Biomedical subjects

F Fetissof

Publications and source records attributed to F Fetissof.

At least 55 records · Page 3Linked to original sources

PubMed1990

[Cytokeratins and exocervical malpighian epithelium. Particular distribution of several cytokeratins].

The purpose of this study was to determine tissue distribution of certain cytokeratins along the ectocervical lining. A series of 20 cases was investigated by immunocytochemical labelling with specific antibodies against cytokeratins 19, 18, 8, 7, 10. Monoclonal antibodies were used on frozen sections. Distribution of keratin or alteration in expression was correlated with morphologic changes. All specimens were lined by a normal stratified squamous epithelium which was devoid of argyrophilic cells. Low molecular weight cytokeratins 8, 18, 19 (normally expressed in simple epithelia) were consistently and exclusively expressed through out the basal cell layer of the ectocervical lining. Some other particular immunostainings have been noted. A peculiar staining pattern was observed around some stromal papillae. Development of papillae was accompanied by the expression of cytokeratin 10 (which is commonly detected in skin), and by a reduction in the level of expression of simple epithelial cytokeratins. In addition, in the middle zone, anticytokeratin 10 revealed a mosaic of positive and negative cells even in light microscopically non keratinizing squamous epithelium, with differences of expression from case to case.

Cervix Uteri↗

PubMed1990

[Endocrine cells and Malpighian epithelium. Merkel-type cells? Immunohistochemical and ultrastructural study].

This study was devoted to a peculiar population of argyrophilic cells present among various stratified squamous epithelia. The purpose of the study was to determine the distribution and the nature of these argyrophilic cells, and to reappraise their degree of analogy with the Merkel cells of epidermis. Examples of squamous epithelia from skin, exocervix, anal canal and dermoid cyst of ovary were investigated using histochemical, immunohistochemical and ultrastructural techniques. Melanin-containing cells as well as peculiar argyrophilic cells were revealed in each series of specimens. These peculiar argyrophilic cells expressed EMA, chromogranin A and NCL-5D3 (cytokeratins 19, 18, 8) immunoreactivities. By contrast, they were serotonin negative. An intense staining for EMA was observed. These cells were obviously endocrine cells. Ultrastructural studies confirmed the presence of endocrine cells within the anal canal and the exocervix; moreover, in anal canal, some of these cells appeared to have contacts with a nerve terminal. Through the epidermis, these cells can only correspond to Merkel cells, because of their chromogranin positivity. Throughout other squamous epithelia, this population of endocrine cells (serotonin negative) bear some analogies with Merkel cells of epidermis. Their distribution, morphology, EMA immunoreactivity and ultrastructural appearance were reminiscent of those of the Merkel cells. They could be named Merkel-type cells. Additional studies, using other characteristic or specific markers of Merkel cells, are clearly required to determine the exact degree of analogy between these types of cells. Merkel-type cells could be largely distributed through stratified squamous epithelia from various tissues. They can be easily visualized by their strong EMA immunoreactivity and must be distinguished from Paget cells.

Endocrine Glands↗

PubMed1990

[Wilms' tumors in adults].

Two cases of Wilms' tumors in adults are reported according to the criteria defined by Kilton. The study of the literature allows defining the pathogenetic hypothesis for these embryonic tumors, as well as their peculiarities in comparison with those occurring in childhood. The prognosis seems to be improved by an aggressive treatment. A schedule of treatment including initial surgery, radiation therapy and chemotherapy varying according to the tumoral group (NWTS 2 staging) is proposed.

Adult↗

PubMed1990

[Epithelial cancer of the ovary in adults: prognostic and therapeutic study of 95 cases].

The authors report 95 cases of ovarian carcinoma treated between 1st January 1975 and December 1986 in the department of gynecology and radiotherapy of hospital Bretonneau in Tours. The overall actuarial 5-year survival was 37.5%, for stage I 100%, stage II 51%, stage III 36%, stage IV 5%, grade I 88%, grade II 51%, grade III 18%. The authors stress the prognostic importance of histologic grade and the bad prognosis of small cell ovarian carcinoma with hypercalcemia. The authors propose a therapeutic attitude based on the results and a review of the literature.

Actuarial Analysis↗

PubMed1990

[Primary non-Hodgkin's high grade malignant lymphoma of the digestive system. A prospective study of the combination of surgery-chemotherapy-radiotherapy].

We report a prospective study concerning the association of surgery-chemotherapy and radiotherapy in the treatment of primary high grade digestive non-Hodgkin's lymphomas in 19 patients. The analysis of 11 stages IE, 5 stages II1E and 3 stages II2E allowed us to evaluate the efficacy and the tolerance of this triple therapeutic association. Fifteen patients are alive and well with a median follow-up of 54 months. One of these patients relapsed, but after autologous bone marrow transplantation is in second unmaintained complete remission. Four patients died of intercurrent aetiology although one death was related to treatment morbidity. Our results and the analysis of literature data lead us to recommend the triple association in the treatment of stage II2E high grade primary digestive lymphomas (PDL) and for PDL without complete resection. However, surgery and chemotherapy appear to be sufficient in the treatment of stages IE and II1E with complete resection.

Adult↗

PubMed1990

[Pure primary retroperitoneal seminoma in a woman. Apropos of a case].

The authors report a case of a stage IV retroperitoneal seminoma in a patient of 43 years of age diagnosed after histological examination of a biopsy specimen from the left sub clavicular lymph mode showed metastatic seminomatosis. After complete assessment including and abdominal and pelvic ultrasound, a CT scan of the abdomen and pelvis, an intravenous urogram, a lymphogram and serial estimations of tumour markers (alpha feta protein and beta HCG) a laparotomy was carried out. This made it possible to take a retroperitoneal biopsy as well as to carry out a total hysterectomy without conservation of the ovaries. From all this we concluded that we were dealing with a straightforward very large retroperitoneal seminoma. The treatment was continued with large doses of chemotherapy using Vinblastin, Cisplatin and Bleomycin (V.C.B.). She received 5 treatments of this and then had radiotherapy. The authors report this first case described in the literature (because they found no other in women) and what made them decide on the therapy.

Adult↗

PubMed1989

Endocrine cells in human Bartholin's glands. An immunohistochemical and ultrastructural analysis.

Endocrine cells were investigated in human Bartholin's glands by use of histochemical, immunohistochemical and ultrastructural methods. Endocrine cells represent normal constituents of these glands, being mainly distributed throughout the transitional epithelium of the major excretory duct; however, single elements are dispersed among the acinar lobules. Serotonin-, calcitonin-, katacalcin-, bombesin- and alpha-hCG-immunoreactive cells were recognized, with serotonin-immunoreactive cells predominating. Co-expression of calcitonin, katacalcin or alpha-hCG with serotonin was observed in single endocrine cells. At the ultrastructural level, these cells are richly granulated and show typical neuroendocrine features. Bartholin's glands display an endocrine profile quite similar to that of other cloacal-derived tissues.

Antibodies↗

PubMed1989

[Malignant mesodermal mixed tumor of the ovary with neuro-ectodermic differentiation].

A striking ovarian tumour was observed in a post-menopausal, 54-year-old woman. Most of the tumour shows histologic features of a malignant mesodermal (müllerian) mixed tumour. However, this tumour harbours a peculiar component suggesting neuroectodermal neoplasia. Histochemical and immunohistochemical studies demonstrate focal pheochromocytomatous (or paraganglionic) and ganglionic differentiations. Several types of cells could be identified: ganglion cells (NSE +, neurofilaments +), pheochromocytes or paraganglion cells (chromogranin +), Schwann cells (S 100 protein +), and neuroblastomatous elements (NSE +). This neoplastic component could be compared with compound tumours such as pheochromocytomaganglioneuroblastoma or gangliocytic paraganglioma. This type of tumour has not been reported in ovary, notably in association with a mesodermal mixed tumour. Such observation prompts us to consider tumours of somatic origin containing germ cell-type elements, with a review of the literature.

Female↗

PubMed1989

[Prostatic type papillary tumor of the urethra with endocrine cells. Immunohistochemical study].

A peculiar variety of prostatic neoplasm harbouring a constellation of endocrine cells is reported. The tumor had a papillary growth projecting into the lumen of the prostatic urethra. The histological appearances were somewhat unusual. One fragment showed a typical feature of a prostatic-type polyp. However, much of the tissue were covered by a dysplastic epithelium. Adenomatous transformation of prostate-type polyp or "endometrial" adenocarcinoma could be considered.

APUD Cells↗

PubMed1988

Endocrine cells in the excurrent duct system of the testis. An immunohistochemical analysis.

A systematic search for endocrine cells in the excurrent duct system of the testis was carried out by means of histochemical and immunohistochemical techniques. A panel of antibodies against amine and polypeptide hormones was used. 80 specimens comprising representative areas of rete testis, ductuli efferentes, ductus epididymis and 30 examples of ductus deferens were investigated. Cells immunoreactive for serotonin were detected in four out of 110 specimens. They were invariably in normal-appearing ductuli efferentes. A salient feature was their rarity and focal distribution. We failed to detect any endocrine cells in other segments of the excurrent duct system and notably not among epididymal epithelium. It seems of interest that serotonin cells are specifically distributed throughout remnants of excretory mesonephric tubules in both males and females.

Endocrine Glands↗

PubMed1988

[Chorionic gonadotrophic hormone and transitional cell carcinoma of the bladder. Immunohistochemical characterization of HCG and its alpha and beta subunits].

Investigation of cellular localization of hCG and alpha- and beta- subunits in 17 transitional cell carcinomas of urinary bladder was performed using immunohistochemical techniques. Serial sections were tested with monoclonal antibodies to hCG, free beta hCG and free alpha hCG. Transitional cell carcinomas producing hCG and/or beta hCG are easily found (6/17 cases). None of these tumors correspond morphologically to a choriocarcinoma. However, it must be mentioned that neoplastic areas exhibit some features reminiscent of a choriocarcinomatous structure. Notably, positive cells can be disposed at the periphery of neoplastic nests, mimicking the arrangement of syncytiotrophoblast. Positive neoplastic cells express hCG and/or beta hCG; alpha hCG immuno-reactivity is quite rare. The relative distribution of hCG/beta hCG is excessively variable from case to case. In contrast, normal transitional epithelium contains endocrine cells which only express the alpha-subunit of hCG.

Carcinoma, Transitional Cell↗

PubMed1988

[Endocrine cells in Walthard's cell rests].

Walthard cell nests have been systematically examined for the presence of some endocrine cell types using histochemical and immunohistochemical techniques. Serotonin immunoreactive cells were the only endocrine cells demonstrated in this site. These cells were immunocharacterized using a peroxidase-antiperoxidase technique. This finding reinforces the homology with transitional epithelium (urothelium) of urinary tract and Brenner tumors. In all these structures, the abundance of endocrine cells seems to be related to the intensity of the proliferative process affecting the transitional epithelium.

Endocrine Glands↗

PubMed1987

Endocrine cells in ectocervical epithelium. An immunohistochemical and ultrastructural analysis.

A systematic study of endocrine cells in the ectocervix was carried out using histochemical, immunohistochemical and ultrastructural techniques. Serotonin and calcitonin immunoreactive cells were demonstrated in this site. Serotonin and calcitonin immunoreactivities were coexpressed in the same endocrine cell. These distinctive cells were encountered in two main morphological varieties of ectocervical epithelium. Normal-appearing stratified squamous epithelium contained only very rare serotonin and calcitonin cells. In contrast, endocrine cells were fairly abundant in a specific epithelium termed "transitional-like". This type of epithelium was not only confined to the transformation zone but could also extend onto the portio as far as the vaginal cut margin. In some cases, transitional-like epithelium bore morphological resemblance to urothelium. In other cases, it could be regarded as basal cell hyperplasia or immature squamous metaplasia. Of interest, serotonin and calcitonin cells have been well-documented as normal inhabitants of some other non-squamous epithelia, such as urothelium or pseudostratified columnar epithelium. Therefore, it is suggested that certain ectocervical epithelia show some similarities to urothelium, in respect of their morphological appearance and endocrine profile. Further investigations using more objective and specific markers of urothelial cells are needed to assess the exact degree of homology connecting all these types of epithelium.

Calcitonin↗

PubMed1987

Glycoprotein hormone alpha-chain-immunoreactive endocrine cells in prostate and cloacal-derived tissues.

All cloacal-derived tissues contain a similar endocrine cell population. Human chorionic gonadotropin (hCG)-like immunoreactivity has been reported in some prostatic endocrine cells. On the other hand, immunoreactivity to alpha-hCG is well known to occur in a variety of extraprostatic endocrine elements. Most cloacal-derived tissues were tested with a panel of monoclonal antibodies against hCG and subunits. The previously described hCG-like positivity was found to be exclusively related to the free alpha-subunit of hCG. Moreover, alpha-chain immunoreactivity was more broadly distributed in most cloacal-derived tissues, including those from the anal canal. In the anal canal, alpha-chain was an excellent marker of the anal transitional zone. alpha-Chain-immunoreactive endocrine cells were normal and nonneoplastic constituents of these tissues; they corresponded to a subpopulation of serotonin cells. In addition, certain prostatic argyrophil cell carcinomas might be exclusively alpha-subunit producing.

Calcitonin↗

PubMed1987

[Malignant mixed mesodermal tumor with endocrine cells of the corpus uteri].

A case of uterine malignant mixed mesodermal tumor harboring endocrine cells is reported. Endocrine cells were immunocharacterized as serotonin, somatostatin and pancreatic polypeptide cells. This variety of mixed müllerian tumor is added to other endocrine cells-containing neoplasms of the uterus.

Adenocarcinoma↗

PubMed1987

[Cutaneous angiosarcoma of the breast following surgery and irradiation of breast adenocarcinoma].

We are reporting a localised skin angiosarcoma of the breast. As far as we can tell this is only the fourth time this condition has been published. The tumour appeared 6 years after a conservative lumpectomy with removal of lymphatics and follow-up radiotherapy had been carried out for an adenocarcinoma of the breast. This tumour was characterised by the presence of several round blue-coloured nodules in the skin with some inflammation surrounding them. The glandular tissue underneath was normal, as was the opposite breast, and there was no spread. The patient was pyrexial and a syndrome of inflammation persisted until the tumour had been removed by a simple mastectomy. We have discussed the clinical and histological aspects of this case and compared them with those found in the literature. Differential diagnosis with in particular Stewart-Treves syndrome has been emphasized. The treatment consists in wide excision and sometimes followed by radiotherapy or chemotherapy. The prognosis is very poor, survival never having been more than 22 months. Finally the role of irradiation in the causation of this tumour has been discussed. (Our patient is alive after 2 years without any sign of a recurrence).

Adenocarcinoma↗

PubMed1986

Calcitonin immunoreactive cells in prostate gland and cloacal derived tissues.

Calcitonin- and serotonin-storing cells have been immunocharacterized in prostate gland, urethra, urinary bladder and anal canal. In addition, a few hCG and somatostatin immunoreactive cells have been detected in prostate gland. All these cells were dispersed throughout the epithelial lining. In the anal canal, calcitonin cells were exclusively confined to the anal ducts and anal transitional zone epithelium. Calcitonin and serotonin cells were seen in some examples of prostatic adenocarcinoma. Combined techniques most often showed coexistence of calcitonin and serotonin immunoreactivities in the same endocrine cell. hCG immunoreactive cells corresponded to a subpopulation of serotonin-, calcitonin-storing cells. Calcitonin and serotonin cells were present in most organs which originated from the cloaca. In this territory, this distinctive endocrine pattern could be regarded as an excellent marker of cloacal derived tissues. These tissues constitute an additional site for extrathyroid C-cells. It is likely that calcitonin cells are a component of some prostatic adenocarcinomas.

Adenocarcinoma↗

PubMed1986

Endocrine cells in renal pelvis and ureter, an immunohistochemical analysis.

A systematic detection of endocrine cells in the renal pelvis and ureter was carried out, using Grimelius stain and immunohistochemical techniques. Ninety specimens of pelvic and ureteral mucosae were investigated. Throughout the pelvic urothelium, endocrine cells were very uncommon, patchily distributed, and serotonin-storing. They have been disclosed in only two cases among normal-appearing transitional epithelium. Whether cells so scanty are normal and permanent inhabitants of this territory is debatable. The material examined did not provide opportunity to demonstrate endocrine cells throughout the normal ureter. Surprisingly, one case of supernumerary ureter with an ectopic distal orifice into the urethra harbored abundant serotonin cells. This remarkable endocrine profile, which departed appreciably from that of normal ureter, showed a close similarity with that of the urethra. This raises the question whether such endocrine differentiation might reflect, for this ectopic ureteric bud, urogenital sinus origin rather than wolffian origin.

Fluorescent Antibody Technique↗