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Biomedical subjects

F Fakan

Publications and source records attributed to F Fakan.

At least 73 records · Page 4Linked to original sources

[The significance of enzyme cytochemical methods in the classification of hemoblastoses].

Selected cytoenzymological methods together with the PAS reaction often permit more precise identifying normal and tumorous blood cells and thus determining the type of hemoblastosis. The cells of the myeloid neutrophil series are characterized by the activity of peroxidase and naphtol-AS-D-chloracetate esterase, monocytic elements by the activity of a nonspecific esterase sensitive to fluoride, the tumorous cells of the red series by the PAS positivity and paranuclear positivity of acid phosphatase. Some tumorous lymphoblasts have gross PAS--positive granules in the plasma, others, probably of T-origin, paranuclear focal positivity of acid phosphatase. Hematological classification of acute hemoblastoses does not exactly correspond to Löffler's cytochemical classification; of diagnostic and therapeutically considerable importance is the synthetic evaluation of the hematological finding and of interpretive possibilities of individual cytochemical methods.

Acid Phosphatase↗

[Chronic granulomatosis in childhood].

In a boy aged 8 years suffering from chronic granulomatosis of childhood necrotizing pneumonie and small pulmonary tuberculoid granulomas containing filaments of moulds were found at autopsy. Necrotizing leucocytic granulomas were present in the liver, spleen and the lymph nodes. All the organs showed aggregates of histiocytes containing yellowish cytoplasmic deposits of lipogment surrounded by a high acid phosphatase activity. The NBT-reduction leucocytic tests was repeatedly negative in vivo. The activities of NADH- and NADPH-tetrazolium reductase and succinate dehydrogenase in the tissues were histochemically normal.

Age Factors↗

[Generalized varicella and herpes].

Generalized varicella was recognized as the cause of death in three children previously treated with corticoids, whereas generalized herpes simplex was the cause of death in one immature neonate. In all the cases the findings in internal organs were similar. They included disseminated necroses and mucous ulcers with a leucohistiocytic inflammatory reaction, karyorrhectic disintegration of nuclei and the presence of intranuclear inclusions of the Cowdry A type.

Adrenal Glands↗

[Myelomonocytic chloroleukemia. Histochemical evaluation of two observations].

A myelosarcoma was diagnosed in the bioptic excision of an eight year old boy's middle ear. An autopsy of this case as well as that of a 47 year old man revealed the presence of tumoriform chloroleukaemia. The histochemical positivity of naphthol AS-D-chloracetate-esterase and NaF-sensitive non-specific esterase showed the simultaneous double differentiation of tumour cells thus serving as evidence of their myelomonocytic nature. The first observation also revealed the presence of atypical eosinophil leucocytes with PAS-positive, naftol AS-D-chloracetate-esterase-negative granules. Both observations brought evidence of the reticular as well as tumour cells showing signs of erythrocytophagia which may have a share in the development of the tumour tissue green coloration.

Child↗

Protein histochemistry methods for colloid identification to metastases of thyroid carcinoma.

Evidence for a thyroid origin of tumorous metastases may be facilitated by a histochemical determination of colloid. It is supported by the presence of proteins, demonstrable especially by the reaction for tyrosine, and a simultaneous absence, or only traces of acid mucosubstances. Contrary to existing literary reports, an intraplasmatic formation of mucin has been detected in certain thyroid carcinomas and their metastases. The most difficult to diferentiate histochemically from thyroid colloid is the secrete of certain adenocarcinomas of the prostate.

Colloids↗