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Biomedical subjects

F Franke

Publications and source records attributed to F Franke.

At least 55 records · Page 3Linked to original sources
PubMed1978

Improvements of the electrophoretic mobility test to measure lymphocyte sensitization.

The Electrophoretic Mobility (EM)-test was performed in 316 children to examine their lymphocyte sensitization to a common antigen by measuring the mobility of special indicator cells. The results indicate that this test can serve as an additional help in differential diagnosis of malignant and non-malignant diseases. Measurements in the cytopherometer were facilitated by introducing several technical modifications: 1) The original method of timing with a stopwatch was replaced by two electric watches electrically combined with the cytopherometer. Thus individual faults were reduced. The next improvement of timing at the cytopherometer consisted in the construction of an electronic controller and stopwatch in combination with a minicomputer, data print out, and tape recorder in order to get more reliable results. 2) Further automatic recording of the data is time-sparing and useful for correct evaluation of the EM-test. That can be done now directly on-line in a calculator with a program developed in our laboratory. 3) By using a television monitor for the observation of the indicator particles an additional simplification is possible.

Antigens↗
PubMed1977

[Revascularisation after lower-leg arterial block. Report of 115 femoro-crural reconstructions (author's transl)].

Revascularisation after arterial block in the lower leg is a low-risk procedure which is definitely superior to lumbar sympathectomy in stage III and IV. In 110 patients 115 femorocrural reconstructions were performed in almost 9 years. Four weeks after the operation the revascularisation was open and showed free flow in 93.3%. In 64.4% an open revascularisation with largely painless walking ability was found 2-9 years post-operatively.

Adult↗
PubMed1977

[Vascular reconstruction in arterial occlusion of the lower leg (author's transl)].

In 84% of 115 femoro-crural arterial occlusions stage III and IV was present. All operations to revascularisation, including second operations could be performed without lethality. On leaving hospital in 93% the bypass was patent. Two to nine years after the operation in 64% of the venous grafts were good. Only eleven patients have had to subject amputations because of bypass occlusion since reconstruction. Our results prove that the femoro-crural reconstruction in arterial occlusion of the lower leg is superior to lumbar sympathectomy and the conservative treatment.

Adult↗
PubMed1976

The binding of ethyl carbamate to DNA of mouse liver in vivo: the nature of the bound molecule and the site of binding.

Ethyl carbamate, labelled at C1 with 14C, bound in vivo to liver DNA of intact and partially hepatectomised mice. Isotope (18O) enrichment was not detected in the oxygen of liver DNA of mice injected with [18O] ethyl carbamate, C2H5--18O--CO--NH2. This suggests that it was the ethyl group and not the ethoxy group which bound to DNA. Chromatographic analysis of acid hydrolysates of liver DNA from mice treated with [1-14C] ethyl carbamate provided no evidence of alkylation or other form of binding to purine or pyrimidine bases. On relatively mild acid hydrolysis the alkyl group remained bound to the "apurinic acid" fraction, while more vigorous hydrolysis lead progressively first to its separation as highly ionisable hydrophilic non-volatile compounds and then to its loss as a volatile compound. DNAase I followed by phosphodiesterase hydrolysis also split off the 14C-containing group as a volatile compound. The volatile compound was identified as ethanol. These results suggest that the alkyl group was bound as an ester to a phosphate group in the DNA chain. Results with DNA from partially hepatectomised mice did not differ from those with DNA from intact mice.

Animals↗
PubMed1975

[Arterielle occlusion in two leg amputees ("phantom claudication" due to pelvic-artery stenosis) (author's transl)].

Signs of intermittent claudication in the phantom leg developed in two patients 18 and 27 years, respectively, after mid-thigh amputation. Symptomatic treatment of the phantom pain was without success. Clinical, oscillographic and angiographic studies demonstrated atherosclerotic arterial stenoses of the iliac arteries. After successful vascular reconstruction (iliac-femoral and aortic-femoral bypass, respectively) on the side of amputation both patients are without pain or other symptoms and can again walk with restriction.

Angiography↗