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Biomedical subjects

F Domínguez

Publications and source records attributed to F Domínguez.

69 records · Page 4Linked to original sources
PubMed1981

Tryptophan overload in the pregnant rat: effect on brain amino acid levels in in vitro protein synthesis.

The concentration of most amino acids was higher in the brains of 19- and 21-day rat fetuses than in their respective mothers. After an intraperitoneal load of tryptophan to the mother, the intracerebral concentration of several amino acids (including leucine) decreased not only in the mothers, but also in their fetuses. The in vitro incorporation of [3H]leucine into proteins in brain postmitochondrial supernatant fractions was enhanced in both the mothers and fetuses after tryptophan administration, but this effect disappeared when protein synthesis was calculated by using specific activities corrected for the amount of unlabeled leucine in the preparation. By this criterion, protein synthesis activity appeared similar in the brains of 19- and 21-day pregnant rats but was higher in their fetuses, especially in the 21-day subjects. Thus, protein synthesis in the brain was not altered by marked changes in the amino acid pool and more profound and prolonged metabolic disturbances must occur to cause permanent damage in the developing brain.

Amino Acids↗
PubMed1979

[Radiological manifestations of Meckel's diverticulum (author's transl)].

Multiple clinical and radiological manifestations of Meckel's diverticulum are commented, presenting four cases with acute abdominal manifestations and one with chronic symptomatology, which were selected from a series of nineteen cases that were surgically verified. The authors realize a critique of the radiological techniques in the literature of the suspected cases of said entity.

Child↗
PubMed1978

[Experience and application of diagnostic pneumoperitoneum in the anomalies in the umbilical region (author's transl)].

By means of 30 pneumoperitoneograms performed "postmortem" and five performed for diagnosis, the exploratory technique, radiologic anatomy of umbilical region, and the usefulness of the process in the diagnosis in children are discussed. Pneumoperitoneum may be indicated in diagnosis of bleeding Meckel's diverticulum, in exclusion or confirmation of remnants of the omphalomesenteric duct in cronically moist lesions of the umbilicus resistent to symptomatic treatment, in cases of non-communicating urachal cysts which can not be diagnosed by cystogram, and in the differential diagnosis of abdominal tumors related to the umbilical region.

Child, Preschool↗
PubMedDate not supplied

Human endometrial receptivity: a genomic approach.

The endometrium is a specialized tissue, hormonally-regulated, that is non-adhesive for embryos throughout most of the menstrual cycle in humans and other primates. Thus, endometrial receptivity is a self-limited period in which the endometrial epithelium (EE) acquires a functional and transient ovarian steroid-dependent status. The luminal EE acquires the ability to adhere (receptivity) the developing human blastocyst during this period due mainly to the presence of progesterone after appropriate 17beta-oestradiol priming. This status is a key element for embryonic implantation and appears to be closely associated with morphological and biochemical changes of EE cells. This specific time window is thought to be open after 4-5 days and closes after 9-10 days of progesterone production or administration, creating a physiological window of receptivity limited to days 19-24 of the menstrual cycle in humans. The scientific knowledge of the endometrial receptivity process is fundamental for the understanding of the human reproduction, but, so far, none of the proposed biochemical markers for endometrial receptivity have been proved clinically useful. In this work new strategies are presented based on molecular biology technologies that aim to clarify the fragmented information in this field using differential display, quantitative PCR and cDNA microarray analysis of endometrial epithelial-derived cell lines and endometrial samples to investigate the hierarchy at the mRNA level of molecules implicated in the process of endometrial receptivity.

Blastocyst↗
PubMedDate not supplied

Fine needle aspiration of breast masses. An analysis of 1,398 patients in a community hospital.

OBJECTIVE: To determine the diagnostic efficacy of breast fine needle aspiration (FNA) in the routine practice of a community hospital. STUDY DESIGN: The study group consisted of 1,398 consecutive FNA biopsies of the female breast performed at our institution during a five-year period; 450 (32%) had histologic follow-up. Both FNA and the histologic diagnosis were compared in order to establish the accuracy, efficiency and safety of the FNA breast technique. RESULTS: The sensitivity of the FNA procedure was 93.49% and specificity 95.73%, with a predictive value for a positive diagnosis of 93.49% and for a negative diagnosis, 95.73%. The overall diagnostic accuracy was 94.84%, and the sensitivity of cytologic diagnosis was 98.75%. The false negative and false positive rates were 2.5%, including 10 cases with suspicious diagnoses and only 1 false positive diagnosis (0.23%). The false positive and false negative cases were recorded as having no effect or a minimal effect on patient management, and no mastectomy was performed because of this FNA diagnosis. CONCLUSION: FNA breast biopsy is an efficient tool for diagnosis when performed by surgical pathologists with cytologic training but not exclusively dedicated to cytopathology.

Abscess↗
PubMedDate not supplied

[Analysis of the echocardiographic findings in young patients with cerebral ischemia].

INTRODUCTION: When we consider the importance of cardioembolism in the aetiology of cerebral ischaemia in young adults, echocardiography, both transthoracic and transesophageal, becomes of increasing diagnosis importance. We analyze its usefulness and contribution to the reduction in the number of cerebral ischemias of unknown origin. PATIENTS AND METHODS: We selected persons aged between 14 and 45 years who had had cerebral ischaemia during the period between January 1991 and April 1998. The protocol for the diagnosis of stroke in young persons was applied to all of them together with transthoracic echocardiography whenever there was the possibility of embologenous cardiopathy followed by transesophageal echocardiography when the transthoracic echocardiography was not diagnostic. RESULTS: In 114 identified cases, 98 transthoracic echocardiographs were done, of which 14 were diagnostic, together with 32 transesophagic echocardiograms, which showed nine cases of embologenous cardiopathy not detected on transthoracic echography. The most frequent diagnoses were: prolapsed mitral valve (14); patent foramen ovale (8); mitral stenosis (5) and interauricular septal aneurysm (4). A total of 31 patients were diagnosed as having a previously unrecognized cardioembolic origin of their illness. CONCLUSIONS: The diagnostic usefulness of transthoracic echography was 22.45% (9.18% were false negatives), and 28.12% for transesophagic echocardiography. These reduced the diagnosis of ischaemia of unknown origin by 32.69% and 20% respectively. Besides, in 75% of the patients with a patent foramen ovale and 100% of those with interauricular septal aneurysms, no other possible cause for their condition was found. Transthoracic echocardiography is a technique to be recommended in all cases of cerebral ischaemia of unknown origin in young adults. Its usefulness may be enhanced by transesophagic ultrasonography when diagnosis cannot be definitely made on transthoracic echosonography alone.

Acute Disease↗
PubMedDate not supplied

Secretory carcinoma of the breast. Report of a case with diagnosis by fine needle aspiration.

We report a case of secretory carcinoma of the breast in a 63-year-old woman studied by fine needle aspiration (FNA). The cytologic features included solid nests, cohesive sheets and isolated tumor cells with intracytoplasmic vacuolization and signet-ring-like forms. Although secretory carcinoma is an unusual breast tumor, especially in adults, the cellular morphology was distinctive on FNA. This could permit the preoperative diagnosis of secretory carcinoma and the planning of optimal surgical therapy prior to an intervention.

Biopsy, Needle↗
PubMedDate not supplied

[Massive thrombosis of the left atrium. Surgical experience with a series of 117 patients].

From 1975 to 1988 we have operated 117 patients with left arterial thrombosis associated with rheumatic mitral valve disease. Seventy-seven were female and 40 male, with ages ranging from 22 to 69 years. In 75 cases (64.1%) the valvular lesion was mitral stenosis. Embolic antecedents were present in 38 cases (32.4%) and 95 patients (81.1%) were in class III or IV of the NYHA functional classification. In 48 cases we performed a mitral commissurotomy and in 51 cases mitral valve replacement, associated to left artrial thrombectomy. In the remaining 18 patients we made other valve procedures. The hospital mortality was 15 cases (12.8%), eight because low cardiac output, four because severe brain injury and three because posterior atrioventricular sulcus disruption. In 41.1% of the survivors there was serious hospital complications, standing out the incidence of 8 cases of transient neurologic accidents. We have followed 98 of the 102 hospital survivors between 10 and 140 months (mean 57 months). Three patients died in the follow-up, two of them during a reintervention because bioprosthesis disfunction and the third one during a reintervention because prosthetic infective endocarditis. Nine additional patients were reoperated because recidivant valvular lesions or because prosthetic disfunction, and two patients suffered embolic events during the follow-up. The antithrombotic therapy was abandoned in 19.6% of patients. At present 73.6% are in functional class I and 26.3% in class II. The association of left atrial thrombosis with with mitral valve disease induce a surgical morbimortality greater than usual for isolated valvular lesions, being mandatory a watchfull surgical technic.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMedDate not supplied

[Some additional facts about thymus (author's transl)].

Three cases are presented of eventual diagnostic errors concerning the thymus. In the first case right aberrating lobe appeared radiologically as a tumour of posterior mediastinum. In the second case, the large thymic malleability, enabled it to invade medium mediastinum and to show an extraordinary variability in shape when in decubitus. Finally, a post surgical hematomata, in a patient affected by a cyanogen cardiopathy actually showed some problems of radiological diagnosis with the characteristic thymus regeneration.

Diagnostic Errors↗