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Biomedical subjects

E Wulf

Publications and source records attributed to E Wulf.

At least 19 recordsLinked to original sources
PubMed1989

[Chemoluminescence study of the influence of phagocytosis of peripheral blood leukocytes using a perfluorocarbon-containing blood substitute].

In an animal experimental model (rats) the influence of which model substances for blood substitution of GDR origin may have on the phagocytosis behaviour of leukocytes of the peripheral blood was investigated by means of luminol-amplified chemoluminescence. After applying the model substance in an amount corresponding to 10% of the circulating blood volume a reversible increase of luminol-amplified chemoluminescence could be observed. The values referred to the portion of neutrophilic granulocytes, however, showed no significant differences compared to the control groups. The opsonizing capacity of the serum towards cymosan revealed a temporary deficit after applying blood substitution substances of GDR origin. The conclusion is drawn that the functions of leukocytes of the peripheral blood recorded by the applied method are not depressed by the model substances used.

Animals↗
PubMed1989

Changes of immunological parameters in immunocompromised patients under selective decontamination of the digestive tract. Third Part: Behaviour of some plasma proteins.

We studied the importance of changes in plasma protein concentrations in patients with hematologic systemic diseases treated by selective decontamination of the digestive tract. The concentrations of albumin, acute-phase proteins, immunoglobulins and fibronectin were determined by laser nephelometry in 125 serum samples of eight patients. Results obtained allow the following conclusions: 1. There was a correlation between the clinical condition and concentrations of acid alpha-1-glycoprotein, IgG, CRP and fibronectin. These concentrations might be important for assessment of the course of the disease, effectiveness of therapy and the detection of pathogenetic relations. 2. The acid alpha-1-glycoprotein is of particular importance since relations exist between changes in its concentration and therapeutic results as well as the prognosis of the disease.

Acute-Phase Proteins↗
PubMed1989

[The laser nephelometric determination of C-reactive protein].

A method for the laser nephelometric determination of C-reactive protein (CRP) is described, using easily available reagents. The upper limits of the reference ranges were determined for newborns and adults. They are 12 mg/l and 27 mg/l, respectively. A comparison with the radial immunodiffusion, which is a widespread method in the clinical laboratory, showed significantly different (higher) values for the laser nephelometry as well as a higher reliability in detecting only slightly raised concentrations. The capillary test proved to be not suitable for the quantification of CRP, but is sufficiently reliable for the detection of sera with pathologically elevated CRP-concentrations.

Adult↗
PubMed1989

Changes of immunological parameters in immunocompromised patients under selective decontamination of the digestive tract. Second Part: Investigations on the influence of drugs on zymosan-induced and luminol-enhanced chemiluminescence of peripheral leucocytes.

The influence of drugs used for selective decontamination, given in therapeutically effective concentrations, was examined in healthy controls and immunocompromised patients with hematologic systemic diseases by measuring the zymosan-induced and luminol-enhanced chemiluminescence of peripheral leucocytes. Drug-induced repression of phagocytic activity could usually be found both in healthy controls and in patients with hematologic systemic diseases. The combination of trimethoprim and sulfamerazine had a pronounced repressive effect. Such indications should be taken as a basis for further investigations in order to avoid additional iatrogenic restriction of defence. If possible, drugs with effects leading to repression of phagocytosis should not be used for selective decontamination.

Anti-Infective Agents↗
PubMed1986

Changes of plasma fibronectin concentration and phagocytic activity in immunosuppressed patients during selective decontamination.

Plasma fibronectin and phagocytic activity play important roles in combating infections. The question is discussed, whether both defense systems are also of importance in immunosuppressed patients. Further, the behaviour of plasma fibronectin determined by laser nephelometry, and phagocytic activity determined by chemiluminescence is demonstrated in patients with leukaemia under the conditions of selective decontamination of the intestinal tract. The following results are shown: Plasma fibronectin concentration decreases 10 to 14 days before onset of the first clinical signs of an infection. Plasma fibronectin level changes appear earlier than that of C-reactive protein (Crp). Therefore, it is suitable as a parameter for assessment of the course of an infection. Decreased plasma fibronectin levels occurring over longer periods have to be regarded as unfavourable prognostic criterion. The phagocytic activity of immunosuppressed patients selectively decontaminated is significantly below that of healthy adults. A clear assignment of phagocytic activity to the clinical picture, the number of granulocytes and plasma fibronectin level is not possible at present. Additional studies are necessary. Both plasma fibronectin level and phagocytic activity do not appear to be influenced by selective decontamination of the intestinal tract.

Anti-Bacterial Agents↗
PubMed1985

[Early diagnosis of primary chronic glomerulonephritis in the latency stage].

In 74 of 195 patients with isolated proteinuria and/or haematuria investigated by means of renal biopsy laser-nephelometric determinations of the concentrations of albumin, alpha2-macroglobulin, transferrin, IgG, IgA and IgM in the urine were performed. Their result was that patients with chronic interstitial nephritis or chronic glomerulonephritis by increased concentration of albumin, transferrin, IgG and IgA statistically ascertained differ from patients with normal renal tissue or slight glomerular abnormalities.

Chronic Disease↗
PubMed1984

[Methodologic references for clinical function diagnosis of glomerular permeability selectivity by molecular wight related protein analysis and additional para-aminohippuric acid load].

Glomerular perm selectivity can be approximately estimated from the apparent glomerular protein clearance in terms of molecular weight since the relative protein clearance is approximately identical with the corresponding screen coefficient relation. Systematic relativization of individual protein clearances by reference to albumin (or transferrin) clearance requires the application of special calculations to find out the regression coefficients relevant to functional diagnosis. A PAH load influences tubular protein reabsorption so that the clearance of higher-molecular proteins (IgA alpha 2-macroglobuli, IgM) rises. Taking the changes in protein clearance under renotubular PAH load into account allows a more precise estimation of glomerular permeability to be made, in comparison with protein analyses not using PAH load.

Aminohippuric Acids↗
PubMed1981

Ether derivatives of alpha-amanitin. Introduction of spacer moieties, lipophilic residues, and radioactive labels.

Etherification of alpha-amanitin with tritiated methyl iodide yielded a radioactively labeled amatoxin of high specific activity (similar to or approximately 4 Ci/mmol) which, in its inhibition capacity for RNA polymerase II, was very similar to alpha-amanitin. The labeled toxin was used successfully in binding assays with RNA polymerases II and in radioimmunological determinations of amatoxins. If long-chained alkyl bromides were reacted with alpha-amanitin, lipophilic ether derivatives were obtained with a facilitated penetration capacity into cells. As a consequence of the improved permeability, two derivatives, O-hexyl- and O-decyl-alpha-amanitin, were more toxic in vivo than alpha-amanitin, although their affinity to RNA polymerases II was much reduce. By reaction of N-tert-butyloxy-carbonyl-N'-(6-bromocaproyl)ethylenediamine with alpha-amanitin, a ten-atom spacer with a terminal amino group could be introduced into the toxin, which allowed the attachment of alpha-amanitin to proteins, solid-phase supports, or reporter groups. For example, by reaction with fluoresceinyl isothiocyanate, a fluorescent amatoxin was prepared for visualizing amatoxin-binding structures in cells. After succinylation of the spacer moiety, alpha-amanitin could be attached to proteins, e.g., fetuin, yielding a derivative with good antigenic properties. When an alpha-amanitin derivative was coupled to Sepharose 6B, an adsorbent for affinity chromatography was obtained suitable for a one-step purification of amatoxin-binding immunoglobulins from the sera of immunized rabbits.

Amanitins↗
PubMed1979

Fluorescent phallotoxin, a tool for the visualization of cellular actin.

A fluorescent derivative of phalloidin has been synthesized possessing high affinity to filamentous actin. This compound was used for visualization of actin-containing structures in eukaryotic nonmuscle cells. Due to its low molecular weight (1250), fixation for formaldehyde was sufficient to render the membrane permeable for the labeled peptide. Bundles of microfilaments are the predominant pattern in the flat rat kangaroo PtK1 cells, whereas a net of concentric fibers characterizes the more spherical bovine kidney MDBK cells. Specificity of staining was confirmed by competition experiments with unlabeled phalloidin.

Actins↗