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Purification of mouse interferon by affinity chromatography on a solid-phase immunoadsorbent.

J D Sipe·J de Maeyer-Guignard·B Fauconnier·E de Maeyer
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Abstract

A solid-phase immunoadsorbent capable of binding mouse interferon has been prepared. Starting from crude tissue-culture material, interferon could be purified 1990 times in a single step of affinity chromatography. Overall recovery ranged from 55 to 103% with tissue culture and mouse-brain interferon; however, only 5% was obtained with Sendai virus-induced interferon from mouse serum.

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AnimalsCells, CulturedChromatographyChromatography, AffinityImmune SeraImmunoglobulinsInterferonsMethodsMiceMice, Inbred C57BLNewcastle disease virusParainfluenza Virus 1, HumanPolysaccharidesSheepJournal Article

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J D Sipe, J de Maeyer-Guignard, B Fauconnier, E de Maeyer. 1973. Purification of mouse interferon by affinity chromatography on a solid-phase immunoadsorbent.. https://doi.org/10.1073/pnas.70.4.1037

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