PubMed Health⌕ Search

Biomedical subjects

F M Debruyne

Publications and source records attributed to F M Debruyne.

At least 451 records · Page 25Linked to original sources
PubMed1984

Radioimmunoassay (RIA) for prostatic acid phosphatase in patients with prostatic carcinoma.

In recent years radioimmunological measurements of prostatic acid phosphatase have been proposed for the diagnosis, follow-up and prognosis of prostatic carcinoma. The possibility of screening male populations at risk has even been suggested. The present paper deals with the current position of this method. We studied the specificity and sensitivity of the radioimmunoassay (RIA) for prostatic acid phosphatase in three groups of patients: a normal population, patients with benign prostatic hyperplasia, and patients with untreated prostatic carcinoma. The conclusions of this study are that the RIA is a specific method but the sensitivity is much too low to use the RIA for diagnosis and screening of patients. Comparison with the enzymatic method indicates that under good laboratory conditions the latter is preferable except for patients with metastatic disease and normal enzymatic acid phosphatases.

Acid Phosphatase↗
PubMed1984

Chylous ascites after retroperitoneal lymph node dissection.

Two cases of chylous ascites secondary to lymph node dissection for testicular tumors are presented. The diagnosis is confirmed by analysis of the fluid obtained by paracentesis, revealing fat globules, a positive staining with Sudan III, a smear showing mainly lymphocytes, and a high fat content. Treatment consisted of a medium chain triglycerides (MCT) diet, furosemide, and salt restriction.

Adult↗
PubMed1984

Rational antimicrobial prophylaxis on a urological ward.

The development of a policy for antimicrobial use on a urological ward is presented. The introduction of guidelines for rational antimicrobial use and the monitoring of their effect over a four-year period are described. As a result of the implementation of this policy, a reduction of over 60 percent in antibiotic consumption on the urological ward was achieved. Without interference in the quality of patient care, as determined by the incidence of postoperative wound infections and adverse effects, the overall number of patients treated with antimicrobial agents was reduced 50 percent, but proved to be age dependent. In addition, costs were diminished 20 percent. These results were achieved by intensive collaboration between the Departments of Urology and Clinical Pharmacy.

Adult↗
PubMed1984

[Synchronous and metachronous bilateral hypernephroid renal carcinomas].

In 1.4-3.9% Grawitz tumours occur bilaterally, either synchronously or metachronously. Three patients with a bilateral Grawitz tumour are described, two with a synchronous and one with a metachronous one. Epidemiology, pathology, clinical symptoms, radiodiagnostics, therapy and prognosis of the bilateral Grawitz tumours are discussed.

Adenocarcinoma↗
PubMed1983

Patterns of tumor colony development over time in soft-agar culture.

Human tumors were cultured by the two-layer soft-agar technique and the time course of tumor colony development was evaluated during periods of up to 6 weeks in culture. All colony counting was performed with an automated tumor colony counter (Omnicon; Bausch and Lomb, Inc, Rochester, NY, USA). This instrument provided colony counts per culture plate in six size categories from greater than 60 microns diameter colonies to greater than 149 microns diameter colonies. Six to 24 culture plates were used for each "growth curve", generally 24. Control (non-drug-treated) cultures were obtained from 117 tumors, of which 25 also provided enough cells to allow evaluation of the time course of colony development after exposure to cytostatic agents. The development of colonies in non-drug-treated plates usually demonstrated a lag phase, a logarithmic growth phase to maximum colony development and a subsequent deterioration of colonies. In spite of clumps seeded into the agar, real colony growth could be recognized by frequent colony counting of culture dishes, although the temporal patterns of growth were sometimes different if pure single-cell suspensions were compared with suspensions containing clumps from the same tumor. Drug pre-incubation caused changes in the temporal pattern of colony growth as well as in the total number of colonies. Some cultures showed drug sensitivity when evaluated at certain time points while evaluation at later time points showed only borderline drug effect or none at all. The potential utility of tumor colony growth curves in the clinical applications of tumor colony cultures is discussed.

Agar↗
PubMed1983

In-use evaluation of the Omnicon automated tumor colony counter.

The reproducibility and accuracy of the Omnicon (Bausch and Lomb Inc., Rochester, NY) automated tumor colony counter for counting tumor colonies growing in double layer soft agar is evaluated and the reproducibility is compared with manual tumor colony counting. Replicate within day run-to-run colony counts of the Omnicon show a median correlation coefficient (r) of greater than 0.985, and day-to-day median r of greater than 0.980. In contrast, for manual colony counting, the best intra-observer reproducibility achieved is a r of 0.943 and the best inter-observer reproducibility is a r of 0.831. Analysis of results from individual culture plates counted by the Omnicon on 5 separate days shows a median coefficient of variation of 10% with 77% of the culture dishes showing coefficients of variation of colony counts over 5 days of less than 20%. Counting of culture plates during incubation shows that the Omnicon is counting tumor colonies developing after plating of a single cell suspension.

Animals↗
PubMed1983

Bladder neck incision in the female: a hazardous procedure?

Anterior bladder neck incision was carried out in 32 female patients suffering from difficult, mostly incomplete, bladder emptying. All patients underwent a full urodynamic investigation with voiding cystourethrography and endoscopy. Detrusor failure was observed in 20 patients; in the remainder, a mechanical iatrogenic bladder neck obstruction was held responsible for inefficient voiding. Twenty-eight patients (56%) benefited in some degree from the procedure. Six were submitted to a repeat incision and two of them developed severe stress incontinence later on. As the results are far from promising, intermittent self-catheterisation may be proposed in some cases as an alternative to bladder neck incision for fear of incontinence. The urodynamic findings and the results of bladder neck incision, which are very different in the two sexes, are analysed. The poorer results in the female are presumably due to the decompensated state of the bladder, mostly responsible for incomplete bladder evacuation.

Adult↗
PubMed1983

Extended bladder neck incision for outflow obstruction in male patients.

Thirty-two male patients with mechanical or functional bladder outlet obstruction were treated by bilateral endoscopic bladder neck incision. In three cases a repeat incision was necessary. Subjective cure or improvement was obtained in 81%. Objective evaluation, including uroflowmetry and residual urine measurements, showed a similar improvement. Bladder neck incision takes only a few minutes, is simple and less traumatic than transurethral resection. The aetiology of impaired micturition and the value of urodynamic assessment are discussed and some attention devoted to the problem of post-operative retrograde ejaculation.

Adult↗
PubMed1983

Lymphography in the management of urologic tumors. Radiological-pathological correlation.

Of 59 patients undergoing lymphography as part of staging for urologic tumor (bladder carcinoma, prostate carcinoma, seminomatous and nonseminomatous testicular tumors), histological material was obtained in 38. Lymphography was shown to have a specificity of 95%, a sensitivity of 81%, and an overall accuracy of 89%. When lymph node regions were considered, the specificity was 99.2%, sensitivity 59%, and overall accuracy 91%. Causes of discrepancies between radiography and histology are evaluated and receiver operating characteristic curves are demonstrated.

Adult↗
PubMed1983

Soft-agar cultures of transitional cell carcinoma colonies from urine, irrigation fluids and tumor samples.

Soft-agar cultures of transitional cell carcinoma from urine, irrigation fluid and transurethral resection solid tumor specimens show good colony growth. Growth of tumor colonies produced from urine was adequate to evaluate the presence of a viable tumor, since 12 of 18 noninfected cultures showed growth. The number of colonies produced was adequate to evaluate the sensitivity or resistance to chemotherapy agents in only 3 of 18 cultures. For irrigation fluid, similar results were obtained: 9 of 17 noninfected cultures showed growth, while only 3 of 17 were adequate for drug-sensitivity evaluation. For the evaluation of sensitivity or resistance, tumor cell suspensions were most appropriate, 7 of 25 noninfected cultures showed 30 or more colonies/dish, whereas 17 of 25 showed growth. With the possibility of obtaining growth of tumor cells derived from urine, a prospective study is proposed to define the value of repeated urine cultures in monitoring the status of the urothelium of patients treated for transitional cell carcinoma.

Agar↗