? (author's transl)].

Nasopharyngeal carcinoma (NPC) has been linked to Epstein-Barr Virus (EBV) by seroepidemiological evidence and by the regular proof of EBV-DNA in the epithelial tumor cells. We have been able to study the serological parameters of 62 NPC patients of the local ENT-Clinic. All patients were kaukasians in contrast to a previous study by Henle et al. Our results emphasize the remarkable predominance of EBV-IgA antibodies to viral capsid antigen (VCA) and early antigen (EA) in NPC patients and prove the value of the test for the initial diagnosis of the disease. Follow-up studies with subsequent serological tests strongly suggest that this test is related to the stage of the disease. We have also found NPC-typical serological EBV-IgA titers in 3 lymphoepithelial carcinomas of the tonsil and the soft palate. Similar titers have been found in two cases of poorly differentiated carcinomas of the base of the tongue. All these tumors arise in the lymphoepithelial tissue of Waldeyer's ring. We conclude that possibly some carcinomas of Waldeyer's ring are similarly related to EBV as nasopharyngeal carcinomas are.

Aged↗
PubMed1981

[Evidence for the persistence of Epstein-Barr-virus in the parotid gland (author's transl)].

EBV is associated with B-lymphocytes. It is, however, not clear whether spontaneous activation of EBV genomes in carrier lymphocytes, which are present in the lymphocyte rich area of the oropharynx, is responsible for lifelong persistence of antibody-titers directed against EBV related antigens and the shedding of virus into the oropharynx. Alternatively EBV could reside in specific sites of the body and be produced there resembling somehow the situation of Marek's disease virus of chicken. That virus is found in T-lymphocytes and produced and spread from the epithelium of featherfollicles. In situ hybridisations with frozen sections of tonsils and parotid glands reveiled that EBV genomes are present in the tissue of the parotid gland of healthy seropositive persons. The data from in situ hybridisations could be confirmed using stringent reassociationkinetics. It is suggested that spontaneous activation of the lymphocytes is a very rare event and neither the source of virus in the saliva nor the reason for the lifelong persistence of antibodies to EBV related antigens.

Antibodies, Viral↗
PubMed1979

[Significance of virus-specific antibodies to EBV-antigens in patients with nasopharyngeal carcinoma (author's transl)].

Sera collected from patients with nasopharyngeal carcinoma, patients with other head and neck neoplasms, patients with infectious mononucleosis (IM), and healthy donors were titrated for antibodies (IgM, IgG, IgA) against Epstein-Barr-Virus (EBV) specific antigens (VCA, EA, EBNA) by indirect and by antikomplementary immunfluorescence. NPC-patients develop significantly high anti-EBV titers of IgG and IgA. In contrast patients with neoplasms (mainly carcinomas) arising in sites of the head and neck other than the nasopharynx revealed a lower incidence of high titers. Our results emphasize the remarkable predominance of IgA-antibodies to VCA and EA in NPC-patients and shows that Europeans to not differ in that respect from Asian patients. The significance and implications of these findings are discussed.

Antibodies, Viral↗
PubMed1979

[Studies for proteolysis in malignant tumours (author's transl)].

It's well known that increased proteolytic activity in plasma as well as in interstitial fluids is often associated with malignant tumours. There are numerous reports that cell cultures of malignant origin contain plasminogen activators responsible for an increased proteolysis. Our studies showed that patients with a carcinoma excrete in their urine a significant higher amount of an acid-stable inhibitor than healthy patients. This urine inhibitor is a split product (MW: 30,000) of the Inter-alpha-trypsin inhibitor (MW: 180,000). We conclude that the increased amount of acid-stable split products is the result of increased proteolysis in patients with malignant tumours. In contrast to other authors we do not think that the activation of plasmin by plasminogen is responsible for the proteolytic cleavage of ITI. Otherwise proteolysis by Cathepsin G and granulocytic Elastase would be reasonable. So enhanced proteolysis seems to be a sign of general enzymatic change associated with transformation.

Carcinoma, Squamous Cell↗
PubMedDate not supplied

Detection of class II antigens on human nasal cartilage.

The presence and distribution of class II antigens on human nasal cartilage was investigated using monoclonal antibodies directed against HLA-DR, HLA-DP, and HLA-DQ molecules. Tissue sections and chondrocytes in suspension were prepared for immunoperoxidase staining and flow cytometric analysis. Class II antigens were detected primarily on the perichondrium. In contrast, chondrocytes, either isolated or in tissue sections, were completely negative, suggesting that cells of the perichondrium, may, by themselves, be capable of initiating a rejection process.

Antibodies, Monoclonal↗
PubMedDate not supplied

Recommendations for therapy of head and neck cutaneous melanoma.

PURPOSE: The essential element in the treatment of a primary cutaneous malignant melanoma is an adequate definitive excision. The breadth and depth of such excisions and the appropriateness of a prophylactic neck dissection, however, remain a source of controversy. METHOD: A review of our experiences with 500 patients with head and neck malignant melanoma treated in our clinic between 1967 and 1987 is presented. RESULTS AND CONCLUSIONS: The thickness of the lesion, the factor that correlated most closely with potential for metastatic development, can be used as a guide for determining the extent of excision and the appropriateness of elective node dissection. Wide excision of invasive lesions varies in their margins from 1 cm for lesions that measure less than .75 mm in thickness to 3 cm if the melanomas measure greater than .75 mm or show ulcerations or have a scalp localization. Prophylactic neck dissection is necessary for lesions between .75 and 1.5 mm in thickness, whereas in tumors with a depth of invasion greater than 1.5 mm the outcome of the disease is not improved by prophylactic neck dissection.

Head and Neck Neoplasms↗
PubMedDate not supplied

Human immunodeficiency virus cannot productively infect freshly cultured human cartilage cells.

The use of cartilage allografts is being discussed because of the possible transmission of the human immunodeficiency virus (HIV). To further delineate the possibility for an HIV infection of cartilage cells the susceptibility and permissivity of normal human chondrocytes to HIV-1 was assessed in culture. Isolated cartilage cells were incubated during 30 days with the HIV, testing the production of viral p24 antigens and the formation of particle-bound reverse transcriptase (RT) in the supernatant. The H9 cell line was used as positive control. The production of p24 antigen did not occur on chondrocytes during the whole culture time. Furthermore, no RT activity was detected throughout the entire experimental period. Our results indicate clearly that HIV was not able to infect cartilage cells under these in vitro conditions, and, conclusively, HIV infection of cartilage in vivo is improbable.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

Synthesis of human cartilage using organotypic cell culture.

The limited supply of fresh autologous cartilage tissue for use in reconstructive surgery necessitates the use of vital banked allografts. A feasible in vitro production of cartilage tissue composed of living cells requires the use of modern tissue culture techniques retaining the phenotypic characteristics of chondrocytes. With this purpose in mind, human chondrocytes were isolated and cultured using different culture procedures: monolayer, suspension and agar gel. The differentiation state of chondrocytes as well as proteoglycan and collagen syntheses were assessed by histochemical and immunohistochemical methods. Whereas chondrocytes in monolayer displayed an unstable phenotype and tended to dedifferentiate, in three-dimensional culture the chondrocytes remained morphologically, phenotypically and functionally differentiated. Furthermore, an accumulation of matrix products pericellularly was observed in the agar gel. The results suggest that three-dimensional cultures in agar gel may allow the in vitro production of bioartificial cartilage for transplantation.

Agar↗
PubMedDate not supplied

Tracheal transplantation: demonstration of HLA class II subregion gene products on human trachea.

The present morphological study was designed to investigate the expression of HLA class II subregion gene products on human trachea. Frozen sections from the tracheas of 20 cadavers not suffering from ear, nose or throat diseases were studied immunohistochemically using monoclonal antibodies which recognize monomorphic determinants of HLA-DR, -DP and -DQ molecules. Positive reactions could be detected in the airway epithelium and mixed glands. HLA-DR and -DP showed a stronger presence than HLA-DQ. Our results indicate that tracheal mucosa may be the major antigenic structure of the trachea and therefore responsible for the immunogenic action of allogenic tracheal transplants.

HLA-DP Antigens↗
PubMedDate not supplied

[Oral hairy leukoplakia in human immunodeficiency virus positive patients: clinical and etiopathogenic aspects].

Clinical and etiopathogenetic features of oral hairy leukoplakia in 5 patients positive for Human Immunodeficiency Virus were studied. Oral hairy leukoplakia were located on the lateral borders of the tongue and showed a corrugated/hairy aspect in all the cases. Histological examination showed hyperparakeratosis, acanthosis, hair like projection (n = 4) koilocyte-like-cells and moderate subepidermal inflammation. Immunohistochemistry revealed positive results for Epstein-Barr virus indicating an active replication of this virus within the epithelial cells of the stratum granulosum and upper stratum spinosum.

Adult↗
PubMedDate not supplied

[The presence of the Epstein-Barr virus in Waldeyer's lymphatic ring during the acute phase of infectious mononucleosis].

The aim of our research was to detect the existence of virus Epstein-Barr genome in the palatine- and the nasopharyngeal tonsil obtained from six patients during the acute phase of the infectious mononucleosis. The presence of EBV-DNA was demonstrated with the help of nucleic acid hybridization techniques (Southern-Blot and in situ hybridization) in the two components of the lymphoepithelial ring of the throat. The pathogenesis and the therapeutic implications of these findings are discussed.

Acute Disease↗
PubMedDate not supplied

[Malignant otorhinolaryngological lymphomas associated with human immunodeficiency virus infection].

Six instances of lymphoma occurring in homosexual male patients among 140 HIV-positive subjects attended at our Department of Otolaryngology were evaluated for clinical features, histopathologic features and Epstein-Barr virus (EBV) DNA. The histology of the patients was consistent with a Hodgkin's lymphoma, centroblastic lymphoma and four lymphoblastic lymphoma. High malignancy and nodal localization were characteristic of four non-Hodgkin's lymphoma, which carries a poor prognosis. The DNA in situ hybridization studies demonstrated the presence of EBV DNA sequences in the four lymphoblastic lymphoma.

Adult↗
PubMedDate not supplied

[Otorhinolaryngologic manifestations in patients infected by the acquired immunodeficiency virus].

Patients with human immunodeficiency virus (HIV) infection often present signs and symptoms referable to the head and neck. We reviewed the clinical histories of 110 HIV-positive patients who presented head and neck manifestations. 48 (43.6%) had head and neck signs and/or symptoms as initial manifestation of the syndrome. 40 (36.3%) had oral candidiasis, 18 (16.36%) Kaposi's sarcoma, 11 (10%) herpes, 5 (4.5%) hairy leucoplakia and 4 (3.6%) lymphoma.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

[Presence and distribution of HLA-DR, HLA-DP and HLA-DQ transplant antigens in the human tympanum].

The present morphological study was designed to investigate the expression of HLA class II subregion gene products on human tympanic membrane. Frozen sections from tympanic membrane of 10 cadavers were studied immunohistochemically using monoclonal antibodies. Positive reactions could be detected in the epidermal layer and the subepidermal connective tissue layer of the lamina propia. The implications of our findings on the clinical field of tympanic membrane allografting are discussed.

Histocompatibility Antigens Class II↗