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Biomedical subjects

F Gabl

Publications and source records attributed to F Gabl.

At least 37 records · Page 2Linked to original sources
PubMed1979

[Measuring the activity of the placental isoenzyme of alkaline phosphatase: 3 methods compared (author's transl)].

Three methods were compared in human serum for determining the activity of the placental isoenzyme of alkaline phosphatase: 1. Heat inactivation at 65 degrees C for 10 minutes, 2. Differential inactivation with L-p-Bromotetramisole, and 3. an immunological precipation test. A good comparison between the three methods was found (correlation coefficient between 0.973 and 0.982). For long series or screening determinations for "Regan-isoenzyme" the differential inactivation with L-p-Bromotetramisole is preferred because of the short analysis time and the possibility of simple mechanisation.

Alkaline Phosphatase↗
PubMed1978

[Results of screening for diabetes within the health-care program of vienna (author's transl)].

Within the health-care program of Vienna 1577 volunteers (1203 female, 374 male persons) were screened for diabetes mellitus during 1975/76. Besides measurement of glucose tolerance one hour after the ingestion of 100 g glucose the following parameters were analyzed: cholesterol, triglycerides, uric acid and blood pressure. Male persons had a slightly decreased glucose tolerance; a distinct relationship with age was found in both sexes. After elimination of volunteers in whom pathological results were obtained with respect to other examined parameters, a significantly greater glucose tolerance was found in the remaining "normal" group. There are no parameters which are closely correlated with glucose tolerance although it was possible to reject the null-hypothesis in several cases due to the large sample involved. For statistical reasons (test sensitivity, test specificity, prevalence of disease) single glucose-tolerance measurements do not give enough evidence for diagnosing diabetes mellitus. Depending on the aims of the screening program one has to define the detection limits as well as frequency of retesting.

Adolescent↗
PubMed1978

[Hypophosphatemia during parenteral nutrition of patients with renal insufficiency (author's transl)].

Hypophosphatemia is a much commoner condition than generally recognized from investigations on this subject so far. Hypophosphatemia may cause ill-defined disturbances in the course of illness in patients with renal insufficiency. On the basis of our results we recommend the addition of 5--10 mmol phosphate (155--310 mg phosphorus) per 1000 kcal. of the nutrient solution right from the start of parenteral nutrition in patients with chronic renal insufficiency. The phosphate dosage must be further increased, at least temporarily, in hypophosphatemic patients with acute renal insufficiency. Serum phosphate determination should be obligatory in patients with renal insufficiency at the time when the patient is first seen. It should also be performed at least every second day during the first week of parenteral nutrition. Thereafter, the determination of serum phosphate twice weekly should suffice to control the dosage of phosphate required by the patient.

Acute Kidney Injury↗
PubMed1977

Serum-induced changes in electrophoretic mobility of creatine phosphokinase isoenzymes?

BB isoenzyme of creatine phosphokinase (CK-BB) obtained from human brain-extract changes its electrophoretic mobility after incubation in human serum at 37 degrees C. No change of electrophoretic mobility of CK-BB is observed after incubation in isotonic saline. We have shown by means of immunoprecipitation with specific antibodies that the structure of CK-BB is not changed. These findings are supported by other authors and make the diagnostic value of electrophoretic separation of CK isoenzymes doubtful as after a 3-h incubation CK-BB migrates similarly to CK-BB and consequently may be misinterpreted.

Brain↗
PubMed1977

[A comparison of serum phosphohexose isomerase and sialyl transferase activities in patients with malignant disease].

The diagnostic value of serum sialyl transferase, phosphohexose isomerase and lactate dehydrogenase determinations is discussed in respect to patients with malignant diseases. It was found that 80 % of the investigated cases showed an increase in serum sialyl transferase activity. Phosphohexose isomerase was elevated in 46.6 % and lactate dehydrogenase in 23.3% of the cases. The serum sialyl transferase activity was within the normal range in 13.3% of the patients. It is concluded that although the serum sialyl transferase activity is of great diagnostic significance in cases of suspected malignancy, total reliance cannot be placed on the determination of this sole parameter, but only in conjunction with others.

Breast Neoplasms↗
PubMed1977

[Isoenzymes of creatine kinase in the perinatal period (author's transl)].

The serum of new-born children shows a higher catalytic concentration of creatine kinase than adult serum. These values reach normal adult levels within the first 10 days of life. In addition to the MM-, the MB- and BB-isoenzymes of creatine kinase are also found in the serum of women in labour, in cord-serum, and in the serum of newborn children. These findings should be taken into consideration in the analysis of creatine kinase isoenzymes.

Adult↗
PubMed1976

Critical role of the conversion of the third complement component C3 (beta 1C/beta 1A) for its immunochemical quantitation.

For the immunochemical quantitation of the third component of complement C3 the state of conversion of this protein in the sample and the reference serum has to be guarded. Antisera raised against C3 may show different antibody specificity for the antigenic pattern of the C3 proteins which change during proteolysis by activation and also by ageing. It is shown that anti-C3-sera gave differences from -17% to + 91% comparing fresh and aged aliquots of the same sera directly on the same immunodiffusion plate corresponding to a particular anti-C3-serum. The use of three different monospecific antisera directed against the A determinant of C3 seemed to give results with good correlation in respect to C3 conversion. Glass and polystyrene plastic tubes did not have a significantly different effect on the C3 values in respect to C3 conversion. The in vitro acceleration of C3 conversion by thrombin or an inulin-like polysaccharide did not give consistent results in this context.

Complement C3↗
PubMed1976

Creatine kinase isoenzymes in cerebrospinal fluid in a case of brain damage.

We present a case of a 11/2-year-old boy with toxic enteritis, consecutive consumption coagulopathy, and sever brain damage. During the acute phase we found high activity of the BB isoenzyme of creatine kinase in cerebrospinal fluid, but not in the serum. Isoenzyme MM could also be found in the spinal fluid (37.9% of the total activity). We conclude that analysis for creatine kinase isoenzymes in spinal fluid is of clinical importance.

Brain Injuries↗