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Biomedical subjects

F Cho

Publications and source records attributed to F Cho.

At least 91 records · Page 5Linked to original sources
PubMed1978

Bone growth aud development of secondary ossification centers of extremities in the cynomolgus monkey (Macaca fascicularis).

The development of so-called long bones in the extremity has been studied roentgenographically in forty-seven males and fifty-one females cynomolgus monkeys bred and reared at the National Institute of Health. The age of the females ranged from five months to eight years and nine months, and that of the males was from four months to seven years. In addition, the fetuses of six to twenty weeks of gestation age were examined for the time of appearance of ossification centers. As the biological parameters concerning body growth, the body weight and the bone length were measured and the secondary ossification centers were scrutinized and assessed the maturity process on the basis of the criteria that divided the state into eleven stages. Also the allometric analyses of body weight against bone length was conducted. Most of the secondary ossification centers except the proximal fibulal epiphysis appeared during the period from the prenatal stage (15-20 weeks of gestationage) to the postnatal one (several months of age). From four to five months of age, many ossification centers had developed to some extent. But, the appearance of proximal fibulal epiphysis was delayed and often lacking until 10 months of age in female and one year and three months of age in male. The earliest epiphyseal fusion was observed at the distal humeral epiphysis in both sexes. The latest epiphyseal fusion was observed at the distal ulnal epiphysis in both sexes and at the distal ulnal and radial epiphyses in female. From this study, the time of fusion was at five and three guarters years of age in females and at six and a half years of age in males. As a result, it is suggested that the estimation of animal's age might be put to practical use by introducing the assessing method that the score was given from the observation of the secondary ossification center.

Animals↗
PubMed1978

Breeding of cynomolgus monkeys through successive generations by indoor cage system.

Vital statistics on the breeding through successive generations were presented for the cynomolgus monkey colony of NIH, Tokyo. The results of this retrospective survey clearly demonstrated the third (F2) and the fourth (F3) generations could be bred and reared successfully by the indoor caged-breeding system in which either individual timed-mating or group mating procedure was adopted. Several important and difficult problems involved in the production of successive generations of the cynomolgus monkey by our breeding system were discussed from the standpoint of laboratory animal science.

Age Factors↗
PubMed1976

[Nursing procedures for cynomolgus monkey infants (author's transl)].

This paper describes the results of the artificial nursing of 8 infant cynomolgus monkeys (Macaca fascicularis) weighing 230-350 g at birth. The infants were separated from their mothers just after or 2 days after birth and were kept in a specially deviced small cage (Photo 1 & 2). They were fed on 6.4-16.6% solution of a commercially available powdered milk for the human baby. Apple juice also was given to them from the 3rd or 4th week on. In about the 6th week, the infants received solid foods such as small pieces of apple, biscuit and monkey chow. The main part of the dietary regimen was changed to the solid diet around the 12th week. The volume of milk taken was recorded every day, and the daily intake ff three major nutrients was estimated with the milk intake (Fig. 1,2,5,6). The body weights were between 600 and 750 g on the day of weaning, that is at about 12 weeks of age, except No. 2 infant showing a slight growth retardation (Fig.4). Their physical growth can be judged as normal as that of breast-nursed infants of the saame species from our laboratory. On the basis of these results, an example of the artificial feeding schedule for the infant cynomolgus monkeys was introduced (Fig. 7).

Animal Feed↗
PubMed1976

Chemical composition of cynomolgus monkey milk.

Milk samples from eight cynomolgus monkeys whose lactation period ranged 44-119 days were investigated for their gross chemical composition such as total solids, crude protein, lipids, lactose and ash. Nitrogen distribution, compositions of fatty acids, amino acids and triglycerides as well as major minerals were also determined. These analytical data were compared with those reported for man, simian and cow, and cynomolgus monkey milk was found to have close similarities to human milk.

Amino Acids↗
PubMed1976

Oral glucose tolerance test in the cynomolgus monkey (Macaca fascicularis).

The glucose tolerance test was simplified so that it could be performed on more than one cynomolgus monkey (Macaca fascicularis) at a time. Glucose was administered orally in a dose of 2 g/kg body weight as a 50% aqueous solution, and blood samples were taken just before and 30 minutes and 150 minutes after glucose administration. The simplified test was conducted on a total of 93 cynomolgus monkeys. Glucose tolerance curves obtained by this method were classified into three patterns with regard to the increasing rate of serum glucose concentration per minute and the decreasing rate. Seventy-one monkeys showed the first pattern characterized by an increasing rate of 1.0 mg/min or more and a decreasing rate of 0.2 mg/min or more. The second pattern showing an increasing rate of 1.0 mg/min or more and a decreasing rate of less than 0.2 mg/min was obtained with 10 monkeys. The remaining 12 monkeys exhibited the third pattern in which the increasing rate and the decreasing rate were less than 1.0 mg/min and 0.2 mg/min, respectively. The first pattern is considered to be normal glucose tolerance, while the second and third patterns are regarded as abnormal ones. The incidence of the abnormal patterns was significantly higher in wild-imported cynomolgus monkeys than in laboratory bred monkeys.

Animals↗