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Biomedical subjects

F H Schroeder

Publications and source records attributed to F H Schroeder.

64 records · Page 4Linked to original sources
PubMed1978

EB 33, an epithelial cell line from human prostate carcinoma: a review.

The cell line EB 33 was cultivated from the tissue of a human prostate adenocarcinoma in June 1973. Since that time, efforts have been made by scientists in several laboratories to characterize this line by morphologic, biochemical, endocrinologic, genetic, and immunologic parameters. The great need for a well-defined prostatic cell line for immunologic experimentation warrants a complete review of the subject.

Acid Phosphatase↗
PubMed1978

Heterotransplantation of human prostate carcinoma into nude mice.

Human prostate cancer has been successfully transplanted into nude mice, and our technique is briefly reviewed. Sections of human benign prostatic hypertrophy have also been successfully transplanted, with subsequent cellular multiplication. This technique provides a promising model for laboratory studies of therapy by hormone manipulation.

Animals↗
PubMed1978

Renal cell carcinoma: two new cell lines and a serially transplantable nude mouse tumor (NC 65). Preliminary report.

Two long-term tissue culture cell lines and a serially transplantable tumor line growing on nude mice were obtained from a renal cell adenocarcinoma (NC 65). Results of the preliminary characterization of these tumor lines are presented. These data include tumor and cell morphology and growth characteristics in vivo and in vitro. In the nude mouse the tumor matastases to axillary and inguinal lymph nodes. The slow growth rates in culture and in the nude mice as well as the maintenance of the original histologic picture indicate that this tumor still reflects properties of the original carcinoma. Chromosomal analysis proves the human origin of the cell lines.

Adenocarcinoma↗
PubMed1978

Antigenic properties of a cell line from human prostate carcinoma (EB 33).

Evidence for tumor-specific transplantation antigens of human prostate carcinoma was gained by others from in vivo sensitization. The fact that these antigens have not been detected by in vitro methods prompted us to investigate whether EB 33 cells, originated from a human prostate carcinoma by one of us (F.H.S.), expressed these antigens. Using 3-M potassium chloride extracts of EB 33 cells for immunization of New Zealand white rabbits, we obtained xenogeneic antibodies. Further analysis of their specificity was achieved by indirect immunofluorescence and by measurement of their cytotoxicity in a [51Cr]-release test. Xenogeneic antibodies were cytotoxic for EB 33 cells. However, the extent of cell lysis depended on the passage number of EB 33 target cells, thus reflecting an alteration of the antigenicity of the EB 33 cell population during culture. Formation of nonspecific antibodies could be absorbed with HeLa cells. As HLA were not detectable on EB 33 cells, results obtained from absorption experiments with homogenates of normal and malignant prostate tissue may argue for organ-specific and tumor-related transplantation antigens on EB 33 cells.

Antibodies, Neoplasm↗
PubMed1976

Human prostatic epithelial cells in culture: clonal selection and androgen dependence of cell line EB 33.

The permanent epithelial strain EB 33 was initiated from primary cultures of a human prostatic adenocarcinoma in June 1973. To establish androgen dependence of growth EB 33 cells were grown in various media. The effect of androgen withdrawal was studied by the application of media containing sera that had undergone steroid extraction procedures with dichloromethane and activated charcoal. The growth rate of the original EB 33 population was not influenced by withdrawal or addition of androgenic hormones. There were 111 clones developed by single cell plating of EB 33 cells and 23 clonal lines responded to extracted media with a depression of growth of at least 50 per cent. These findings were verified in growth curve experiments. Testosterone and 5-alpha-dihydrotestosterone slightly accelerated growth but did not fully compensate the depression of cell counts seen in extracted media. These findings may be consistent with hormone dependence of growth of clonal lines developed from the human prostatic epithelial strain EB 33.

Adenocarcinoma↗
PubMed1976

Human prostatic adenoma and carcinoma. Transplantation of cultured cells and primary tissue fragments in "nude" mice.

Tissues from human prostatic carcinomas and adenomas as well as cells from permanent cell lines originating from such tumors were transplanted into the subcutaneous space of "nude" mice. A total of 208 such transplantations were carried out. For transplantation of primary tumor tissue several techniques were used. Transplantation of single pieces of about 50 mg of tissue from carcinomas and adenomas provided the best results. With this technique carcinoma tissue was recovered from five of 10 tumors transplanted into 46 "nude" mice. Carcinoma was found in seven of 25 "nude" mice that were castrated and treated with testosterone but only once in 21 transplantations into castrates without testosterone substitution. This may suggest that heterotransplants of human prostatic carcinoma tissue can be established more easily in testosteronized animals. In the case of adenoma transplants, the histological picture was strikingly similar to the original tumors. Secretory activity was found in some acini, squamous metaplasia in others. Heterotransplantation of cells from permanent cell lines resulted in fast growing carcinomas without similarity to the origianl tumors. Dependence on hormones for growth of EB 33 and MA 160 cells in "nude" mice was not shown.

Animals↗
PubMed1975

Carcinoma of the prostate: a study of 213 patients with stage C tumors treated by total perineal prostatectomy.

The effects of age, histological grade and clinical extension of carcinoma of the prostate are analyzed in a series of 213 patients with stage C carcinomas treated by total perineal prostatectomy. Survival data and the incidence of local recurrence, distant metastases and deaths from cancer of the prostate are the parameters used for comparison with 132 stage B patients, 70 patients with seminal vesicle invasion and data from the literature. Long-term survivors in the young age group and some long-term survivors with high grade lesions are good arguments for the possible curability of early stage C carcinoma of the prostate by total prostatectomy. Correct surgical staging of these tumors by previous pelvic lymphadenectomy is mandatory.

Aged↗