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F Cappello

Publications and source records attributed to F Cappello.

At least 37 records · Page 2Linked to original sources
PubMed2001

Determination of coreceptor usage of human immunodeficiency virus type 1 from patient plasma samples by using a recombinant phenotypic assay.

We developed a recombinant virus technique to determine the coreceptor usage of human immunodeficiency virus type 1 (HIV-1) from plasma samples, the source expected to represent the most actively replicating virus population in infected subjects. This method is not subject to selective bias associated with virus isolation in culture, a step required for conventional tropism determination procedures. The addition of a simple subcloning step allowed semiquantitative evaluation of virus populations with a different coreceptor (CCR5 or CXCR4) usage specificity present in each plasma sample. This procedure detected mixtures of CCR5- and CXCR4-exclusive virus populations as well as dualtropic viral variants, in variable proportions. Sequence analysis of dualtropic clones indicated that changes in the V3 loop are necessary for the use of CXCR4 as a coreceptor, but the overall context of the V1-V3 region is important to preserve the capacity to use CCR5. This convenient technique can greatly assist the study of virus evolution and compartmentalization in infected individuals.

Acquired Immunodeficiency Syndrome↗
PubMed2001

[Popliteal trauma due to posterior dislocation of the knee].

Popliteal artery injuries have an incidence ranging between 5% and 19% of all traumatic arteria lesions. A high index of amputation, when associated with lesions of the infrapopliteal branches, and a 4-5% overall mortality are recorded in these conditions. Three patients with popliteal artery injury due to posterior knee dislocation were operated upon in our department during the last 12 months. In one case, the physical examination revealed a warm limb, with peripheral pulse; angiography showed a pseudoaneurysm of the popliteal artery with intimal dissection and partial thrombosis. In the last two cases an acute ischemia of the limb was present; in one case the angiographic study showed a complete transection of the artery, while in the second case an obstruction of popliteal artery. In all cases the dislocation was corrected and a the reconstruction carried out by inverted autologus saphenous vein with termino-terminal anastomosis. In all cases a good patency of the popliteal reconstruction was achieved with limb salvage. Prognosis of popliteal injuries is related to an early diagnosis and they should be suspected even in absence of overt signs of acute ischemia. Surgical timing cannot be the same in all cases. The need for a preliminary orthopedic phase with its modality must be established case by case, relating to the severity of ischemia, to the time elapsed between trauma and surgery, to the peculiarities of skeleton and joints lesions.

Acute Disease↗
PubMed2001

[Elective and emergency surgical treatment of popliteal aneurysms. Indications and results].

BACKGROUND: The aim of this paper is to evaluate the long term results related to surgical technique and to prosthetic material in planned and emergency conditions. METHODS: From January 1990 to December 1999, fourty-five patients with popliteal aneurysms were observed. Eighteen patients (40%) were asymptomatic; eleven (24.4%) suffered from claudicatio; six had an acute ischemia (13.3%) and four presented (8.9%) clinical signs of rest pain; in four cases (8.9%) symptoms were related to venous compression and to rupture in two (4.4%). Diagnosis was obtained by ultrasounds, angiography and CT-scan. All patients were operated on and in four cases (8.9%) the procedure involved both legs. The prosthetic material was reversed autologous saphenous vein (ASV) in 30 patients (61.2%), PTFE-EXS-TW in 16 (32.6%), homologous vein in one (2%), composite graft in one (2%), and a Dacron in the last one (2%). RESULTS: Six patients died for causes not related to the operation and eight bypasses (18.2%) became occluded. Amputation was needed in two patient, in relation to late bypass occlusion. In one cases an amputation was carried out for acute thrombosis of the contralateral aneurysm which was not treated for patients refusal. The long term patency rate was 81.8%. CONCLUSIONS: Popliteal aneurysms must be considered for reconstructive surgery, also when asymptomatic and with a diameter over 2 cm. The routinely use of the ASV improves the long-term patency rate. Best long term results are obtained in elective surgery.

Aneurysm↗
PubMed2001

[Indication for short stripping of the great saphenous vein. Results and indications].

BACKGROUND: The aim of surgical therapy of varicose veins is the elimination of reflux from the deep to superficial system at the saphenous crosse and perforant vessel and conservation of the superficial venous system due to possible surgical procedures for arterial revascularization. This latter condition leads to an extension of indications for short stripping procedures, although the venous distal segment may undergo hypoplastic degeneration not compatible for revascularization purposes. Another important reason is the minor incidence of neurologic complication due to saphenous nerve lesion which may occur during long saphenous stripping. METHODS: From January 1994 to June 1999, we considered 233 patients (182 women, 51 men); 180 cases underwent long saphenous stripping procedures, whereas 53 a short stripping of GSV. The incidence of neurologic complications of the saphenous nerve were recorded in 11.6% of the patients treated with the standard procedure, whereas no such complication was observed in all cases treated with the short stripping procedure. RESULTS: ECD follow-up performed for a period of three months from the surgical procedure revealed the patency of the residual saphenous vein, with a minimum diameter of 3 mm, in 28 patients (56.6%). CONCLUSIONS: Our opinion is to extend the indication for short stripping of the saphenous vein to all cases where the distal saphenous trunk is not involved, when the ECD shows a pathological ostial reflux, a truncular reflux limited to the thigh, which may be associated with incontinence of the perforant vein of Dodd.

Female↗
PubMed2000

Mature teratoma of the uterine corpus with thyroid differentiation.

A case of a clinically silent mature teratoma of the uterine corpus is reported. A 55-year-old woman presented with multiple uterine leiomyomas. The discovery was incidental, because the patient was asymptomatic. Macroscopically, a colloid-hemorrhagic-looking nodule was present. Histologic and immunohistochemical studies showed that this tumor was a small thyroid mass. Key words:,

Calcitonin↗
PubMed2000

Involvement of caspase-3 and GD3 ganglioside in ceramide-induced apoptosis in Farber disease.

Farber's disease (FD) is a rare genetic disorder caused by ceramidase deficiency, which results in ceramide accumulation in lung, liver, colon, skeletal muscle, cartilage, and bone. Although this disease has been symptomatically characterized, little is known about its molecular pathogenetic process. Because recent studies reported that ceramide accumulation induces GD3 ganglioside formation and apoptosis, we investigated, in tissue obtained via colonoscopy from seriously involved patients, the possible involvement of ceramide in FD colonocyte destruction. Histochemical and TUNEL analyses of paraffin-embedded sections revealed that 45 +/- 4.3% of FD colonocytes showed morphological signs of apoptosis compared with the 8 +/- 2.3% of constitutive epithelial cell death. Importantly, immunohistochemical study for pro-apoptotic factors showed that GD3 accumulation co-localized with active caspase-3 and cleaved K18 in FD colon tissue. These findings provide evidence for a role of the apoptotic ceramide pathway in the pathogenesis of FD.

Adult↗
PubMed2000

[Breast cancer and lymph node metastasis: relation of lymph node dimensions and metastasis].

The Authors have examined 1159 lymph nodes obtained from 64 cases of breast carcinoma to value the presence of a correlation between the insurgence of metastases and lymph node dimensions. Metastases were more frequent in larger lymph nodes. Nevertheless, metastases were also present in small (< 5 mm) or very small (< 2 mm) lymph nodes. This indicates the importance to carefully examine small lymph nodes during gross examination and to obtain multiple sections from them.

Breast Neoplasms↗
PubMed1999

Defective apoptosis as potential mechanism in the tumorogenesis of myelolipoma.

Apoptosis is considered an important mechanism of selective deletion that occurs during hematopoiesis. Myelolipoma is a rare benign tumor composed of adipose tissue and hematopoietic cells. The pathogenesis of this benign tumor is still unclear. Analysing the structural levels and apoptosis of normal human bone marrow (NHBM) and human myelolipoma (HM), the apoptotic events resulted abundantly present in NHBM compared to HM, which showed a small number of apoptotic cells. By contrast, Fas expression was strongly present both in NHBM and HM. These findings suggest that an altered function of Fas in myelolipoma is not able to trigger the apoptotic machinery. In conclusion, we hypothesize that drastic reduction of apoptosis in myelolipoma can be considered one of the growth regulatory mechanisms.

Adrenal Gland Neoplasms↗
PubMed1999

Involvement of Fas/FasL system in the pathogenesis of autoimmune diseases and Wilson's disease.

The interaction of Fas with FasL has been demonstrated to be implicated in the pathogenesis of several autoimmune and liver diseases. Recently, attention has been focused on the hypothesis that thyrocytes and beta cells undergo massive Fas/FasL-mediated apoptosis during autoimmune response. Similarly, hepatocyte cell death occurring following copper accumulation points towards the same mechanism.

Diabetes Mellitus, Type 1↗
PubMed1992

[Project for a computerized system of information on pregnancy and labor (GEST-PARTO)].

The Obstetrics Division of Schio Hospital has introduced the computerised management of pregnancy and birth in order to obtain standard and complete data on both pregnancy, wherever the woman is followed, and birth, whenever this occurs and by which ever team the woman is assisted. Computerised management is possible on account of the low costs, the availability of large ranges of software and the ability to transfer data. It is based on the presumption that on being admitted to hospital all women must possess a clinical record of their pregnancy stating the obstetrical risk. The programme GEST-PART contains a 6-letter password which gives access to the utility menu.

Diagnosis, Computer-Assisted↗
PubMed1991

[Effect of of cervical antitumoral immunity activation on CIN].

The effects on priming and strengthening immunitary action in the uterine portio were assessed in CIN 2 and CIN 3. Use was made of: solution of BCG 0.01 ml (1/10 of a vaccination dose) repeated if necessary after 5 days, diluted with 100,0000 IU of beta-interferon injected radially into the quadrant of the portio; 1.000.000 IU of beta-interferon intradermally for a further 4 days, to be repeated on the basis of local findings. 20 cases of CIN 2 and CIN 3 were analysed. The immediate results are characterised by complete desquamation of the pathological epithelium, intensive lymphoplasmacellular and macrophagic reaction with the presence of lymphofollicles. In two cases (10%), subsequent conisation was carried out because of persisting CIN foci. Given the minimal dose of BCG and interferon, no complications were observed. The combined or sequential action of the two drugs in assessed as well as the speed of spontaneous restitutio ad integrum until a longer follow-up finally confirms results obtained.

Adult↗
PubMed1990

Thymostimuline and uterine cancer satellite lymph nodes. Morphometric comparison between an 8-day-before-surgery and a 14-day-before-surgery single treatment.

The Authors analyse the functional areas (cortical, paracortical, germinal center, medullary, histiocytosis of the sinuses) of regional lymph nodes in women having uterine carcinoma and previously treated with Thymostimuline, and who were operated on respectively 8 and 14 days after inoculation. They also evaluate the degree of significance when compared with a control group treated locally with a physiological solution. The number of lymph nodes examined is at least 5, and the stereological analysis was carried out with the use of a grid: 200 points, actual area 200 cm2, effective area at the level of the preparation 0.32 mm2 (250 X). The Authors examine the results obtained after 8 days' action by Thymostimuline, and, after 14 days, the difference between the 8-day-after and the 14-day-after administration results, and those between the cervical and the endometrial. 4 women with uterine cancer (Stages I and II) and treated with the same doses of physiological saline solution were used as controls.

Carcinoma, Squamous Cell↗
PubMed1989

Morphometric analysis of peritumoral lymph nodes in patients operated on for uterine cancer, locally treated with a thymic extract.

By means of a morphometric analysis, the authors have evaluated the structure and dimensions of the lymph node functional areas (cortical, medullary, paracortical, histiocytosis of the sinuses, germinal centres) in regional nodes of women with carcinoma of the uterus in the 1st and 2nd stages. Twenty patients were treated 8 days before surgery with a dose of 1.5 mg kg-1 body weight of thymic hormone directly into the uterine portio. Ten patients, on the other hand, were injected with physiological saline solution. The quantitative and qualitative results show that in peritumoral lymph nodes of women treated with thymostimolin there is a marked increase, statistically significant (P less than 0.001), of the paracortical zone and of the number of germinal centres.

Female↗
PubMed1989

Steriological analysis of peritumoral lymph nodes in patients operated for cervical and endometrial cancer after being treated with thymostimulin.

We have evaluated, by means of stereological analysis, the dimensions of the lymph node functional areas (cortical, medullary, paracortical, histiocytosis of the sinuses, germinal centers) in regional lymph nodes of women with cervical cancer (15 women) and endometrial carcinoma (6 women) in Ist and IInd stage, treated 8 days before surgery with a dose of 1-1.5 mg/kg of body weight of thymostimulin directly into the uterine cervix. 4 women with cervical and endometrial cancer (stages I and II) and treated with the same doses of physiological saline solution were used as controls. The quantitative results show that in the lymph nodes of women treated with thymostimulin there is a statistically significant marked increase (P less than 0.001), of the paracortical areas.

Female↗
PubMed1989

Melanoma relapse of the upper third of the vagina treated with BCG and beta-interferon.

Faced with a vaginal melanoma relapse in an old cardiac patient operated in May 1985 for melanoma in the upper 3rd of the vagina, we had to solve a therapeutic problem in view of the impossibility of excising a lesion larger than the preceding one and because the patient refused every type of surgery. So we then chose the combined treatment of intralesional BCG and intravenous beta-Interferon. Monthly intravenous administration of beta-Interferon has reached a total of 8,000,000 I/U, while the BCG has been inoculated intralesionally in the submucosa in doses of 0.1 ml after observing the negative result of the PPD test. Our aim was to achieve double immunological stimulation by combining two drugs that are often used separately with satisfactory results. Before and after every therapy cycle, the immunological status was established, and showed a small but constant and permanent increase in T-lymphocytes, both helper and suppressor. Histological results before and after therapy and immunological responses are reported, and the case distinguishes itself by its brilliant results.

Aged↗