Metals and selenium in Sand Martin's plumage.
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Age Factors↗
Biomedical subjects
F Kiss
Publications and source records attributed to F Kiss.
Altered elemental composition of the cell wall of a pseudomonas strain having inducible tolerance mechanism to chromium(VI).
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Cell Membrane↗
Mitochondrial function in cell wall glycoprotein synthesis in Saccharomyces cerevisiae NCYC 625 (Wild type) and [rho(0)] mutants.
We studied phosphopeptidomannans (PPMs) of two Saccharomyces cerevisiae NCYC 625 strains (S. diastaticus): a wild type strain grown aerobically, anaerobically, and in the presence of antimycin and a [rho(0)] mutant grown aerobically and anaerobically. The aerobic wild-type cultures were highly flocculent, but all others were weakly flocculent. Ligands implicated in flocculation of mutants or antimycin-treated cells were not aggregated as much by concanavalin A as were those of the wild type. The [rho(0)] mutants and antimycin-treated cells differ from the wild type in PPM composition and invertase, acid phosphatase, and glucoamylase activities. PPMs extracted from different cells differ in the protein but not in the glycosidic moiety. The PPMs were less stable in mitochondrion-deficient cells than in wild-type cells grown aerobically, and this difference may be attributable to defective mitochondrial function during cell wall synthesis. The reduced flocculation of cells grown in the presence of antimycin, under anaerobiosis, or carrying a [rho(0)] mutation may be the consequence of alterations of PPM structures which are the ligands of lectins, both involved in this cell-cell recognition phenomenon. These respiratory chain alterations also affect peripheral, biologically active glycoproteins such as extracellular enzymes and peripheral PPMs.
Aerobiosis↗
[Current viewpoints on the evaluation of testicular biopsy].
The aim of this study was to analize clinical diagnoses and histopathological reports in 185 cases of male infertility, investigated by testicular biopsies in the period 1993-1997. The classical objective of such investigations has been to clarify either obstructive, or so-called secretory lesions resulting in azoospermia. Histologically normal spermatogenesis was reported in 8 patients (4.3%). Hypospermatogenesis proved to be the most frequent pathologic lesion (69 cases, 37.3%), followed by maturation arrest (61 cases, 33%). Germ cell aplasia (Sertoli-cell-only syndrome) was found in 28 cases (15.1%). Tubular atrophy was the dominant lesion in 5 cases (2.7%). Inflammatory change without other histologic abnormalities was seen only in 1 biopsy (0.5%). Further 13 specimens (7%) showed miscellaneous lesions, including one intratubular germ cell neoplasia. Retrospective histopathologic analysis revealed the coexistence of different basic lesions in 43 of our cases (21.7%). According to the novel requirements, pathologists should always report the presence of germ cell forms in the specimen, which are potentially suitable for fertilization. This is essencial because the new methods of testicular spermium extraction and intracytoplasmatic spermium injection offer the chance of assisted fertilization, even for some patients with smaller testicles and slightly elevated FSH levels.
Biopsy↗
[Food allergy in patients with respiratory allergy. Diagnostic possibilities and problems].
UNLABELLED: In the past two years the authors examined 28 patients with abdominal complaints and allergic respiratory symptoms. Detailed internal, gastroenterological, allergological examinations were made. METHODS: 1 skin Prick-test (SPT) with inhalative and nutritive panel 2. measuring of food-specific (gliadin, alpha-lactalbumin beta-lactoglobulin, ovalbumin) IgG-antibody level detecting with ELISA method, 3. leukocyte migration inhibition (LMI) test against the same foodstuffs, 4. histological examination of the stomach and the duodenum especially for mucosal mastocytes (MMCs). RESULTS: 1. SPT was positive in 23/28 patients for inhalative, but in the 5 cases we did not identify any inhalative allergen. The SPT for the main foodstuffs were positive in 18 patients while in 3 other patients there was urtica only for the other antigens. 2. The food-specific IgG-antibody level was increased in 18/27 patients against one or more antigens. The SPTs and the antibody determination showed identity in 8/18 cases. 3. The LMI tests were positive against one or more main food-products in 23/27 cases. There was common positivity in respect of antigens (between LMI test and antibody identification) in 17 cases. Pathological immunological reactions were presented against the same main foodstuffs with at least two methods for flour in 11, for egg in 10 and for milk in 12 patients. Endoscopic examinations were performed in 27 cases. The number of the MMCs were increased in 22/27 patients. After a specific elimination diet open-food challenges were performed and they confirmed the results of the in vitro and in vivo examinations. CONCLUSION: It is common that the respiratory allergic symptoms in atopic patients accompanied with food allergy for the main foodstuffs caused not only more severe respiratory symptoms, but abdominal complaints too. In respect to the many positive LMI tests the late-type hypersensitivity have important pathogenetical role in it. This three methods together define well the main food-products, which can be antigens as well. The examination of the MMCs supports the local disturbance in the immunoregulatory system.
Adolescent↗
[Reproducibility of malignancy grading in prostatic cancers using the Gleason-Böckling system].
Histologic slides from 50 cases of prostatic adenocarcinoma were evaluated by 5 pathologists, in order to test the reproducibility of grading in two systems. Twenty-five needle core biopsies and 25 surgical (adenomectomy) specimens were graded in two sessions, according to the histomorphologic criteria of Gleason and Böcking. The results were analyzed by the kappa statistics. In surgical specimens, there were no significant differences in the interobserver reproducibility of microscopically assessed categories. In needle biopsies, however, Gleason's primary pattern (62%, kappa = 0.42), and Böcking's histological pattern (63%, kappa = 0.37) showed the highest level of agreement. Among the computed (derivated) classification terms, those consisting of only 3 groups (Gleason grouping, kappa = 0.39; Böcking grade, kappa = 0.39) proved to be better reproducible than the corresponding score values (p < 0.05). When compressing both systems into two grades (high and "non-high"), reproducibility was improved (kappa = 0.52). For a substantial improvement of grading results, more accurate grade definitions, continuing training and regular consultation of pathologists are necessary. Based on the results obtained by intraobserver analysis we conclude that kappa statistics is of limited value when analyzing the role of individual experience at grading reproducibility.
Adenocarcinoma↗
Decomposition of cisplatin in aqueous solutions containing chlorides by ultrasonic energy and light.
Decomposition of cisplatin in an aqueous 0.15 M chloride solution by diffuse daylight, by monochromatic light, and by ultrasonic energy was studied using a new HPLC method. The relative sensitivity of cisplatin to light copies the curve of the absorption spectrum of cisplatin with maximum at ca. 300 nm; however, any light below 500 nm must be avoided. Sonication also leads to a rapid decomposition of cisplatin. Irradiation by diffuse daylight or sonication of the cisplatin solutions for 5 min leads to ca. 1% amminetrichloroplatinate(II). Using HPLC analysis with diode-array spectrophotometric detection, the main decomposition product was identified as amminetrichloroplatinate(II).
Antineoplastic Agents↗
Investigation of chromium (VI) tolerant bacteria.
Pseudomonas strains were isolated from a heavy metal contaminated sludge. Some of the strains grew on chromium (VI) containing medium, and others were inhibited in their growth. Cells were able to remove chromium from the medium. This ability was independent of the rate of their growth. The concentration of chromium (VI) of the media decreased with 15-20 ppm in presence of 10(9) cells. The chromium (VI) was converted into a reduced form of chromium by bacteria. The reduced form of chromium was bound in the cell wall. The bound form of chromium could be recovered more or less with different chemicals. The best percentages of recovery were achieved with NaOH solution.
Biological Transport, Active↗
Specific reduction of wheat storage proteins by thioredoxin h.
Gliadins and glutenins, the major storage proteins of wheat endosperm (Triticum durum, Desf. cv Monroe), were reduced in vitro by the NADP/thioredoxin system (NADPH, NADP-thioredoxin reductase and thioredoxin; in plants, the h type) from either the same source or the bacterium Escherichia coli. A more limited reduction of certain members of these protein groups was achieved with the reduced form of glutathione or glutaredoxin, a protein known to replace thioredoxin in certain bacterial and mammalian enzyme systems but not known to occur in higher plants. Endosperm extracts contained the enzymes necessary to reduce NADP by the oxidative pentose phosphate pathway (hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase). The gliadins and glutenins were also reduced in vivo during germination--an event that accompanied their proteolytic breakdown. The results suggest that thioredoxin, reduced by NADPH generated via the oxidative pentose phosphate pathway, functions as a signal in germination to enhance metabolic processes such as the mobilization of storage proteins and, as found earlier, the activation of enzymes.
Germination↗
Separation of some platinum(II) complexes by ionic strength gradient on a solvent-generated ion-exchange sorbent.
The compatibility of ionic strength gradient with solvent-generated ion-exchange chromatography on an octadecylsilica sorbent was proven for a mobile phase containing octanesulphonate. Only a slight baseline shift was observed during the gradient of the phosphate buffer, even at 210 nm. An equilibration time of 3 min between the runs was sufficient to obtain retention times with a reproducibility better than 1%. The compounds separated were cisplatin, carboplatin and related neutral and cationic platinum(II) complexes, including transplatin and the aquation products of cisplatin.
Carboplatin↗
Redox active sulfhydryls are required for fructose 2,6-bisphosphate activation of plant pyrophosphate fructose-6-phosphate 1-phosphotransferase.
The classical, alpha/beta-subunit form (Q2) of green tomato pyrophosphate fructose-6-phosphate 1-phosphotransferase (PFP, EC 2.7.1.90), a cytosolic enzyme functional in carbohydrate metabolism, was rapidly inactivated on incubation with the oxidant 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB). Analysis of the DTNB-treated sample by a fluorescence procedure revealed that inactivation was accompanied by oxidation of sulfhydryl groups, primarily on the alpha-subunit. Phosphate metabolites--fructose 2,6-bisphosphate, fructose 1,6-bisphosphate, Pi, and PPi--protected against DTNB inactivation to varying degrees. The Km values for fructose 6-phosphate and PPi were not changed by DTNB treatment, but the capability for activation by fructose 2,6-bisphosphate was severely diminished. The oxidative inactivation of PFP was reversed by dithiothreitol, but not by monothiols (reduced glutathione or beta-mercaptoethanol). Reactivation was accompanied by restoration of the ability to undergo activation by fructose 2,6-bisphosphate. The findings suggest that sulfhydryl groups are essential for the activation of PFP by fructose 2,6-bisphosphate and raise the possibility that a reversible change in their redox status can take place under certain conditions. Evidence that this is the case was obtained with a preparation from wheat flour which, in the absence of an added oxidant, required reduction by a dithiol for activation by fructose 2,6-bisphosphate (dithiothreitol and reduced thioredoxin h).
Dithionitrobenzoic Acid↗
HPLC analysis of platinum cytostatics.
High performance liquid chromatography (HPLC) with diode array detector (DAD) was applied for the separation of platinum cytostatics (cisplatin, carboplatin and oxo-carboplatin). Their stabilities were studied in water and the influence of chloride anions, pH, temperature and time was discussed and rate constants of the aquation reactions at different conditions were calculated. Ligand exchange reactions cisplatin----carboplatin were evaluated using HPLC-DAD system. Detection limits for all studied cytostatics were determined and extraction recoveries for carboplatin in clinical samples (serum) were demonstrated for a wide range of concentrations together with the relative standard deviations. The solid-phase extraction procedure was recommended for clinical sample analysis represented by a model mixture and real patients' samples.
Carboplatin↗
Pyrophosphate Fructose-6-P 1-Phosphotransferase from Tomato Fruit : Evidence for Change during Ripening.
Three forms of pyrophosphate fructose-6-phosphate 1-phosphotransferase (PFP) were purified from both green and red tomato (Lycopersicon esculentum) fruit: (a) a classical form (designated Q(2)) containing alpha- (66 kilodalton) and beta- (60 kilodalton) subunits; (b) a form (Q(1)) containing a beta-doublet subunit; and (c) a form (Q(0)) that appeared to contain a beta-singlet subunit. Several lines of evidence suggested that the different forms occur under physiological conditions. Q(2) was purified to apparent electrophoretic homogeneity; Q(1) and Q(0) were highly purified, but not to homogeneity. The distribution of the PFP forms from red (versus green) tomato was: Q(2), 29% (90%); Q(1), 47% (6%); and Q(0), 24% (4%). The major difference distinguishing the red from the green tomato enzymes was the fructose-2,6-bisphosphate (Fru-2,6-P(2))-induced change in K(m) for fructose-6-phosphate (Fru-6-P), the ;green forms' showing markedly enhanced affinity on activation (K(m) decrease of 7-9-fold) and the ;red forms' showing either little change (Q(0), Q(1)) or a relatively small (2.5-fold) affinity increase (Q(2)). The results extend our earlier findings with carrot root to another tissue and indicate that forms of PFP showing low or no affinity increase for Fru 6-P on activation by Fru-2,6-P(2) (here Q(1) and Q(0)) are associated with sugar storage, whereas the classical form (Q(2)), which shows a pronounced affinity increase, is more important for starch storage.
Journal Article↗
[Unity of diagnostic pathology, continuing education and research at our institute].
Authors give a brief view on the activity in Institute of Pathology and Histopathology of Post-graduate University of Medicine. In its frame, close relation of diagnostic, post-graduate education and research activity is illustrated by examples of different fields (intestinal, cardiac, vascular system, respiratory tract, organ of locomotion, urological diseases, electronmicroscopic tumor diagnostics, endocrinology). Importance of modern morphological methods and their place both in practical and scientific activity are dealt with.
Cardiovascular Diseases↗
Papillary serous cystadenoma of the testis.
A case is presented of a 50-year old man with a unilocular cystic intratesticular tumour exhibiting the morphological features demanded from WHO for the diagnosis of serous papillary cystadenoma of the ovary. Keratin filaments could be demonstrated in the cyst lining and papillae covering cells by means of PAP-technique; AFP and SP-1 were lacking. The epithelial cells of the tumour showed a lectin binding pattern (WGA, UEA-I, PNA, Con A, PSA, LCA, RCA) different from the epithelium of rete testis and epididymis. We intend to classify our tumour as the male analogue of the respective ovarian growth.
Cystadenoma↗
Cytokeratin expression in experimental murine rhabdomyosarcomas. Intermediate filament pattern in original tumors, allotransplants, cell culture and re-established tumors from cell culture.
Soft tissue sarcomas were induced by 20-methylcholanthrene in NMRI-mice. The tumors were characterized as rhabdomyosarcomas by light and electron microscopy as well as immunohistochemistry (vimentin, desmin and myoglobin expression). Cytokeratins could be demonstrated by a panel of different poly- and monoclonal antibodies in original rhabdomyosarcomas, their allotransplants and the re-established tumors from cell culture in nude mice. The cytokeratin positive tumor cells were arranged in small clusters and/or haphazardly single dispersed in the rhabdomyosarcomas. By means of monoclonal antibodies cytokeratins No. 8 and No. 19 could be evidenced and cytokeratin No. 18 could be made probably. Behind the background of cytokeratin expression in developing fetal cross striated muscle cells our findings are discussed as a reminiscence of embryonal muscle development in these tumors. The significance of cytokeratin expression in rhabdomyosarcomas for diagnostic histopathology is emphasized.
Animals↗
Antitumor activity and cross-resistance studies with Pt-ascorbato complexes.
Two ascorbatoplatinum complexes, cis-diammineascorbatoplatinum(II) (AMA) and cis-bis(ascorbato)-trans-diaminocyclohexaneplatinum(II) (CHA), were tested for antitumor activity in vivo on P388 leukemia and in vitro in suspension culture and soft agar assay. Sensitive line of L1210 and sublines with resistance induced against cis-diamminedichloroplatinum(II) (DDP) and two derivatives of trans-1,2-diaminocyclohexane (DACH) were used for the in vitro tests. DNA synthesis inhibition in both sensitive and resistant cells was tested. The results are compared with DDP and DACH-Pt(II)-4-carboxyphtalate (TMA). Both tested complexes proved their antitumor activity in our experimental systems. The CHA complex was more effective than AMA and its effectiveness is comparable with that of DDP and TMA. Cross-resistance was found between DDP and AMA as well as TMA and CHA. There was no cross-resistance between DDP versus CHA, and TMA versus AMA.
Animals↗
Effect of insulin on the glycogen content of the rat small intestinal mucosa.
The authors examined the quantitative changes in the glycogen content in rat's small intestinal mucosa under the effect of insulin. They found that glycogen content increases significantly during the first 60 minutes, then it decreases gradually, and regains the control value only after 6 hours. It appears that as regards its metabolic activity, the small intestinal mucosa is not an insulin resistant organ.
Animals↗