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Biomedical subjects

F Ambrogi

Publications and source records attributed to F Ambrogi.

At least 55 records · Page 3Linked to original sources
PubMed1990

Phytohaemagglutinin-induced neutrophil aggregation in patients affected by chronic myeloproliferative diseases.

The aim of this work was to evaluate the phytohaemagglutinin-induced aggregation of circulating neutrophils isolated from 25 patients affected by chronic myeloproliferative syndromes (polycythaemia vera, chronic myeloid leukaemia in chronic phase, and essential thrombocythaemia). The results showed a lesser aggregating capacity in chronic myeloid leukaemia (CML) and an opposite behaviour in polycythaemia vera and essential thrombocythaemia. Patients with polycythaemia vera whose neutrophils showed a greater aggregating capacity were shown to have had various vascular complications.

Adult↗
PubMed1989

[Role of vitamin D3 in hematology].

It has been recently shown that 1,25(OH)2D3 induces differentiation and inhibits the proliferation of normal and leukemic cells in vitro. A review of the literature and the preliminary clinical applications of this vitamin in hematology are reported.

Blood Cells↗
PubMed1989

Serum cholesterol and triglycerides in hematological malignancies.

Serum levels of total cholesterol and triglycerides were studied in 202 patients affected by various hematological malignancies at the time of diagnosis. A hypocholesterolemia was found in 44% of patients affected by lymphoproliferative diseases and acute lymphoblastic leukemia, with an evident correlation with the clinical stage (5.7% of patients in nonadvanced stages, 67.8% in advanced stages). In acute and chronic myeloproliferative diseases, the overall incidence of hypocholesterolemia was 71%. In particular, a greater incidence of low cholesterol values was found in chronic myeloid leukemia and in idiopathic myelofibrosis than in polycythemia vera. No significant correlation was found in this group of diseases between the values of cholesterol and the main hematological parameters studied (WBC, number of circulating blasts, degree of splenomegaly, levels of hemoglobin, hematocrit). The incidence of significant alterations of triglycerides appeared negligible. It is thus possible to affirm that hypocholesterolemia constitutes an interesting biological aspect in hematological malignancies, and that total cholesterol could represent a parameter, even though secondary, in the follow-up of hematological neoplastic pathologies.

Adolescent↗
PubMed1989

Modulating effects by Trichinella spiralis sensu stricto excretory/secretory antigens of human neutrophil functions.

Many parasites are able to modify the host response through different mechanisms, for example host leukocyte function modification. Since granulocytes represent the most important effector cells against Trichinella spiralis, we evaluated the effects of supernatants of T. spiralis sensu stricto muscle larvae cultured for at least 18 hours (T. Sup.) on some human neutrophil functions, i.e. chemotaxis, phagocytosis and Nitroblue tetrazolium dye reduction. The cells from blood donors were tested in the presence of T. Sup. at different concentrations (1, 5, 10, 20%). The results show no effect of T. Sup. on neutrophil phagocytosis. On the contrary, random migration of cells is increased at concentrations of 10-20%, while stimulated migration is significantly decreased at concentrations of 5-10% but not at 20%. We found a significant decrease of Nitroblue tetrazolium reduction at 5-10% concentrations of T. Sup. It is concluded that T. spiralis culture supernatants contain some factors capable of modifying human neutrophil chemotaxis, oxidative metabolism, but not phagocytosis.

Animals↗
PubMed1987

Chronic lymphocytic leukemia (B-cell) in the course of polycythemia vera. Description of a case with an unusual chromosomic anomaly.

The present paper describes the case of a patient who developed a B-cell chronic lymphocytic leukemia (B-CLL) 15 months after the diagnosis of polycythemia vera, which had been treated only with phlebotomies. In spite of lymphocytosis and the clinical signs and symptoms of leukemia, the patient exhibited at the same time presumptive elements of polycythemia (high LAP index levels, a high number of neutrophils). Cytogenetic investigations, carried out after the appearance of B-CLL, revealed the presence of an unusual abnormality (18 p+) both in bone marrow not stimulated by mitogens and in PWM-stimulated circulating lymphocytes. This case, which is the ninth of its kind described in the literature, offers some interesting observations about the association between myeloproliferative and lymphoproliferative syndromes.

Aged↗
PubMed1987

Experimental studies on diacerhein: effects on the phagocytosis of neutrophil cells from subcutaneous carrageenan-induced exudate.

Diacerhein (DAR), a new drug which is particularly suitable for the treatment of osteoarthritis, was studied for its interference with the phagocytic capacity of cells coming from exudates of subcutaneous carrageenan oedema and from the peripheral blood of Sprague-Dawley rats. DAR was found to inhibit phagocytosis in both types of cells examined. This finding indicates that DAR may exert its action by means of a direct effect on the cells involved in the inflammatory process.

Animals↗
PubMed1987

Studies in vitro on the effects of rhein on the chemotaxis of human leukocytes.

Rhein (R: 1,8-dihydroxy-3-carboxyanthraquinone) is the active metabolite of the drug diacetylrhein (DAR), an anthraquinone molecule which has recently been proposed for the long-term treatment of osteoarthrosis. Its action mechanism in rheumatic pathology has not been fully explained. It is known that DAR, while not inhibiting the formation of prostaglandins, inhibits certain proteolytic enzymes, and acts on phlogistic cells by lysosomal enzymic and superoxide-anion modifications. Moreover DAR modifies phagocytic functions and the motility of cells. This paper is a contribution to the clarification of the last point, namely the effect of rhein on cell motility. It reports that in vitro no effect of R on random migration was found, but instead a double inhibiting effect on chemotaxis (i.e. a low-dosage and a high-dosage effect). Furthermore, R did not modify the inhibition or induce modification of chemotaxis by vinblastine. Finally R cancelled the stimulating effect of ionic potassium. The results thus indicate that R acts on the chemotaxis of the leukocytes with a complex action at different doses. The action mechanism is probably due to a membrane effect, since rhein (R) did not modify the chemotaxis-inhibiting activity of vinblastine but did interfere with the stimulating effect of K+.

Anthraquinones↗
PubMed1986

Severe pancytopenia due to copper deficiency. Case report.

A patient with copper deficiency and renal failure was suffering from pancytopenia. Marrow examination showed cytoplasmic and nuclear vacuolizations of the erythroid and myeloid series. These abnormalities disappeared after oral copper therapy. Possible mechanisms causing the pancytopenia are discussed. Copper supplementation is needed for patients receiving dietary changes.

Adult↗
PubMed1985

Modifications in the concentrations of circulating myoglobin after treatment with low doses of adriamycin.

The modifications in the concentration of circulating myoglobin have been studied by means of a radioimmunoassay in 15 cancer patients undergoing polychemotherapy including adriamycin. In 8 patients significant increases in myoglobin levels were found after injection of low doses of the drug (25-50 mg/m2). Moreover, a disturbance of the normal biorhythm of the protein was evident in 12 patients. Creatine kinase-MB was evaluated by means of a radioimmunoassay, but there was no relation between an increase in the isoenzyme and an increase in myoglobin. No ECG modifications were detected. These data indicate that the measurement of myoglobin may offer an indication of myocardial or skeletal muscle damage caused by adriamycin.

Adult↗