Biomedical subjects
E de Maeyer
Publications and source records attributed to E de Maeyer.
Production by mixed lymphocyte cultures of a type II interferon able to protect macrophages against virus infection.
In supernatants of mixed mouse spleen cell cultures established for 4 days, a species-specific inhibitor of virus replication with a broad antiviral spectrum was found. The inhibitor was destroyed by trypsin, was nondialyzable and acid labile, and was not neutralized by antibody to mouse L cell interferon. This indicates that in mixed lymphocyte cultures a type II interferon is made that has no immunological relationship with "fibroblast" interferon. This leukocyte product was shown to protect mouse hepatitis viruses. It is suggested that lymphocyte interferon may collaborate with macrophages in host defense against viruses, as a mediator of cellular immunity.
[The chromophore of blue dextran: a powerful ligand for the purification of interferon by affinity chromatography].
The chromophore of blue dextran can be used as ligand for affinity chromatography of interferon. One passage of a crude interferon preparation on a blue-dextran-sepharose column eliminates over 90% of the contaminating proteins. Desorption with an aqueous buffer of high molarity yields interferon preparations with specific activities of up to 4.5 X 10(6) units per milligram of protein and the recovery is 100%.
A locus affecting circulating interferon levels induced by mouse mammary tumour virus.
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[Prolongation, by interferon, of the survival of skin grafts in the mouse].
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Purification of mouse interferon by affinity chromatography on a solid-phase immunoadsorbent.
A solid-phase immunoadsorbent capable of binding mouse interferon has been prepared. Starting from crude tissue-culture material, interferon could be purified 1990 times in a single step of affinity chromatography. Overall recovery ranged from 55 to 103% with tissue culture and mouse-brain interferon; however, only 5% was obtained with Sendai virus-induced interferon from mouse serum.
Interferon messenger RNA: translation in heterologous cells.
A viral inhibitor with the characteristics of mouse interferon is produced by avian and simian cells preincubated with RNA extracted from interferon-producing mouse cells. Similarly, RNA extracted from interferon-producing monkey cells induces a monkey interferon-like substance in avian cells and also in a line of simian cells, VERO, which normally lacks the capacity to produce its own interferon. In both cases, the RNA effect is inhibited by treatment of the receptor cells by cycloheximide, but not by actinomycin D. We conclude that interferon messenger RNA has been translated in the receptor cells. Thus, the production of interferon in heterologous cells can be used as a sensitive assay of interferon messenger RNA.
Effect of antilymphocytic serum on circulating interferon in mice as a function of the inducer.
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[Interferon production at the level of the macrophage].
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Interferon synthesis in x-irradiated animals. IV. Donor-type serum interferons in rat-to-mouse radiation chimeras injected with Newcastle disease virus.
C(3)H/He mice were exposed to total-body X-irradiation of 1000 roentgens and received thereafter 10(7) xenogeneic Wistar rat marrow cells intravenously. In these rat-to-mouse chimeras, serum interferon-producing capacity upon injection of Newcastle disease virus was examined four weeks after grafting. Normal levels of circulating interferon were produced. The interferon, however, had the species specificity of rat interferon, being 20 times more active in rat embryo fibroblasts than in mouse embryo fibroblasts. Moreover, it behaved like rat interferon when filtered on Sephadex G-100 dextran gel, displaying one peak of activity in the 90,000 molecular-weight range and two incompletely separated peaks of 32,000 and 24,000, respectively, in the 30,000 molecular-weight region. The fact that the interferon is of the donor type in rat-to-mouse chimeras permits us to conclude that circulating interferon induced by New-castle disease virus is made in bone-marrow-derived cells.
Inhibition of interferon synthesis by triethylenemelamine and 4-nitroquinoline-N-oxide.
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Interferon synthesis in x-irradiated animals. II. Restoration by bone-marrow transplantation of circulating-interferon production in lethally-irradiated mice.
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Interferon synthesis in x-irradiated animals. I. Depression of circulating interferon in C3H mice after whole-body irradiation.
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