A standard protocol for obtaining fish chromosomes under post-mortem conditions.

Maria Rita de Cáscia Barreto Netto; Erica Pauls; Paulo Roberto A de Mello Affonso

doi:10.1016/j.micron.2006.07.019

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A standard protocol for obtaining fish chromosomes under post-mortem conditions.

Maria Rita de Cáscia Barreto Netto·Erica Pauls·Paulo Roberto A de Mello Affonso

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Abstract

In order to improve cytogenetical analyses on fish, especially focusing on delicate and rare species, we have adopted a new in vitro methodology using dead animals. The results obtained from 24 neotropical freshwater and marine fish species demonstrate that chromosomes can be obtained under post-mortem conditions. Significantly, the samples analyzed provided reliable cytogenetical data in nearly all cases. Other advantages of this new methodology are also discussed.

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Animals Chromosomes Cytogenetic Analysis Fishes Microscopy Journal Article Research Support, Non-U.S. Gov't

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Maria Rita de Cáscia Barreto Netto, Erica Pauls, Paulo Roberto A de Mello Affonso. 2006-09-05. A standard protocol for obtaining fish chromosomes under post-mortem conditions.. https://doi.org/10.1016/j.micron.2006.07.019

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