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Biomedical subjects

F Ide

Publications and source records attributed to F Ide.

86 records · Page 5Linked to original sources
PubMed1981

Autoradiographic demonstration of unscheduled DNA synthesis in oral tissues treated with chemical carcinogens in short-term organ culture.

A system in which oral tissues of inbred F344 adult rats and Syrian golden hamster embryos were used in combination with autoradiography was developed for measurement of unscheduled DNA synthesis (UDS). For this, oral mucosa, submandibular gland, tooth germ and mandible in short-term organ cultures were treated with 4-nitroquinoline 1-oxide or N-methyl-N-nitrosourea plus (methyl-3H)thymidine. Significant numbers of silver grains, indicating UDS, were detected over the nuclei of cells of all these tissues except rat salivary gland after treatment with carcinogens. This autoradiographic method is suitable for detection of UDS in oral tissues in conditions mimicking those in vivo. Results obtained in this study indicated a potential use of this system for studies on the mechanism of carcinogenesis at a cellular level comparable to in vivo carcinogenesis studies on oral tissues.

4-Nitroquinoline-1-oxide↗
PubMed1981

An ultrastructural and immunohistochemical study on mandibular lesion of Letterer-Siwe disease.

A mandibular lesion from an infant with Letterer-Siwe disease was studied by electron microscopy and immunohistochemistry. Ultrastructurally, two types of cells were identified; clear and dark cells. Clear cells had indented nuclei and langerhans cell granules, while dark cells were characterized by the occasional presence of a large number of lysosomes. Lysozyme was usually not detectable in proliferating cells with indented nuclei, but it was strongly positive in cells without indented nuclei. The latter also usually contained IgG, although the occurrence of cytoplasmic IgM, A, D and E seemed to be negative in both types of cells. Thus clear cells showed a close resemblance to Langerhans cells and dark cells had features of ordinary histiocytes. Although the intimate relationship between these two types of cells remains obscure, our findings suggest that Letterer-Siwe disease may be a reactive or tumorous condition of a cellular subpopulation of Langerhans cells with a proliferation of differentiated histiocytes.

Antigen-Antibody Reactions↗
PubMed1980

Autoradiographic demonstration of DNA repair synthesis in rat tracheal epithelium treated with chemical carcinogen in vitro.

A system in which tracheal organ culture of inbred F344 rats was used in combination with autoradiography was developed for quantitative measurement of DNA repair synthesis in tracheal epithelial cells. Small sections of trachea in short-term organ culture were treated with various carcinogens plus [methyl-3H]thymidine. Significant numbers of grains, indicating DNA repair, were detected on the nuclei of epithelial cells of tracheal sections treated with carcinogens, and the numbers were proportioned to the concentrations of the carcinogens. The nuclear [methyl-3H]thymidine labeling index (S-phase index) was approximately 0.02, and this did not interfere appreciably with quantitative grain counting. This autoradiographic method is suitable for quantitative measurement of DNA repair synthesis in epithelial cells of the trachea in conditions mimicking those in vivo. These results suggest the potential use of this system for studies on the mechanism of carcinogenesis at a cellular level in keeping with recent biochemical studies on metabolism of carcinogens in respiratory organs in culture. The system may also be useful for screening environmental chemicals suspected of damaging DNA of the respiratory organs in relation to lung carcinogenesis.

4-Nitroquinoline-1-oxide↗
PubMedDate not supplied

Traumatic herniation of the buccal fat pad.

Two new cases of traumatic herniation of the buccal fat pad are presented. The herniated fat pad was repositioned in case 1 and was excised in case 2. There was no recurrence after surgery in each case. Reviewing the literature, the lesion usually occurred in infants or young children under the age of 4 years as a result of traumatic penetration of the buccal mucosa and buccinator muscle with a foreign object.

Accidental Falls↗