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F Algaba

Publications and source records attributed to F Algaba.

100 records · Page 6Linked to original sources
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Differential diagnosis and evaluation of the clinical course of transurethrally resected T1G3 urothelial carcinoma of the bladder by DNA image cytometry.

The value of DNA image cytometry in the differential diagnosis of 106 T1G3 urothelial carcinomas of the bladder and the long-term prognosis (recurrence-free interval, survival) of the patients was tested in comparison with Ta/T1G1 (n=30) and Ta/T1G2 carcinoma (n=54). Monolayer smears were prepared from three 50-microm-thick sections by a cell separation technique and were stained according to Feulgen. The DNA content of 250 epithelial cells, chosen at random, was determined using a TV-image analysis system CM-1 (Hund, Wetzlar, Germany). The DNA content of 30 lymphocytes served as an internal standard for the normal diploid value in every individual case. Different DNA cytometric parameters and the mean nuclear area were calculated. In comparison with G1- and G2-cases, the mean values of all DNA cytometric variables were markedly increased in the group of T1G3 cases, most obviously for the 5cEE, the mean ploidy and the ploidy imbalance (0.0006 > or = p > or = 0.0001). However, a remarkable overlay of the data distribution had to be considered. An aneuploid DNA stemline ploidy was highly characteristic for T1G3 urothelial carcinoma (sensitivity: 92%), but not sufficiently specific (57%). However, if increased values for the mean ploidy, the 2cDI, the 5cEE or the 9cEE (specificity: 86%-89%) were present additionally, the diagnosis of a T1G3 urothelial carcinoma could be made cytometrically. Follow-up data for survival (recurrence) analysis was available for 90 (82) patients of the T1G3 group. Using the median value as threshold, significant differences in survival were found for the mean ploidy only (p=0.0353). The length of the recurrence-free interval was significantly different for the entropy (p=0.0205), the 2cDI (p=0.0309) and the mean ploidy (p=0.0442). In conclusion, DNA single cell cytometry represents a highly relevant tool in the objective identification of T1G3 urothelial carcinoma of the bladder, with a sufficient sensitivity and specificity. Further, this method enables prediction of tumor recurrence if suitable variables are chosen. The long-term survival of patients with T1G3 urothelial carcinoma can be estimated by DNA cytometry only in a limited manner, possibly due to the fact that the causes of death in the mostly elderly patients will be independent from the limited tumor disease.

Aged↗
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Molecular staging of bladder cancer with RT-PCR assay for CK20 in peripheral blood, bone marrow and lymph nodes: comparison with standard histological staging.

BACKGROUND: The aim of this study was to analyze whether the CK20 reverse transcriptase polymerase chain reaction (RT-PCR) is suitable for detecting circulating tumor cells and residual tumor cells in lymph nodes, in patients with muscle invasive transitional cell carcinoma (TCC) of the bladder, and to compare these results with standard histological staging. PATIENTS AND METHODS: The nested RT-PCR assay was used to analyze the CK20 transcript in the peripheral blood, bone marrow, lymph nodes, the tumor and normal biopsies of bladder from 57 patients with invasive TCC of the bladder, who underwent radical cystectomy, and from 9 patients with noninvasive TCC. RESULTS: Lymph node pathological status was positive in 24 out of the 57 patients studied and all of them except I showed expression of CK20, with a correlation between histological technique and RT-PCR of 95.8%. A statistically significant correlation of lymph node CK20 RT-PCR with the standard risk factor of pathological stage (p = 0.04) was observed Blood and bone marrow CK20 RT-PCR showed no correlation with pathological stage. CONCLUSION: Lymph node CK 20 RT-PCR correlates with pathological stage in bladder cancer. The CK20 RT-PCR assay appears to be a highly sensitive and specific method for detecting circulating tumor cells and residual disease in lymph nodes in patients with invasive bladder cancer. Further evaluation of the significance of CK20 as a molecular marker for staging and follow-up in these patients is necessary.

Adult↗
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[Papillo-polypoid cystitis. Focal cystitis with pseudoneoplastic aspect].

Report on 12 patients with vesical papillary changes of the mucosa, endoscopically assessed as tumours, but where the pathological study showed to be no epithelial neoplastic changes, since exophitical growth was a consequence of the submucosal edema (91.6%) with a moderate inflammatory reaction. Twenty-five percent cases exhibited no causative factors and 75% were correlated to urethral catheter (average 2.3 months). Due to major the and microscopic similitude between low degree transitional cell carcinoma and the localized inflammatory changes (papillary cystitis) the criteria to establish a correct diagnosis are discussed.

Adult↗
PubMedDate not supplied

[Endoscopic biopsy in the staging of infiltrating tumor of the bladder].

From July, 1990 to June, 1991, a prospective study was conducted on 65 patients with infiltrating bladder tumor to determine the possibility of distinguishing T2 from T3 tumors endoscopically. Sixty-six tumors were resected, including all of the bladder wall, and samples of perivesical fat were obtained from the tumor bed. The pathologist found infiltration of the bladder muscle wall in 50 cases (75.6%) and classified 12 tumors as T2 and 38 as T3. Fifty-five per cent of the T3a tumors were reclassified as T3b on the basis of the indirect signs of the pelvic scan and bimanual palpation performed under anesthesia. The clinical staging correlated with the pathological findings in 52.6% of the 19 cases of pT3b that underwent radical cystoprostatectomy; 31.7% of the pT3b tumors were clinically understaged, mainly due to prostatic infiltration. We can conclude from our findings that the differential diagnosis between pT2 and pT3 tumors is possible by endoscopic resection for most of the infiltrating bladder tumors; however, pT3a tumors cannot be distinguished from pT3b tumors with sufficient reliability.

Biopsy↗