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Biomedical subjects

F C Bauer

Publications and source records attributed to F C Bauer.

15 recordsLinked to original sources
PubMed1987

High doses of the diphosphonate EHDP for the prevention of heterotopic ossification. An experimental and clinical study.

The effect of Ethane-1-Hydroxyl-1, 1-Diphosphonate (EHDP) on experimental and clinical heterotopic ossification was studied. Demineralized cortical bone matrix was implanted in the abdominal wall of three groups of adult male rats. Two groups received injections amounting to 5 and 20 mg/kg/day of EHDP, respectively, and a control group received placebo. The lower dose of EHDP had no effect on bone formation, whereas the higher dose resulted in a marked reduction in ash content and 45Ca uptake in the implants. Inhibitory effects on heterotopic bone and serum alkaline phosphatase activity were observed when treating with high doses (50 mg/kg/day) a paraplegic patient following resection of para-articular ossifications around the hips. The effect of EHDP on bone formation is interpreted as reflecting a dose-dependent interference with the deposition of hydroxyapatite crystals in the bone matrix.

Adult↗
PubMed1985

The osteo-inductive properties of bone matrix from rats pretreated with indomethacin.

Decalcified bone matrix pieces were prepared from growing rats treated for 3 weeks with subcutaneous injections of indomethacin 2 mg/kg/day or saline, and implanted into growing rats. Of the 16 recipients with 'saline implants', 8 received saline and another 8 indomethacin 2 mg/kg/day in subcutaneous injections. In another 16 recipients with 'indomethacin implants', 8 received saline and 8 indomethacin. As in previous studies, it was found that indomethacin exerted a mild inhibition of new bone formation expressed as decreased amount of ash weight in implants. The amount of ash weight and 45Ca specific activity of implants in recipients with implants from indomethacin-treated donor animals was not altered. These findings indicate that indomethacin is unable to modify the osteo-inductive properties of bone matrix.

Animals↗
PubMed1985

Influence of indomethacin on experimental bone metabolism in rats.

The effect of indomethacin on heterotopic and orthotopic bone formation in rats was analyzed with respect to dose and time of initiation of drug administration. Three weeks after implantation of demineralized bone matrix, the ash weight of implants from animals receiving the highest doses of indomethacin was approximately 25% lower than that of controls. The ash weight of implants was only affected in rats receiving indomethacin from the week before implantation or from the time of implantation. In a separate study, no effect on the rate of resorption measured by elimination of 3H-proline and 45Ca or on the amount of ash could be detected. Orthotopic bone remained unaffected by indomethacin treatment. The study showed that in order to inhibit experimental new bone formation, indomethacin must be present before or at the time of implantation of demineralized bone matrix.

Animals↗
PubMed1984

Effect of interferon on heterotopic new bone formation in mice.

To study the effect of interferon (IFN) on bone induction and new bone formation, heterotopic bone was induced by implanting pieces of demineralized bone matrix in soft tissue of mice. Mouse IFN was given as daily subcutaneous injections of 2 X 10(5) IU/mouse. Treatment was started one week before bone matrix implantation and continued until sacrifice. As a measure of chondrogenesis in the implants short-term incorporation of 35sulphur administered 11 days after implantation was measured. The amount of mineralized tissue formed in connection with the implant was estimated by 45calcium incorporation 20 days after implantation. There was no indication of a negative effect of IFN on bone induction, new bone formation, or metabolism of orthotopic bone. This is in agreement with our findings in the clinical use of IFN after reconstructive surgery in osteosarcoma.

Animals↗
PubMed1984

Krogius tenoplasty for recurrent dislocation of the patella. Failure associated with joint laxity.

Thirty-three patients treated with a Krogius tenoplasty for recurrent dislocation of the patella have been followed for an average of 3 years; the knees were classified according to Rünow. In 14 of 34 operated knees dislocations had recurred. Most of the recurrences were found in patients with generalized joint laxity, whereas only one of nine knees with only a high Insall index had redislocated. Patients with generalized joint laxity should not be treated with the Krogius tenoplasty as the sole procedure.

Adolescent↗
PubMed1984

Effect of the antineoplastic agent methotrexate on experimental heterotopic new bone formation in rats.

Heterotopic new bone formation was induced by implanting pieces of demineralized bone matrix in the abdominal wall of 22 growing Sprague-Dawley rats. The animals were divided into three groups and were given, 24 hr after initiation of the bone induction process, a single i.v. injection of methotrexate, 100 or 250 mg/kg body weight, or placebo, followed after 2 hr by leucovorin rescue. A slight and transient arrest in weight gain was noted in the methotrexate-treated animals. New bone formation during 3 weeks after implantation was analyzed by the amount of ash in implants, and as a measure of bone formation at the end of the experiment, short-time incorporation of 45Ca and [3H]proline was used. The ash content of implants was reduced by 56 and 68% in the two methotrexate groups. Uptake of both nucleotides was also greatly reduced in heterotopic bone, whereas metaphysial and diaphysial tibia and teeth were not affected. The results indicate a pronounced inhibition of methotrexate on bone induction, persisting for at least 3 weeks. Methotrexate reduces the bone-forming potential, with possible consequences for the success of limb-saving surgery and fracture healing.

Animals↗
PubMed1984

Effect of a diphosphonate on the osteoinductive activity of rat bone matrix.

Implants of demineralized allogeneic bone were investigated to determine the effects of ethane-1-hydroxy-1,1-diphosphonate (EHDP) on the osteoinductive effect of bone matrix. Implants of matrix prepared from bones of rats treated by EHDP (20 mg/kg/day) produced 40% less ash than implants of normal bone matrix. These results indicate that EHDP not only interferes with mineralization but also has a direct effect on protein metabolism of bone cells.

Animals↗
PubMed1984

Formation and resorption of bone induced by demineralized bone matrix implants in rats.

Demineralized bone matrix was implanted in the abdominal wall of growing rats. 45Ca and 3H-proline were injected 21 days after implantation, and groups of animals were killed at 30 minutes to 16 days. Implants, tibial metaphyses and diaphyses, and incisor teeth were assayed for ash weights and isotope content. The net increase in mineral content was largest in the early phase of bone formation. Three weeks after implantation, the mineral accretion was approximately 10% of tibial metaphyses, or 0.06 mg ash per milligram implanted bone matrix. In both these tissues, the net increase in ash weight was only one-fourth of the accretion because of the high rate of resorption.

Animals↗
PubMed1983

Experimentally induced heterotopic ossification in rats influenced by anti-inflammatory drugs.

This paper reports the effects of peroral administration of the two non-steroid anti-inflammatory drugs ibuprofen and flubiprofen on orthotopic and induced heterotopic bone. The treatment induced no effects on the mineral or hydroxyproline contents of femur compared with a control group. Nor could any generalized toxic effects of the treatment be detected with respect to bodyweights or serum values of calcium, phosphate or albumin. Treatment with the drugs was commenced one week before initiating the heterotopic bone formation by implanting pieces of demineralized bone matrix in the abdominal muscle of growing rats. A significant decrease in specific amounts of calcium and phosphate was noted in the induced new-formed bone 4 weeks after implantation, whereas no such effect was apparent 2 weeks after implantation.

Animals↗
PubMed1983

Growth characteristics of human malignant tumours and cell lines in serial passages in nude mice.

Two osteosarcoma cell lines, one fibrosarcoma and one undifferentiated mesenchymal tumour obtained at operation, all of human origin, were transplanted in serial passages in athymic nude mice. The growth rate was analysed and the morphologic appearance compared both with the original tumours and after each passage. All tumours retained their morphologic characteristics during the transplantation and passaging in the nude mice with only minor alterations in structure. The growth rates of the tumours showed great variability even in the same passage of each tumour, although the growth rates became somewhat more regular and predictable after some passages.

Adult↗
PubMed1981

Bone induction by Ewing's sarcoma. Transplantation into athymic nude mice.

Fresh specimens from two cases of Ewing's sarcoma were implanted in thigh muscles of nude athymic mice. Ewing's sarcomas induced differentiation of mouse-derived new bone in the same fashion as osteogenic sarcomas and chondrosarcomas. Control transplants of eight primary nonosseous and metastatic tumors did not induce bone formation. The osteoinductive action of Ewing's sarcoma is submitted as evidence of its origin from an osteogenic cell rather than from a hematopoietic cell line.

Animals↗
PubMed1981

The reaction of giant cell tumors of bone to transplantation into athymic nude mice: including observations on osteoinduction in the host bed.

Fresh biopsy specimens of three typical human giant cell tumors (GCT) were transplanted into athymic mice. The transplants were encapsulated in a host bed-derived fibrous connective tissue envelope. Multinucleated giant cells and mononucleated tumor cells survived chiefly on the vascularized surfaces of the inside of the envelope. Fibroblasts proliferated and filled the interior of the transplants where vascularization was relatively insufficient. Since mitotic figures were absent in multinucleated cells, the question arises whether only the mononuclear stroma cells are neoplastic in nature. Multinucleated cells occasionally showed endocytosed pyknotic cells, and survived transplantation for as long as 35 days. When the original tumor contained islands of osteoid and bone cells, the transplants induced differentiation of mouse host bed connective tissue into normal new bone, including bone marrow.

Adult↗
PubMedDate not supplied

Human osteosarcoma-derived soluble bone morphogenetic protein.

A bone morphogenetic protein (BMP) fraction obtained from the organic matrix of dentin is extractable from human osteosarcoma. A fresh en-bloc specimen excised from the femur of a 16-year-old boy was extracted with 4 M guanidine hydrochloride. The GuHCl-soluble, water-insoluble protein fraction induced new bone formation when implanted into the thigh muscles of athymic nude mice.

Animals↗
PubMedDate not supplied

Giant cell tumor with viral-like intranuclear inclusions associated with Paget's disease.

A case of giant cell tumor (GCT) of the distal femur in a 56-year-old man is reported in association with Paget's disease. The case is unique in that is is the first report of GCT arising in a patient with this disease in which the neoplasm arose in a bone that did not show radiologic evidence of Paget's disease. This case also represents the first example of such an association in which viral-like inclusion bodies are seen in the osteoclast of the tumor. The fact that similar inclusion bodies are seen in the osteoclast of patients with Paget's disease and in their GCT implies a cause-and-effect etiologic relationship rather than mere coincidence. The implications are that a slow-virus infection is involved in the pathogenesis of Paget's disease and in giant cell tumors occurring in patients with this disease.

Cell Nucleus↗