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Biomedical subjects

F Ferchal

Publications and source records attributed to F Ferchal.

At least 37 records · Page 2Linked to original sources
PubMed1984

Comparison of six methods for detecting human rotavirus in stools.

The following six methods for detecting rotavirus in human faecal samples were compared: electron microscopy, immune electron microscopy, immunofluorescence in cell culture, two enzyme immunoassays (Rotazyme, Enzygnost ) and a latex agglutination test ( Rotalex ). Specimens were collected from 112 children with diarrhoea. The relative sensitivities of the different assays for human rotavirus were as follows: electron microscopy, 84%; immunofluorescence, 86%; Rotalex , 88%; Rotazyme, 89%; immune electron microscopy, 93%; Enzygnost , 98%. According to our findings Enzygnost is the most sensitive method, but Rotalex is more valuable for screening a small number of faecal samples. No false-positive results were observed in the two enzyme immunoassays or in Rotalex .

Child, Preschool↗
PubMed1984

[Parvovirus (serum-parvovirus-like-virus) and Aurillac antigen. A study of 18 cases].

Two cases of parvovirus viraemia were observed in 1972 by screening of blood donors for HBs Ag by counter-electrophoresis. This virus was named "Aurillac" from the town in which the first case originated. In London, a virus found in the same circumstances in 1975, was found identical to the Aurillac Ag. The authors named it "serum parvovirus-like virus" (SPLV) or parvovirus B 19. Since 1972, 16 additional cases have been observed in Paris. Among these 18 cases, 11 were blood donors, 4 were hospital staff and 3 were patients. Second serum samples were available for 13 of the 18 cases. Seroconversion has been detected in all except one, a patient with lymphosarcoma. Antibody could be observed within few days. Anti-Aurillac antibody was found by CEP in 25,3% of blood donors. Examination of antigen positive sera by immune electron microscopy using a late serum sample showed aggregates of spherical particles measuring 20 nm in diameter. The antigen banded in caesium chloride at a density of 1.38. Ten of the 15 healthy adults could be interviewed: 7 appeared symptomless, 1 had a fever up to 39 degrees C for one day, 2 had erythema (as well as 3 subjects who had been in contact with these 2 persons). These cases are discussed and compared to the British literature showing that this virus could provoke the fifth disease or erythema infectiosum and aplastic crisis in chronic hemolytic syndromes. This virus could contaminate clotting factors.

Adolescent↗
PubMed1984

[Immunity related to cytomegalovirus after allogenic bone marrow transplantation: role of donor immunity to cytomegalovirus in the incidence of cytomegalovirus infection in recipients].

Cytomegalovirus infections are severe and frequent after BMT. This study included 34 bone marrow transplant recipients (23 aplastic anaemias and 11 leukaemias), their marrow donors and 125 related or non related normal controls. Assays were performed before transplantation and every 30 days between D 0 and D 90, and then every six months. They included detection of CMV induced lymphocyte proliferation in vitro, CMV antibody determinations by complement fixation and reverse haemagglutination, viraemia and/or viruria. Similarly, cellular immunity to mitogens and to other specific antigens was evaluated. During the period of study, 22 patients developed CMV infection. The diagnostic was confirmed by virus isolation from the 12th to the 96th day after the graft. Development of positive CMV proliferation test occurred from the 9th to the 84th day after virus isolation (30 to 120th day after the graft). In one case, the CMV infection was only proved by the lymphocyte proliferation to CMV in vitro and only 60 days later by viruria and 105 days later by detection of CMV antibodies. For the other 12 patients (7 aplasies and 5 leukaemias) and 10 of their bone marrow donors, no CMV infection was proved, before or after transplant, by any of the assays performed. By selecting a donor without previous CMV infection, we hope to reduce the incidence of CM infection in recipients.

Anemia, Aplastic↗
PubMed1983

[Herpesvirus infections during treatment inducing remission of acute leukemia].

Herpes virus hominis infection was observed in 28% (diagnosis ascertained on viral examination) to 33% (clinical suspicion) of 36 adult patients prospectively studied during remission induction chemotherapy for acute leukaemia. Symptoms are non specific so that diagnosis relies upon viral isolation. All infected patients but one survived the viral infection and the prognosis of the underlying acute leukaemia was unaffected.

Acute Disease↗
PubMed1983

[Herpes virus infections during a remission induction treatment in acute leukemias].

Herpes virus hominis infection was observed in 28% (diagnosis assessed on viral examination) to 33% (clinical suspicion) of 36 adult patients prospectively studied during remission induction chemotherapy for acute leukaemia. Symptoms are non specific so that diagnosis relies upon viral isolation. All infected patients but one survived the viral infection and the prognosis of the underlying acute leukaemia was unaffected.

Acute Disease↗
PubMed1983

[Fatal herpetic hepatitis in an apparently healthy adult].

The authors report the case of a 46-year old patient who died from fulminant herpetic hepatitis. No cause of immuno-depression was documented in this patient. No skin or mucosal herpetic lesion was found except a questionable urethritis. Herpes virus was demonstrated in the hepatocytes by electron microscopy and isolated from the serum. It was identified as herpes virus hominis type II. The low titer of circulating antibodies did not permit the distinction between herpetic primo-infection and reactivation. The features of the hepatic injury are discussed and compared with previous reports. An active diagnostic approach of herpetic hepatitis is considered.

Acute Disease↗
PubMed1981

[Search of a cytomegalovirus infection during pseudo-membranous colitis. 6 cases (author's transl)].

Six patients with pseudomembranous colitis unrelated to lincomycin or clindamycin treatment were investigated for signs of localised or diffuse cytomegalovirus (CMV) infection. Before the onset of colitis 3 patients had receive prednisone, associated in 2 of them with immunosuppressive drugs. Testing for CMV included rectal mucosa biopsies, culture of blood leucocytes with human embryo diploid fibroblasts in continuous layer and titration of complement-deviating anti-CMV antibodies. Cytomegalic cells with nuclear inclusion bodies were found in the rectal mucosa of 5 patients, 4 of whom also had foci of CMV-infected cells in leucocyte-fibroblast cultures, indicative of viraemia. The fifth patient was not tested for viraemia but developed very high anti-CMV antibodies titers at a later stage. These results show that pseudomembranous colitis that are not due to antibiotics are frequently associated with localised or diffuse CMV infection. Viral invasion of the colon might be encouraged by a state of immunodeficiency.

Adult↗
PubMed1980

[Presence of enteric viruses in non-diarrheic canine stools].

Rota-, corona- and parvovirus particles have been visualized by direct electron microscopy in canine stools collected at random in Paris streets. A possible involvement of these viruses in gastroenteric diseases is discussed in the light of these findings.

Animals↗
PubMed1980

Electron microscopy detection and characterization of viral particles in dog stools.

A number of apparently normal dog stool samples, randomly collected on the sidewalks of Paris were examined by Electron Microscopy. The study revealed the presence of viral particles in 27 cases. Morphological criteria lead to the characterization of rotavirus in 2 specimen, coronavirus in 7 and parvovirus in 5. Rotavirus particles appeared always alone while coronavirus and parvovirus particles were present together in 5 cases. Similar particles have been implicated in animal and human gastroenteritis. The importance of their presence in canine dejections is discussed in view of pollution by dog stools of urban and suburban areas.

Animals↗
PubMed1980

[Determination of IgM antibodies by complement-fixation after serum fractionation by sucrose density gradient in children and adults with evidence of Mycoplasma pneumoniae infection (author's transl)].

Sera from 45 patients with serologic evidence of Mycoplasma pneumoniae infection were fractionated by sucrose gradient centrifugation. The distribution of complement-fixing (CF) antibodies for Mycoplasma pneumoniae within the 19S and the 7S immunoglobulins was determined all patients below 20 years of age and 44% of older patients had 19S specific antibodies. A predominance of 19S relative to 7S CF antibodies was observed in 40% of cases below 20 years and in 18% of cases above 20 years. The meaning of these data is discussed.

Adolescent↗
PubMed1979

[Comparative study of anti EBV antibodies in non-Hodgkin's lymphomams and angio-immunoblastic lymphadenopathies (author's transl)].

Antibody titers to Epstein-Barr virus (EBV)--related antigens (viral capsid antigen : VCA; early antigen : EA; and EBV associated nuclear antigen : EBNA) were determined in the sera of 86 patients and 150 matched control subjects. The patients belonged to four histological groups : diffuse and nodular non-hodgkin's lymphomas angio-immunoblastic lymphadenopathies and apparented syndroms. The incidence of antibodies to other herpes-viruses (cytomégalovirus, herpes simplex virus, and varicella zoster virus) was compared. There was a significantly higher incidence of anti VCA and anti EA titers in some patients, not associated with an increase in titres of antibodies to other herpes viruses.

Adolescent↗
PubMed1979

[A method for the preparation of a Chlamydiae group-specific antigen on hela-229 cells infected with a strain of "Chlamydia trachomatis" for use in the complement fixation test (author's transl)].

A simple method for the preparation of a potent group-specific antigen on HeLa-229 cells infected with MRC-1 (LB) (TRIC/GB/MRC-1 Gf) strain of Chlamydia trachomatis is outlined. HeLa-229 cells are infected (MRC-1 strain, 102 inclusion-forming units per cell) with centrifugation at 4,000 g for 1 h in flat-bottomed vials. The cells are removed by brief trypsinization with 0.25% trypsin and put into 75 cm2 culture flasks (12 x 106 cells by flask) in BHK-21 medium supplemented with foetal bovine serum. The flasks are incubated for 5 days at 37 degrees C. The destroyed cell monolayer and the supernatant are centrifuged at 100,000 g for 1 h. The pellet is collected and resuspended in PBS and subjected to ultrasonic vibration for 30 min. This antigen may be used for detection of complement-fixing antibodies in lymphogranuloma venereum and ornithosis.

Antigens, Bacterial↗