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Biomedical subjects

F Fu

Publications and source records attributed to F Fu.

At least 37 records · Page 2Linked to original sources
PubMed1996

[Sixty years of study on ancient medical history].

The paper includes ten parts, viz. origin of medicine, division of historical periods in Chinese medicine, medical institution, history of preventive medicine, history of medicl disciplines and diseases, history of exchanges among China and foreign countries, history of medical education, history of minority medicine, studies on medical figures, works on Chinese medical history. It summarizes the brilliant achievements in the study of ancient Chinese medical history.

China↗
PubMed1995

Growth of donor-derived dendritic cells from the bone marrow of murine liver allograft recipients in response to granulocyte/macrophage colony-stimulating factor.

Allografts of the liver, which has a comparatively heavy leukocyte content compared with other vascularized organs, are accepted permanently across major histocompatibility complex barriers in many murine strain combinations without immunosuppressive therapy. It has been postulated that this inherent tolerogenicity of the liver may be a consequence of the migration and perpetuation within host lymphoid tissues of potentially tolerogenic donor-derived ("chimeric") leukocytes, in particular, the precursors of chimeric dendritic cells (DC). In this study, we have used granulocyte/macrophage colony-stimulating factor to induce the propagation of progenitors that give rise to DC (CD45+, CD11c+, 33D1+, nonlymphoid dendritic cell 145+, major histocompatibility complex class II+, B7-1+) in liquid cultures of murine bone marrow cells. Using this technique, together with immunocytochemical and molecular methods, we show that, in addition to cells expressing female host (C3H) phenotype (H-2Kk+; I-E+; Y chromosome-), a minor population of male donor (B10)-derived cells (H-2Kb+; I-A+; Y chromosome+) can also be grown in 10-d DC cultures from the bone marrow of liver allograft recipients 14 d after transplant. Highly purified nonlymphoid dendritic cell 145+ DC sorted from these bone marrow-derived cell cultures were shown to comprise approximately 1-10% cells of donor origin (Y chromosome+) by polymerase chain reaction analysis. In addition, sorted DC stimulated naive, recipient strain T lymphocytes in primary mixed leukocyte cultures. Evidence was also obtained for the growth of donor-derived cells from the spleen but not the thymus. In contrast, donor cells could not be propagated from the bone marrow or other lymphoid tissues of nonimmunosuppressed C3H mice rejecting cardiac allografts from the same donor strain (B10). These findings provide a basis for the establishment and perpetuation of cell chimerism after organ transplantation.

Animals↗
PubMed1995

Cytokine mRNA profiles in mouse orthotopic liver transplantation. Graft rejection is associated with augmented TH1 function.

Although mouse liver allografts are spontaneously accepted without immunosuppression in many strain combinations, rejection can be induced by presensitization with a donor skin graft two weeks prior to transplantation. In this study, the semiquantitative reverse transcription polymerase chain reaction (RTPCR) was used to assess the involvement of T helper (TH) cell subsets in liver allograft acceptance by determining cytokine mRNA in the graft and spleen of recipients with (A) spontaneously accepting allografts (B) rejecting liver allografts after previous skin sensitization, and (C) syngeneic controls. Spontaneously accepted liver allografts showed upregulation of TH1 (IL-2, IFN-gamma) and TH2 (IL-4, IL-10) intragraft cytokine mRNA, which peaked at day 6 and tapered off thereafter, when compared with levels in syngeneic grafts, but both IFN-gamma and IL-10 mRNA persisted up to day 30. This cytokine mRNA profile correlated with the transient intragraft inflammation associated with spontaneously resolving rejection. Presensitized recipients that rejected their grafts revealed marked upregulation of TH1 (IL-2 and IFN-gamma) and TH2 (IL-4, IL-6) intragraft cytokine mRNAs compared with spontaneously accepting recipients, although IL-10 mRNA levels showed no differences between the two groups. The most striking difference was seen in IFN-gamma levels, which correlated well with the preferential deposition of IgG2a antibody isotype in the rejecting compared with the spontaneously accepting liver allograft recipients. These results suggested an association between liver allograft rejection and enhanced TH1 cytokine immune response. The ability to reject liver allografts by the adoptive transfer of splenocytes, but not serum, from a sensitized mouse ruled out preformed antibodies alone as a cause of rejection. However, spleen cytokine mRNA profiles showed no differences or trends in TH1 or TH2 expression in spontaneously accepting versus rejecting recipients, which suggested that the spleen is not a major site of alloreactive immune expansion. These data suggest that spontaneous acceptance of mouse liver allografts is associated with an insufficient intragraft TH1 cytokine response, the cause of which is currently under investigation.

Animals↗
PubMed1995

Validity of conventional anthropometric techniques for predicting body composition in healthy Chinese adults.

There is little information on the application of generalized prediction equations to ethnic groups other than Europeans and groups of European descent. The purpose of this study was to crossvalidate conventional equations on a group of 56 Chinese adults. Body density was assessed by underwater weighing and also predicted by equations which use a combination of selected skinfolds-biceps, triceps, pectoral, subscapular, abdominal, suprailiac, thigh and calf. There were significant correlations (P < 0.01) between the various methods of predicting percentage fat. However, analysis of variance revealed significant differences (P < 0.01) between mean values. In the men, the Jackson and Pollock equation underestimated, and the Durnin and Womersley equation overestimated, the percentage fat predicted by underwater weighing. The best predictor site in this group was the medial calf skinfold (r = 0.81), which is not included in either equation. In the women, the best predictor sites were the triceps, suprailiac and thigh. As these sites are also used in the Jackson et al. equation, it is not surprising that there was no difference between the prediction of percentage fat by this equation and underwent weight. It is concluded that the Durnin and Womersley and Jackson and Pollock equations tend to overestimate and underestimate, respectively, the percentage fat in Chinese men. Alternative equations which use the calf skinfold may be more appropriate for this ethnic group. In Chinese women, there appears to be good agreement between Jackson and Pollock and hydrodensitometric estimations of percentage fat.

Adult↗
PubMed1995

Presensitization by skin grafting from major histocompatibility complex class I or major histocompatibility complex class II deficient mice identifies class I antigens as inducers of allosensitization.

Livers but not hearts are accepted spontaneously without immunosuppression when transplanted from B10 (KbAbEbDb) to C3H (KkAkEkDk) mice. Both organs however, undergo accelerated rejection in C3H recipients presensitized with B10 skin grafts. In this study, we have investigated further the role of functional cell-surface major histocompatibility complex (MHC class I or class II molecules in allosensitization. Skin from transgenic MHC class I (b2mmlUncbcr; AbEb) or class II (C2DTM, KbDb) gene 'knockout' mice was grafted onto naive recipients 2-3 weeks prior to whole organ transplantation. When C3H hosts were presensitized with skin from C2DTM (class II deficient) mice, they promptly rejected (within 4 days) subsequently transplanted B10 liver or heart allografts. In contrast, presensitization with skin from b2m (beta 2-m mutant; class I deficient) mice did not significantly affect the survival of either organ graft. Maximal sensitization was established by day 14 after skin grafting and persisted for at least 12 weeks. Splenocytes obtained from C3H mice sensitized with skin from B10, B6 (KbAbEbDb), or C2DTM but not from b2m mice exhibited an H-2b-specific cytolytic response when tested in cell-mediated lymphocytotoxicity assays. Sera from C3H mice sensitized with B10 or b2m skin contained high titres of cytotoxic activity specifically against H-2b class I. Taken together, these observations suggest that in the strain combination studied, MHC class I rather than class II molecules play an important role in allosensitization. The results indicate the potential importance of avoiding transplantation of organs into recipients of secondary grafts from donors that share human leucocyte antigen (HLA) class I antigens with the first donor.

Animals↗
PubMed1995

[Acridine mutagen ICR-170 induced developmental suppression of ovary and vitelline gland in female Schistosoma japonicum].

Mice of Kunming strain were infected with Schistosoma japonicum cercariae previously incubated with various concentrations of acridine mutagen ICR-170 for different time durations. At 6 weeks after infection, the mice were autopsied. The results showed that 24 out of 28(85.7%) adult female worms had deformed or lacked ovaries and vitelline glands when the cercariae were treated with the agent at a concentration of 10 micrograms/ml and incubated at 30.5 degrees C for 30min. No apparent changes were observed in the male worms inhabiting the mesenteric and portal veins with the females worms in their gynecophoral canals. The mutagenized female schistosomes obtained from the present experiment might be served as another form of attenuated worms for the induction of protective immunity.

Aminoacridines↗
PubMed1994

Split tolerance induced by orthotopic liver transplantation in mice.

Spontaneous orthotopic liver allograft acceptance associated with microchimerism in mice induces tolerance to subsequent skin or heart transplants from the donor but not third-party animals. Despite in vivo hyporesponsiveness, in vitro MLC and CTL assays showed continuing antidonor reactivity. Cells isolated from recipients' spleens and grafted livers, when tested in MLC and CTL assays, were antidonor reactive out to 3 months to the same degree as splenocytes obtained from either naive or presensitized (with skin or heart) mice. Nevertheless, passive transfer of splenocytes or liver lymphocytes from liver tolerant mice, but not naive or sensitized donor strain mice, were able to prolong skin graft survival significantly in naive irradiated recipients. By using a strain combination in which the donor but not the recipient expressed the stimulatory endogenous super-Ag (Mlsf), it was possible to determine whether super-Ag-reactive T cells bearing V beta 5 and V beta 11 were deleted or anergic. Phenotypic analysis of cells isolated from recipients' spleens and grafted livers (up to 90 days after transplant), when compared with naive animals, showed no significant difference in V beta 5 and V beta 11 TCR expression. Additionally, when these isolated spleen cells were tested for antibody-mediated stimulation, both anti-V beta 5 and V beta 11 TCR mAb led to marked proliferation of cells obtained from naive and liver-transplanted recipients, but as expected, proliferation was very low in cells from naive donors. These results suggest that liver transplantation induces donor-specific tolerance in vivo, which may not be reflected in in vitro proliferative and cytotoxicity assays (split tolerance). Furthermore, this tolerance does not seem to be induced by clonal deletion or anergy of minor-lymphocyte-stimulating-antigen-reactive T cells in the recipients.

Animals↗
PubMed1994

Intraarticular contact stresses with simulated ankle malunions.

Peak contact stresses were evaluated in a human cadaver ankle model of ankle fracture malunion. In one set of experiments, changes in peak contact stresses were evaluated for ankles loaded with lateral displacements of the talus and fibula of 1, 2, 3, and 4 mm. In a second set of experiments, peak contact stresses were evaluated after removal of posterior malleolar fragments. Peak contact stresses were concentrated over the anterior medial talar dome in normal specimens, and in specimens with stimulated malunions. There were relatively low stresses beneath the medial malleolus. Peak stresses for ankles without talar displacements ranged from 1.9 MPa to 12.4 MPa (eliminating one disparate specimen). There was no increase in peak stresses with lateral displacements of the talus or with removal of posterior malleolar fragments. The fact that peak stresses with simulated ankle malunions were not elevated suggests that factors other than the magnitude of normal contact stresses are of greater importance in the pathogenesis of posttraumatic arthritis.

Ankle Injuries↗
PubMed1994

Estimation of body composition in Chinese and British men by ultrasonographic assessment of segmental adipose tissue volume.

It has been shown that ultrasonographic measurements can be used to predict body composition in adults. The purpose of this study was to assess the relationship between ultrasonograph and caliper (SKF) measurements of subcutaneous adipose tissue thickness in athletic Caucasian (English, E) and Asian (Chinese, C) men against estimates of body composition determined from hydrodensitometry (HYD). The usefulness of a proposed ultrasonographic method of estimating lean and fat proportions in the upper and lower limbs was also evaluated as a potential method of predicting body composition. Ultrasonography (US) was used to measure adipose and skin thickness at the following sites: biceps, triceps, subscapular, suprailiac, abdominal, pectoral, thigh and calf. Caliper measurements were also made at the above sites. Subcutaneous fat thickness and segmental radius were measured directly from the display screen of the ultrasonic scanner (Aloka 500 SD). By applying the geometry of a cone, the proximal and distal radii of the upper arm and upper leg were used to calculate the proportionate volumes of adipose tissue. The best correlations for US and SKF were obtained at the quadriceps, subscapular and pectoral sites for E (r = 0.96, 0.93 and 0.90, respectively) and at the quadriceps, calf and abdominal sites for C (r = 0.90, 0.81 and 0.75, respectively). The best ultrasonographic predictor of the percentage fat in both groups was the percentage adipose tissue volume in the upper leg (r = 0.83 and 0.79 for C and E, respectively). Stepwise multiple regression analysis indicated that the prediction of percentage fat was improved by the addition of the ultrasonographic abdomen measurement in both groups: Chinese sample: %fat = %fat(leg) (0.491) + US abdomen (0.337) + 0.95 ( R = 0.89, s.e.e. = 1.9%); English sample: %fat = %fat(leg) (0.435) + US abdomen (0.230) - 0.765 ( R = 0.80, s.e.e. = 3.6%). It is concluded that ultrasonographic measurements of subcutaneous adipose tissue and volumetric assessment of percentage adipose tissue in the thigh are useful estimates of body composition in athletic English and Chinese males.

Adipose Tissue↗
PubMed1993

Fat-free mass estimation by bioelectrical impedance and anthropometric techniques in Chinese children.

There is limited information on the accuracy of bioelectrical impedance analysis (BIA) for estimating body composition in children. The purpose of this study was to evaluate BIA measurements for estimating fat mass and fat-free mass in 94 Chinese boys and girls aged 11-17 years. Percent fat (%fatskf) and fat-free mass (FFMskf) were predicted by regression of skinfolds in an equation which is founded on a multicomponent model of body composition in children. Multiple-regression analyses were applied to the data to determine if height2 divided by resistance (resistance index) (RI) could accurately predict FFMskf and %fat. Correlations (R) and predictive accuracy (standard error of the estimate, S.E.E.) for FFMskf for RI alone were 0.94 and 2.7 kg; for RI and body mass this improved to 0.96 and 2.2 kg, and for estimation of %fatskf from RI and body mass these values were 0.78 and 4.7%, respectively. A previously published prediction equation, developed on Caucasian children and which also used RI and body mass, was also cross-validated with the Chinese sample in this study. There was no difference between the predicted values from this equation and FFM and %fat predicted by the skinfold technique. The correlation coefficient for FFM was 0.96 and the S.E.E. was similar to that originally reported for the Caucasian sample. We conclude that BIA is a reliable and acceptably accurate method of estimating anthropometrically determined body composition in Chinese youth.

Adipose Tissue↗