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F H Borgsteede

Publications and source records attributed to F H Borgsteede.

86 records · Page 5Linked to original sources

Experimental cross-infections with gastrointestinal nematodes of sheep and cattle.

Five parasite-free lambs and five parasite-free calves were infected experimentally with 20,000 third stage gastrointestinal nematode larvae cultured from faeces of sheep from five different farms. In the same way five lambs and five calves were infected with larvae cultured from faeces of calves from five different farms. Host specificity of the nematodes was determined using the following parameters: infection rate; number, size, and stage of the worms; their distribution within the gastrointestinal tract; and the onset, duration, and level of egg production. The conclusions with regard to the different species are: Almost exclusively in cattle Skrjabinagia lyrata Strongly adapted to cattle Ostertagia ostertagi More adapted to cattle than to sheep Cooperia oncophora, C. surnabada, C. punctata, Trichostrongylus longispicularis No special host preference T. axei, O. leptospicularis, S. kolchida More adapted to sheep than to cattle C. curticei, T. vitrinus, T. colubriformis, O. trifurcata, Teladorsagia davtiani, Haemonchus contortus, Strongyloides papillosus Strongly adapted to sheep O. circumcincta, Oesophagostomum venulosum, Almost exclusively in sheep Chabertia ovina.

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The efficacy of oxfendazole administered as a bolus compared with a drench formulation.

In a flock of 40 ewe lambs of the Texel breed the anthelmintic oxfendazole was tested in two different formulations, a 2.265 per cent suspension and a 4 gram bolus containing 151 mg active ingredient. All treatments were based on a dose rate of 5 mg/kg body weight. Faecal examinations and larval differentiations were carried out on the day of treatment and two and seven days later. No differences in efficacy were apparent between the two treated groups. Oxfendazole in either formulation was 100 per cent effective in removing the major strongylids and trichostrongylids. A lower activity was seen against Strongyloides papillosus.

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[The natural pattern of Ostertagia-infections in cattle (author's transl)].

In experiments carried out under field conditions with calves reared free from parasites and kept on pastures contaminated with Ostertagia larvae, the natural pattern of infestation with this parasite in calves was studied. It was shown that calves turned out early (late in April) developed a much higher primary (overwintered) infestation than calves turned out six weeks later on mown pasture. During the grazing season the serum pepsinogen levels were more indicative of the severity of the infestation than was the egg output. There also was a correlation between the serum pepsinogen level and loss of weight. During the housing period following the first grazing season, adult worms were eliminated more rapidly than inhibited fourth-stage larvae, resulting in a worm population consisting of up to 90 per cent EL-4 at the end of the housing period in April.

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[Nematodirus battus definitely established in the Netherlands (author's transl)].

After Nematodirus battus infection had been detected in sheep imported from Britain into the Netherlands in January 1978, Dutch lambs and sheep on seven farms in the Province of Gelderland were also found to be infected with N. battus later in 1978. The possible origin of the infection was investigated. Three flocks had been in contact with British sheep, two farms were trading farms, one farm had purchased its sheep from various sources a few years previously, on another the cause continued to be obscure, recently purchased rams possibly having played a role in this case. Severe infections were not (sor far) observed. It was difficult to differentiate between clinical symptoms of N. battus infection, if any, and those due to coccidiosis which was diagnosed in a number of flocks.

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The activity of albendazole against adult and larval gastrointestinal nematodes in naturally infected calves in the Netherlands.

The activity of albendazole against gastrointestinal helminths in naturally infected calves in the Netherlands was tested. The calves were in their first grazing season and kept in two groups of ten. One of these groups was grazed alternately with sheep. Five out of each group were drenched with albendazole (7.5 mg/kg) on the day they were housed (November 1). Before and 2, 14, and 28 days after treatment individual faecal samples were taken from all calves and larval cultures were made. Ten calves, six treated and four untreated, were killed for post mortem studies 14 days after treatment. The remaining calves were slaughtered 14 days later. The drug was highly effective in reducing the egg output, measured as the number of larvae cultured per gram of faeces. Compared with the untreated calves, the reduction was more than 99% two days after treatment, 100% at 14 days, and 99% after 28 days. It was shown that egg output 28 days after treatment came from worms which had developed from arrested larvae of Ostertagia ostertagi that had survived treatment. Post mortem results showed an efficacy of 100% against adult O. ostertagi, of almost 100% against Trichostrongylus axei, and 100% against adult and larval Cooperia oncophora. Twenty-eight days after treatment, the reduction of arrested early fourth stages of O. ostertagi was 85% in comparison with the untreated calves. Apparently less effect was found against Trichuris ovis at the given dose rate.

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Experimental infections with Cooperia oncophora (Railliet, 1918) in calves. Results of single infections with two graded dose levels of larvae.

Two experiments were carried out in which calves reared parasite-free were infected with a single dose of 3rd-stage larvae of Cooperia oncophora. In the first experiment the calves received 20 000 or 200 000 infective larvae and they were autopsied 28 or 56 days after the infection. In the second experiment the doses were the same but the animals were killed 14, 84 or 140 days after infection. If a dose of 20 000 was given, clinical signs were never observed, while at a dose level of 200 000 the weight gain was less on 56 and 84 days after the infection compared with the low-infected groups or the control animals. After 170 days the differences in weight gain were compensated. Faecal egg output was higher in the 200 000 groups only in the first period of patency, thereafter the calves in the 20 000 groups produced more. No obvious differences between the two infection levels were observed with regard to the haematological data. In the low-infected groups worm counts were only slightly lower when the results after 28 days were compared with those after 56 days. Also, the worm numbers after 14 days were almost equal to those after 84 days, while at 140 days 1 animal still had the same number, the other one had lost its worm burden. In the high-infected groups the worm loss was much quicker. After 28 days a great part of the population had already been lost. Obviously, at the 200 000 level the reaction of the host against the parasite was much stronger. Adult worms were expelled at a higher rate than early 4th-larval stages. In the first experiment worm measurements revealed differences between the length of females, males and spicules of males, these being significantly longer in the low-infected groups. Analysis of the distribution of worms over the small intestine showed that in the low-infected groups worms were mainly restricted to the first 6 metres. In the high-infected groups the worm population was distributed more evenly over the whole small intestine.

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The effect of diet on the establishment and growth of Ostertagia ostertagi in calves.

Three groups of four calves were raised parasite-free. Each group received a different diet. At the moment of infection one group was fed with milk equivalent artificially dried grass, and concentrates, the second with water, grass pellets (16 mm), and concentrates, while the third was fed with water, artificially dried grass, and concentrates. Each animal was infected at an age of 8 weeks with 10,000 larvae of Ostertagia ostertagi and necropsied 28 days later, No significant differences between the groups were observed with regard to the number of worms post-mortem, length of worms, number of eggs per female, or gain of weight of the calves.

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A field trial with a new anthelmintic oxfendazole in naturally infected calves.

In a field trial on a series of 10 farms involving 116 calves the activity of oxfendazole at a dose rate of 2.5 mg/kg against gastro-intestinal helminths was assessed. On the basis of egg counts and larval differentiation before and after treatment it was shown that the compound had excellent activity against the most common nematodes. Although not proven in this trial, there were indications of some activity against inhibited stages of Ostertagia. Activity against Strongyloides was lower, while for Trichuris and Moniezia the infection rates before treatment were too low to draw any definitie conclusions about the activity of oxfendazole against these parasites.

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