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Biomedical subjects

F Beatrice

Publications and source records attributed to F Beatrice.

36 records · Page 2Linked to original sources
PubMed1982

Production of leukocyte migration inhibition factor by lymphocytes of larynx cancer patients stimulated by laryngeal carcinoma solubilized membrane antigens.

The production of leukocyte migration inhibition factor (LIF) from lymphocytes after stimulation with 3 M KCl soluble tumor and normal mucosa extracts was investigated in 30 patients with laryngeal carcinoma at different development stages and in 30 normal donors. The experiments were performed in heterologous and autologous systems. In heterologous systems 3 M KCl tumor extracts induced LIF production by heterologous lymphocytes from patients in 91% of the cases, and normal mucosa extracts induced LIF production by heterologous lymphocytes from patients in 73% of the cases and from normal donors in 90% of the cases. In autologous systems 3 M KCl tumor extracts induced LIF production by autologous lymphocytes from the same patients in 65% of the cases, whereas the normal laryngeal mucosa extracts induced LIF production by the same autologous lymphocytes in the 6% of the cases. The high positivity percentage of the test in heterologous systems could be related to differences in the major histocompatibility complex. The 65% test positivity in autologous systems using tumor extracts could be related to the presence of tumor associated antigens.

Antigens, Neoplasm↗
PubMed1980

Effect of the cryopreservation on the production of leukocyte migration inhibition factor (LIF) by lymphocytes from normal volunteers and patients with laryngeal carcinomas.

The present paper reports the results of an investigation on the influence of cryopreservation methods of human lymphocytes on the leukocyte migration inhibition factor (LIF) production following PHA stimulation. The optimal freezing and thawing procedures, fetal calf serum and dimethyl sulphoxide concentrations are reported.

Blood Preservation↗
PubMedDate not supplied

Natural killer-mediated cytotoxicity in patients with laryngeal carcinoma.

Natural killer (NK)-mediated cytotoxicity against the K562 cell line was evaluated in normal subjects and in patients with laryngeal cancer. Results showed reduced lymphocyte cytotoxicity in patients with neoplastic disease and further reduction in NK activity following postoperative radiation therapy. The authors discuss the possibility of using this test in the immunological monitoring of patients with laryngeal carcinoma.

Cell Line↗
PubMedDate not supplied

Combined chemo-radiotherapy for stage IV undifferentiated nasopharyngeal carcinoma.

Undifferentiated nasopharyngeal carcinoma is a chemosensitive lesion, but its role in the management of local advanced disease is under investigation. Twenty-seven untreated stage IV undifferentiated nasopharyngeal carcinoma patients were treated with radiotherapy (median dose, 66.6 Gy, 1.8 Gy/day) and concomitant cisplatin (100 mg/m2 days 1, 22 and 43). After 4 weeks, patients received, every 4 weeks, 3 cycles with cisplatin (80 mg/m2 day 1) + 5-fluorouracil (1,000 mg/m2/day continuous infusion for 96 h). After radiotherapy, we observed 74% complete responses and 26% partial responses; after adjuvant chemotherapy 96% had a complete and 4% a partial response. After a median follow-up of 36 months, 81% of the patients were alive (70% with no evidence of disease). Four-year overall and disease-free survival was 70% and 60%, respectively. Concomitant chemotherapy plus radiotherapy was well tolerated, whereas adjuvant chemotherapy was more toxic. Long-term results were significantly better than those observed with radiotherapy alone.

Adult↗
PubMedDate not supplied

Concomitant chemoradiotherapy followed by adjuvant chemotherapy in parotid gland undifferentiated carcinoma.

AIMS AND BACKGROUND: Undifferentiated carcinoma of the parotid gland is a poor-prognosis lesion. Results in unresectable lesions, treated with radiotherapy alone, are very disappointing. METHODS: Six patients with T3-4 N0-1 inoperable lesions were treated with conventional radiotherapy (64-70 Gy, 2 Gy per fraction 5 times a week) and concomitant cisplatin (100 mg/m2, days 1, 22 and 43). Four weeks after radiotherapy, adjuvant chemotherapy (cisplatin, 80 mg/m2, day 1, + VP16, 100 mg/m2, days 1, 3 and 5, q = 3 weeks, for 3 cycles) was given. RESULTS: A median dose of 66 Gy (range, 64-70 Gy) was delivered, and all patients received 3 courses of cisplatin during radiotherapy. Five of 6 patients received all three chemotherapeutic adjuvant courses. Two months after the end of treatment, 3 CR (50%), 2 PR (33%) and 1 NC (16%) was observed. Median CR and PR duration was 26+ and 10 months, respectively. Median overall survival was 18 months. No severe acute or late toxicity was observed. CONCLUSIONS: Concomitant chemoradiotherapy followed by adjuvant chemotherapy in advanced unresectable undifferentiated parotid carcinoma is feasibile and well tolerated. The high percentage of long-lasting CR is encouraging.

Aged↗
PubMedDate not supplied

[Hearing disability in relation to audiometric threshold values: hypothesis on the determination of the onset of hearing loss].

BACKGROUND: The term auditory disability (AD) means the presence of auditory symptoms due to hearing loss. Until now the audiometric threshold cut-off between the presence or absence of AD was not clear. OBJECTIVES: In this study we attempted to define the value of audiometric threshold that could be considered as the limit between the presence or absence of weakness of auditory function, considered as the threshold as the 95th percentile of subjects that did not report AD. METHODS: The study group consisted of 1641 males, 891 normally hearing and 750 affected by noise-induced hearing loss (NIHL) Each subject was submitted to a questionnaire specifically created in order to determine the presence of AD. RESULTS: Statistical analysis confirmed the reliability of answers to items. AD was present in 577 subjects, not all affected by NIHL. Audiometric threshold and age correlated significatively with the degree of AD. CONCLUSIONS: The results obtained allowed the audiometric threshold values to be determined at the 95th percentile of subjects who did not report AD, in relation to the average of frequencies normally used in hearing loss evaluation.

Adolescent↗
PubMedDate not supplied

[Basal serum production of gamma-interferon in subjects with gramineous allergic rhinosinusitis, in carriers of cancer of the larynx and in normal control subjects: study with specific monoclonal antibody. Preliminary data].

The authors report preliminary data about IF-gamma (Interferon-gamma) basal serum production tested by monoclonal antibody in three groups of 25 patients: alpha (normal controls), beta (ragweed hayfever patients) and gamma (laryngeal carcinoma patients pT3-4, pN0----3, pM0 by UICC). The IF-gamma mean value in ragweed hayfever patients is about five times higher then normal controls. These data are highly statistically significative. Moreover no value of IF-gamma basal serum production is tested in laryngeal carcinoma patients. The data are discussed.

Adult↗
PubMedDate not supplied

[Does an immune reactivity to human laryngeal cancer exist?].

Authors reconsider the present state of knowledge about immunology of laryngeal cancer. Authors, besides, analyse, in a critical way, problems concerning immunological monitoring of patients bearing solid carcinomas. They conclude that, up to date, it is impossible to have a biological test which can concretely show the patient's immune state.

Antigen-Antibody Reactions↗
PubMedDate not supplied

Evidence of suppressor activity mediated by concanavalin-A stimulated cells towards aspecific LIF production.

Since the T-suppressor subpopulation is known to have a peculiar sensitivity to the mitogenic activity of Concanavalin-A we decided to verify whether in normal subjects it was possible to induce, by using the Con-A as a mitogenic factor, the proliferation of cells--possibly T-suppressor--which: a) had the property of adherence and b) show inhibitory activity toward LIF production. The statistical analysis of the results of our experimental patterns show that even if a difference between the suppression exerted on LIF production by Con-A stimulated versus not stimulated cells is present at any dilution, the level of significance rises from P less than 0.005 to P less than 0.0001 for the range of dilution 1:1/4 to 1:132, thus revealing that these experimental conditions are optimal for eliciting the blocking effect. The researches that are currently in progress in our laboratory seem to suggest that this experimental approach is of some value in characterizing the immunity pattern of laryngeal cancer patients and its evolution through the course of disease.

Cell Adhesion↗
PubMedDate not supplied

Evaluation of the immune responsiveness in laryngeal cancer patients with specific and aspecific tests: first results of follow-up in 80 cases.

We have evaluated in 80 patients suffering with laryngeal carcinoma their specific and aspecific immune responsiveness. We used the E rosette-test, the PHA-induced in vitro stimulation and the in vitro specific LIF production. We chose to use the LIF production test both for its marked sensitivity and because the lymphokines--which are soluble mediators--act as a "bridge" between the different fields of the immune response. Furthermore in our previous research, we observed that the specific LIF production was influenced in a negative way by adherent cells of the peripheral blood that could behave as blocking factors. For what attains the autologous LIF production, before and after the removal of adherent cells, our results show that the patients suffering with a cancer staged as T3T4N1N2N3 exhibit much more marked blocking phenomena than the patients with a cancer staged as T1T2T3N0. In fact the results demonstrate that in this second group only 62% of the cases, after the removal of the adherent cells, showed an increase of LIF production, while this percentage rose to 83% for the patients with a more advanced disease. A similar pattern was found in those patients who showed a recurrence two of three years after the primary disease.

Humans↗
PubMedDate not supplied

[Behavior of 8000 Hz audiometric threshold in chronic acoustic trauma].

The paper analyzes the 8 KHz threshold in subjects with noise-induced hearing loss. The study group consisted of 315 subjects occupationally exposed to noise and suffering from acoustic trauma, which was considered as a mean threshold of more than 25 dB at 2, 3 and 4 KHz. The mean shape of audiograms was characterized by a neurosensory deficit which was more elevated at 4 and 6 KHz whereas the threshold at 8 KHz was better. However, this pattern was found in 60% of the cases whereas in the remaining 40% the threshold at 8 KHz was worse. Subjects with the most impaired audiometric threshold at 8 KHz were mainly older, although cases were found in a low percentage of younger subjects. The results demonstrate that in individual cases it is impossible, on the basis of the shape of the audiogram, to establish whether a hearing loss is due to noise or age.

Adolescent↗
PubMedDate not supplied

[Relationship between auditory disability and auditory threshold in acute acoustic trauma: pilot study].

The aim of this study was to determine the relationship existing between auditory disability, according to WHO 1980, and pure tone audiometry threshold in noise induced hearing loss. Disability was evaluated on the basis of a questionnaire submitted to 286 subjects with acoustic trauma. Answers obtained were considered reliable on the basis of statistical analysis. Results showed that subjects with disability have an higher pure tone threshold than subjects without auditory disability. However it appears difficult to determine a precise threshold level ever which auditory disability may be considered present. The Authors suggest that this limit could be determined on the basis of pure tone threshold at the 10th centile of the group of subjects with disability or at the 90th centile of subjects without disability.

Adult↗
PubMedDate not supplied

[Determination of the onset of occupational hearing loss].

The paper examines the different aspects of the diagnosis of noise induced hearing disability and handicap. In the author's opinion a diagnosis of this kind can be made only if both an occupational acoustic trauma and a hearing disability or handicap are present. An occupational acoustic trauma can be considered to be present if the subject works in a noisy environment, if there are no auditory diseases other than of occupational origin, and if the audiometric threshold is neurosensorial, bilateral, more elevated at the higher frequencies and higher than the threshold of subjects on the same age but not exposed to industrial noise. On the other hand, the authors deem that there is a loss of hearing function when hearing disability and handicap are present according to the WHO definition. An experimental model is proposed that will establish the presence of hearing disability and handicap.

Audiometry↗
PubMedDate not supplied

Angiogenesis: prognostic significance in laryngeal cancer.

The aim of this study was to evaluate the role of angiogenesis in the progression of laryngeal squamous cell carcinoma (LSCC). We correlated disease-free survival with microvessel count (MC) in the hot spot areas of 97 randomly selected caucasian males with LSCC followed for 60 to 90 months after surgery with or without radiotherapy. The results obtained indicate that: a) MC higher than 130 microvessels/mm2 is a cut-off value that distinguished patients who relapsed during the follow up period; b) multivariated analysis indicates that MC (p < 0.00001) is an independent predictor of disease free-survival; c) multivariated analysis selectively done on cases with relapse demonstrates that MC correlates with the presence of metastasis (or/and M) with local relapse (T). We suggest that MC is useful in the assessment of prognosis in LSCC and probably will permit selection of patients that could benefit from anti-angiogenic therapy associated with chemotherapy and/or radiotherapy.

Adult↗