[Serum cholesterol. Comparison of an enzymatic determination method with the extraction method on A.A.II].
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Biomedical subjects
Publications and source records attributed to F Gabl.
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1. 3006 adult volunteers (1001 women after an overnight fast of at least 12 hours and 2005 women and men, 6 to 8 hours after a light breakfast) were investigated in regard to serum cholesterol and triglyceride levels, body weight and height. 2. 73.8% of the men and 75.2% of the women were overweight. The degree of overweight increased with increasing age and only decreased in the advanced age groups. 3. Serum triglyceride and cholesterol values increased with increasing age and showed a decrease in the older groups. 4. Serum triglyceride and cholesterol levels increased with increasing weight, also within each single decade. 5. 21.7% of the 1001 fasting women showed values for TG over 200 mg/dl and/or Chol over 300 mg/dl.
The total activity and the isoenzyme fractions of alkaline phosphatase were determined in the serum of 15 patients on intermittent haemodialysis and 32 kidney transplant recipients. The isoenzymes of alkaline phosphatase were separated by polyacrylamide gel disc electrophoresis. It was apparent that the increased activity of serum alkaline phosphatase in these patients was not limited to a distinct isoenzyme fraction. 5 of the 15 dialysis patients and 19 (59%) of the 32 kidney transplant patients showed a bone-type isoenzyme pattern. The possible causes for the presence of a bone-type alkaline phosphatase pattern are discussed. The fractionation of alkaline phosphatase gives a valuable indication as to the organ of origin of the particular fraction and is also of diagnostic value when the total activity of alkaline phosphatase lies within the normal range.
Of 71 patients showing a negative direct antiglobulin (Coombs') test (DAT) before administration of cephalexin (CEX) three developed a positive DAT after therapy with this drug (4 percent). No case of immunohemolytic anemia was observed. The sera of the patients with a positive DAT reacted with cephalothin-coated red cells producing high titers, with penicillin cells normal titers and with CEX cells low titers. Since the red cell eluates of these patients did not give significant reactions with red cells sensitized with CEX, cephalothin and penicillin, a nonimmunological mechanism for the development of the positive DAT in these patients has to be assumed.
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