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Biomedical subjects

F M Scott

Publications and source records attributed to F M Scott.

At least 37 records · Page 2Linked to original sources
PubMed1990

Rotavirus serotypes 6 and 10 predominate in cattle.

Calf fecal rotavirus strains were serotyped in enzyme-linked immunosorbent assays, using monoclonal antibodies to the VP7s of serotypes 1, 2, 3, 5, and 6 and to the VP4 of B223 (designated serotype 10). Sixty-six percent of 162 samples were typed as serotype 6, and 7% were serotyped as serotype 10. Most of the untyped strains did not react with a monoclonal antibody directed to a common VP7 epitope, indicating insufficient virus present in the samples. However, seven untyped samples that did react with this antibody were adapted to culture and typed, and six of these also proved to belong to serotype 6 or 10. Two of these viruses belonged to a monotype within serotype 6 that did not react with the serotype 6 monoclonal antibody. The seventh isolate reacted in cross-neutralization tests with serotype 8 viruses. Bovine rotaviruses from the United Kingdom, Federal Republic of Germany, and Japan that had been shown previously to be distinct from serotype 6 were compared in neutralization tests with B223 from the United States. These viruses proved to be a closely reacting group distinct from all other rotavirus serotypes, justifying the establishment of serotype 10 as the second major type of bovine rotavirus.

Animals↗
PubMed1989

A serological comparison of bovine coronavirus strains.

Two bovine coronavirus (BCV) strains from diarrheic calf faeces were adapted to grow in HRT 18 cells and compared in immunofluorescence (IF), haemagglutination inhibition (HAI) and neutralisation (NT) tests with three other strains of BCV and a human coronavirus (HCV) strain obtained from other laboratories. Polyclonal antisera against these 6 viruses were raised in rabbits. No significant differences between viruses were detected by IF. In the HAI test the HCV strain was distinguishable from the 5 BCV strains and serological variation between the BCV strains was shown. HCV could be distinguished by NT test, but all BCV isolates were similar. Two monoclonal antibodies prepared against one of the BCV strains distinguished HCV in all three assays, and detected varying relationships between BCV strains.

Animals↗
PubMed1987

Appearance of plasminogen activator activity during a synchronous cycle of a rat adenocarcinoma cell line, PA-III.

A study of the appearance of plasminogen activators during the cell cycle of a rat prostate adenocarcinoma cell line, PA-III, was undertaken. After release from a hydroxyurea (HU) block, the length of the cell cycle was determined and found to be 20-25 h, with the S-G2-M portions comprising approx. 10-15 h and the G1 phase occurring over a similar 10-15-h period. Assays for tissue-like plasminogen activator (t-PA) and urokinase-like plasminogen activator (u-PA) in cell-conditioned medium revealed an increased appearance of both enzymes shortly after the S phase of the cycle. The pattern of production of these activators remained the same under differing conditions of cell synchronization.

Adenocarcinoma↗
PubMed1984

Infection of specific-pathogen free lambs with a herpesvirus isolated from pulmonary adenomatosis.

In two experiments, 18 specific-pathogen free (SPF) lambs were inoculated by several routes with the Scottish strain of caprine herpesvirus 1 (CHV 1). Seventeen of the lambs developed interstitial changes in the lungs ranging from focal cellular infiltration to a widespread proliferative pneumonia. Five weeks after the initial inoculation 3 lambs were given a course of corticosteroid by intravenous injection. Subsequently virus was reisolated from all 3 lambs. Virus was also recovered from one of these lambs on one occasion prior to steroid treatment. It has therefore been established that CHV 1 can cause pneumonia and can be reisolated from infected sheep for at least 6 weeks after infection. It is suggested that CHV 1 might cause a latent infection in sheep which is reactivated following the development of pulmonary adenomatosis.

Animals↗
PubMed1983

Rapid transmission of sheep pulmonary adenomatosis (jaagsiekte) in young lambs. Brief report.

Jaagsiekte, a contagious lung tumour of sheep, was induced within 3-6 weeks in day-old lambs by intratracheal inoculation of SPA lung fluids concentrated by centrifugation. Electronmicroscopic examination of the tumour revealed retrovirus particles whose morphogenesis and morphology support biophysical and immunological findings that suggest a relationship with type B and type D retroviruses.

Animals↗
PubMed1983

Further evidence for a retrovirus as the aetiological agent of sheep pulmonary adenomatosis (jaagsiekte).

The infective agent of jaagsiekte was shown to be present in the fluid which accumulates in the respiratory tract of sheep during the terminal stages of the disease. The fluid also contained reverse transcriptase (RT) activity which showed a clear preference for a ribonucleic acid synthetic template over the corresponding deoxyribonucleic acid template and which utilised the RT specific template/primer poly (2'-0-methylcytidylate) oligodeoxyguanylate. This enzyme activity was associated with a particle which had typical retroviral buoyant densities in a range of gradient media.

Animals↗
PubMed1980

Staphylococcal dermatitis of sheep.

A condition frequently termed "facial" or "periorbital eczema" has been observed in sheep. Studies carried out indicated no viral involvement but a haemolytic Staphylococcus aureus was isolated which produced severe necrotic skin lesions on inoculation into sheep. A more appropriate term for the condition would appear to be staphylococcal dermatitis.

Animals↗
PubMed1980

Virus infections in cattle and sheep in Scotland 1975-1978.

Over the four-year period 1975-78 attempts were made to identify virus in samples from 1231 outbreaks of disease in cattle, and 682 outbreaks of disease in sheep. A virus identification was possible in 26 per cent of these disease outbreaks. The nature and significance of these virus infections is discussed.

Animals↗