A standard protocol for obtaining fish chromosomes under post-mortem conditions.
In order to improve cytogenetical analyses on fish, especially focusing on delicate and rare species, we have adopted a new in vitro methodology using dead animals. The results obtained from 24 neotropical freshwater and marine fish species demonstrate that chromosomes can be obtained under post-mortem conditions. Significantly, the samples analyzed provided reliable cytogenetical data in nearly all cases. Other advantages of this new methodology are also discussed.
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