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F M Debruyne

Publications and source records attributed to F M Debruyne.

At least 433 records · Page 24Linked to original sources
PubMed1986

Intermediate filament proteins as tissue specific markers in normal and malignant urological tissues.

Immunocytochemical techniques have become valuable tools in many fields of clinical pathology and medical research. Especially the development of highly specific (monoclonal) antibodies to a large variety of tissue antigens has in recent years led to the establishment of sensitive tissue markers. One of the most promising types of tissue specific markers so far is represented by the intermediate filament proteins. Since the findings of this rapidly expanding field are also being applied in urology, we have reviewed the current data in order to describe the new insights in tumor biology and histogenesis, as well as their application in diagnostic pathology.

Animals↗
PubMed1986

Prostaglandins in urology.

This article gives a review of the current place of the different prostaglandins in the urogenital tract and urological pathology. Clinical as well as experimental data are analyzed. The conclusion is that prostaglandins play an important role in the physiology and pathophysiology of each organ of the urogenital tract although no direct clinical applications of prostaglandins or prostaglandin-inhibitors result from our current knowledge and experiences with these substances.

Epididymis↗
PubMed1986

Application of antibodies to intermediate filament proteins as tissue-specific probes in the flow cytometric analysis of complex tumors.

The flow cytometric (FCM) analysis of carcinomas is often hampered by the presence of stromal and inflammatory cells in the cell suspensions obtained from such neoplasms. Therefore, an FCM method was developed to distinguish epithelial from nonepithelial cells by using polyclonal and monoclonal antibodies to (cyto)keratins, the epithelial type of intermediate filament proteins. Using a model system of cultured bladder carcinoma (T24) and leukemia (MOLT-4) cells, we tested our hypothesis and procedures by labeling cell mixtures with these antibodies. After incubation with an appropriate intermediate filament antibody and propidium iodide staining, the DNA content and distribution of T24 cells could be analyzed separately from MOLT-4 cells. When applied to cell suspensions of endometrial carcinomas, bladder carcinomas and Grawitz tumors, only the epithelial (primarily carcinoma) cells were stained for cytokeratin; these cells could thus be analyzed separately from stromal, inflammatory and other nonepithelial cells. In this way, a more accurate FCM analysis of the malignant fraction within a tumor can be achieved.

Adenocarcinoma↗
PubMed1986

[Urologic tumors in old age].

Malignant urological diseases account for an important part of all malignant tumors in the elderly. It is obvious that in the elderly the same diagnostic tools should be applied for diagnosing and staging of a tumor. This screening is important to select those tumors which are accessible for curative therapy. Generally this is only possible when the tumor is limited within the borders of the organ. In these cases curative surgery with acceptable morbidity and minimal mortality is also possible in the elderly. Radical nephrectomy, prostatectomy and cystectomy are applied when a life-expectancy of approximately 10 years is present. When metastasized, only palliative measurements are possible. The quality of life now plays a major role and is more important than the survival time. This has to be taken into account when considering palliative treatment of any kind.

Aged↗
PubMed1985

Tissue-specific markers in flow cytometry of urological cancers: cytokeratins in bladder carcinoma.

Thirty-eight transitional-cell carcinomas (TCC) were analyzed by flow cytometry (FCM) using propidium iodide for DNA analysis and antibodies to cytokeratin by indirect immunofluorescence. By means of two-dimensional FCM analysis, cytokeratin-positive tumor cells could be analysed separately from cytokeratin-negative stromal and inflammatory cells. This resulted in an 18% increase in sensitivity of FCM detection of aneuploidy (10/38 samples with one-parameter DNA analysis versus 15/38 samples with two-parameter DNA and cytokeratin analysis). In addition, S-phase could be determined in the 15 aneuploid samples by means of two-parameter analysis where this was not possible using only DNA content because of the overlap of diploid and aneuploid populations. FCM analysis allowed quantification of the percentage of tumor cells expressing cytokeratin 18 which has previously been shown to correlate quantitatively with higher grade, higher stage TCC. The quantitative measurement of tumor-cell expression of cytokeratin 18 by FCM analysis appears to provide additional information of potential prognostic value, independent of tumor-cell ploidy and proliferative fractions.

DNA↗
PubMed1985

Cell DNA content--correlation with clonogenicity in the human tumour cloning system (HTCS).

Thirty-six ovarian and renal-cell tumours were analyzed by flow cytometry (FCM) for DNA content and in parallel were assayed for colony formation in a human tumour cloning system (HTCS). While 15/19 (79%) tumours with an abnormal (aneuploid) DNA stemline formed colonies in the HTCS, only 2/17 (12%) of diploid tumours formed colonies. All samples contained tumour cells as assessed by routine cytological examination. The capacity to form colonies in the HTCS was not correlated in these tumours with grade or stage of disease or tumour type. The level of aneuploidy expressed as the FCM DNA index did not correlate with the cloning efficiency in HTCS. These findings suggest that tumour growth in the HTCS reflects a biologically important potential, related in at least some tumours to an abnormal DNA stemline.

Carcinoma, Renal Cell↗
PubMed1985

Repeated urine cell culture in soft agar: potential role in follow-up of patients with transitional cell carcinoma.

To evaluate the persistence of cells with clonogenic properties in patients being treated for transitional cell carcinoma, 272 urine samples were collected from 75 patients and cultured in a double layer soft agar "cloning" system. The development of colonies was evaluated with growth curves based on repeated colony counting with an Omnicon automated colony counter at regular time intervals. Forty-eight patients had at least one evaluable culture. Comparing the results of colony development in culture with the clinical evaluation of the patients, 9 patients had a histologically proven recurrence preceded or accompanied by tumor colony growth in urine culture. One patient had tumor recurrence with growth negative urine culture (false negative). Fifteen patients have had growth negative urine culture with a negative follow-up (mean 19.5 months). Twenty-one patients have had growth positive urine cell cultures with no recurrence in their follow-up (mean 18.7 months). Although the follow-up times are at present relatively short, the present study suggests that repeated soft agar urine culture of patients with low-grade, low-stage bladder carcinoma may provide a means for identifying those patients at a higher risk for recurrence/progression of their disease.

Agar↗
PubMed1985

Ciprofloxacin distribution in prostatic tissue and fluid following oral administration.

The penetration of ciprofloxacin into prostatic tissue was studied following oral administration of 500 mg either once or repeatedly in 12-hourly intervals. Following single administration ciprofloxacin was rapidly absorbed from the gastrointestinal tract peaking 1-2 h after administration. Elimination from serum was slow, the half life being 4.3 h. No significant rise in serum concentrations was noticed following repeated administration. Ciprofloxacin was concentrated in the prostatic tissue, levels being on average twice as high as the corresponding serum concentrations. The ratios between prostate and serum levels following single and repeated administration were 227 and 214%, respectively. Intraindividual analysis of prostate concentrations in different areas of the prostatic gland revealed a homogeneous distribution within the prostate. Penetration of ciprofloxacin into prostatic fluid was studied in 11 patients 2-4.5 h after administration. At these points ratios between prostatic fluid and serum ranged between 1.5 and 450%.

Administration, Oral↗
PubMed1985

Current concepts in the management of paratesticular rhabdomyosarcoma.

Paratesticular rhabdomyosarcoma is a rare malignant disease most commonly seen in infants and children. In contrast with rhabdomyosarcomas in sites other than the genitourinary tract, paratesticular rhabdomyosarcomas tend to metastasize via a lymphatic route. This influences the therapeutic approach in which surgery, chemotherapy and, in selected cases, radiation therapy play an essential role. On hand of 7 of our own patients, uniformly treated at one institution, a review of the current approach of paratesticular rhabdomyosarcomas with excellent therapeutic results is given.

Adolescent↗
PubMed1985

Squamous and transitional elements in rat bladder carcinomas induced by N-butyl-N-4-hydroxybutyl-nitrosamine (BBN). A study of cytokeratin expression.

Three hundred rat bladders bearing tumors induced by N-butyl-N-4-(OH)butyl-nitrosamine (BBN) were examined by routine histologic study and immunohistochemical staining of intermediate filament types. Smaller lesions were similar to human urothelial dysplasia histologically and immunohistochemically. Progression of the lesions demonstrated large exophytic papillomas with extensive endophytic epithelial growth into abundant stroma. These lesions showed increasing predominance of squamous over transitional elements. Immunohistochemical findings confirmed these results and also demonstrated that morphologically indifferent cells, even in early lesions, express heavier cytokeratins characteristic of keratinizing squamous epithelium. These results demonstrate that BBN-induced bladder tumors show marked quantitative and qualitative differences from the most common, purely transitional, human bladder carcinomas. However, the development in BBN-treated rat bladders of two tumor types, squamous and transitional, from an altered urothelium may serve as an attractive model for further study of the molecular genetics of keratin expression.

Animals↗