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Biomedical subjects

F Klein

Publications and source records attributed to F Klein.

At least 109 records · Page 6Linked to original sources
PubMed1979

Leiomyosarcoma of the pulmonary artery. A light and electronmicroscopical study.

A 25-year-old man with chest pain and shortness of breath was found to have a primary sarcoma of the pumonary artery. On light- and electronmicroscopy and immunofluorescence microscopy the lesion was found to be composed of cells of smooth muscle origin. It was diagnosed as leiomyosarcoma. The cross and microscopic features of the tumor are described and the morphologic characteristics of previously reported vascular sarcomas are briefly reviewed.

Adult↗
PubMed1979

Large-scale production and concentration of human lymphoid interferon.

A stable and predictable production system is described for pilot plant quantities (milligram) of human lymphoid interferon, using suspension culture of an African Burkitt's lymphoma derived cell line Namalva with induction by Newcastle disease virus, B-1 strain. Cell cultures were grown in impeller-driven 50-liter fermentors with dilution of the postinduction culture using serum-free medium. High levels of dissolved oxygen were necessary for optimum cell growth. A total of 4,207 liters of interferon culture was produced in a series of 116 fermentor runs. An average yield of 3.5 log(10) international units of interferon per ml was realized before processing. Trichloroacetic acid was used to precipitate the interferon. An average of 3.35 log(10) international units of interferon per ml was recovered in the final nonpurified product.

Burkitt Lymphoma↗
PubMed1979

[Primary spindle cell sarcoma of the pulmonary artery].

It is reported on a patient with a primary, mainly spindle-cell sarcoma of the left pulmonary artery. It is a case of a centrally located tumor, which caused a subtotal occlusion of the truncus pulmonalis and a complete occlusion of the left pulmonary artery. The diagnostical and therapeutical methods with their results and possible complications are discussed. The indication for an operation is demonstrated. In the pertinent literature hitherto 35 cases of this disease are reported.

Adult↗
PubMed1979

[Arylsulfatase A and sphingomyelinase activities in the normal sciatic nerve of the rat, and in proximal and distal fragments].

We measured the protein, arylsulfatase A and sphingomyelinase activities in the total normal rat sciatic nerve and in the proximal and distal stumps. The protein level of the whole sciatic nerve (about 8 % of fresh weight) is similar to the levels of the stumps. Arylsulfatase A activity, in the total sciatic nerve as well as in the stumps is more important by gram of fresh weight than the sphingomyelinase activity. Both enzymes activities, by gram of fresh weight, are more important in the proximal stumps than in the distal ones.

Animals↗
PubMed1979

[Haemangioma capillare of the pleura with ipsilateral haemopneumothorax (author's transl)].

1. The article introduces a patient with left-side haemangioma capillare of the pleura parietalis with haemopneumothorax and ipsilateral cystic changes of the left upper lobe of the l,ng. 2. The aetiopathogenesis of the disease is discussed as an abortive type of Rendu-Osler-Weber disease (hereditary haemorrhagic telangiectasia), the diagnosis being based exclusively on histological findings as the only possibility of diagnosis. 3. The therapeutic approach is discussed.

Adult↗
PubMed1978

[Fatty acid distribution of the normal sciatic nerve lipids in the rat].

We isolated and purified the main lipids of the rat sciatic nerve. After methanolysis, fatty acids were isolated and purified by thin layer chromatography, and studied by gas chromatography. C 16 and C 18 fatty acids are the most abundant. Among the long-chain fatty acids, arachidonic acid (20:4) is present in some lipids; highly desaturated fatty acids in C 22 and C 24 are also present. In general, the fatty acids are highly saturated; cholesterol esters and ethanolamine phosphoglyceride fatty acids are highly unsaturated.

Animals↗
PubMed1978

Large-scale production and concentration of infectious Epstein-Barr virus.

Infectious Epstein-Barr virus (EBV) was produced from suspension cultures of P3HR-1 cells. A protocol for the scaled-up production and concentration of the virus was developed. Virus from the culture fluid was concentrated by continuous-flow pelletization or continuous flow with banding in sucrose. EBV prepared by pelletization yielded 1.7 X 10(4) infectious units/ml (100 X concentration) and less than 3.4 X 10(7) EVB particles/ml (1,000 X concentration), whereas EBV prepared by banding yielded 4.6 X 10(3) infectious units/ml (100 X concentration) aand 1.3 X 10(8) EBV particles 1 ml (1,000 X concentration). The majority of the virus particles observed were "empty" membrane-associated particles. A statistical test of the correlation between infectivity and virus particle count was made.

Cell Division↗
PubMed1977

Cholesterol determinations. A comparative study of methods with special reference to enzymatic procedures.

Two enzymatic methods for the determination of cholesterol in serum have been modified for automatic use. These methods utilize the colour reactions according to Kageyama or Trinder to measure the hydrogen peroxide resulting from the enzymatic reaction. The procedures have been compared with the classical methods according to Abell and to Huang. Details regarding the procedures are given. Comparisons made during routine analysis of a large number of different sera have been expressed in the form of regression equations. The standard errors of the methods are given along with the standard deviations for differences between methods. Some conversions of values from one method into another are also given. It appears that the automated Kageyama method can be used for large scale practice. The manual Kageyama method is not very satisfactory because of its large experimental error. A disadvantage of the Kageyama methods is the necessity of having blank corrections for haemolytic sera. The Trinder method avoids the problem and has some additional advantages.

Autoanalysis↗