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F Meisel-Mikołajczyk

Publications and source records attributed to F Meisel-Mikołajczyk.

At least 73 records · Page 4Linked to original sources
PubMed1991

Antibiotic resistance of Clostridium difficile isolates.

The activity of metronidazole, vancomycin, clindamycin and teicoplanin against Clostridium difficile strains isolated from various origins in Poland was investigated. MIC was determined for metronidazole, clindamycin and teicoplanin. The disc-diffusion method was used for the vancomycin susceptibility test. Three out of thirty-eight strains were resistant to vancomycin and twenty-eight were susceptible to teicoplanin. A widespread MIC was observed for clindamycin and metronidazole. No correlation between the origin of the strain, toxigenicity and susceptibility to teicoplanin, clindamycin, vancomycin and metronidazole was evident.

Adolescent↗
PubMed1990

The effects of deoxycholate and sodium dodecyl sulphate on the serological reactivity of antigens isolated from six Bacteroides reference strains.

The detergents sodium dodecyl sulphate (SDS) and sodium deoxycholate (NaD) are frequently used as solvents for macromolecular polysaccharide complexes in immunochemical and serological techniques. The influence of the disaggregating surfactants on the serological reactivity of endotoxins isolated from six serotype specific reference strains of the Bacteroides fragilis group was investigated by comparing haemagglutinating and precipitating reactivities of antigen solutions in phosphate buffered saline (PBS), NaD and SDS. All antigens were phenol/water extracted endotoxins. Solutions of antigens isolated from serotypes A, B, C and D in PBS exhibited mainly serotype specificity and a few well known low-titer cross reactions; solutions in NaD showed additional cross reactivity, which was enhanced by solubilization of the antigens in SDS. In immunoelectrophoresis endotoxins isolated from serotypes A and C and dissolved in NaD or SDS showed additional precipitation lines compared to solutions of the same antigens in PBS. These changes in the serological reactivity are of relevance for investigations where the serological specificity of antigens is in question.

Antigens, Bacterial↗
PubMed1990

Immune response to endotoxin isolated from Bacteroides fragilis in the pregnant guinea pig.

The humoral immune response to endotoxin isolated from Bacteroides fragilis was analyzed in the pregnant guinea pig by means of passive hemagglutination, passive hemolysis, a modified Coombs test, and by crossed immunoelectrophoresis. Pregnant animals were immunized with endotoxin on day 30 of gestation, and antibodies were determined on day 61 in maternal and fetal sera, and in amniotic fluid. The IgG and IgM responses in maternal sera were of the same magnitude as in sera of nonpregnant animals. Fetal sera contained IgG and sometimes IgM, and a higher percentage of incomplete antibodies against endotoxin than maternal sera. Low-titer anti-endotoxin antibodies, partially sensitive to dithiothreitol, were found in amniotic fluid. A statistically significant reduction in the growth of fetuses from endotoxin-immunized females was observed.

Amniotic Fluid↗
PubMed1990

An extracellular material observed in Clostridium difficile strains.

Some extracellular material-- "capsule" has been observed among Clostridium difficile strains. C. difficile strains (44 examined) contain different proportions of cells with "capsule" and without "capsules". Toxigenic strains contained fewer capsulated cells than non-toxigenic strains.

Bacterial Proteins↗
PubMed1990

The toxicity of Bacteroides thetaiotaomicron cell-surface antigens to chicken embryos.

The toxicity of B. thetaiotaomicron lipopolysaccharides and capsular antigen to 11-day-old chicken embryos was examined. CELD50 (chicken embryo LD50) of these preparations were determined. All examined preparations showed the lethal activity for chicken embryos. The toxicity of lipopolysaccharides was much higher than the toxicity of capsular antigen.

Animals↗
PubMed1989

Detection of 2-keto-3-deoxyoctonate in endotoxins isolated from six reference strains of the Bacteroides fragilis group.

1. Endotoxins isolated from six serotype specific reference strains of the Bacteroides fragilis group were dephosphorylated by treatment with aqueous 50% hydrofluoric acid. 2. Mild acidic hydrolysis of the dephosphorylated endotoxins released 2-keto-3-deoxyaldonic acid, the presence of which was demonstrated by the colorimetric thiobarbituric acid assay (TBA). 3. Thin layer chromatography of the dephosphorylated lipopolysaccharide of B. fragilis IPL E 323 (serotype E2), after acidic hydrolysis, revealed a TBA-positive substance with the same Rf-value as authentical 2-keto-3-deoxyoctolusonic acid (KDO). 4. Quantification of 2-keto-3-deoxyoctonate-in the lipopolysaccharide of B. fragilis IPL E 323 by means of the TBA resulted in a KDO content of 15 nM mg-1 lipopolysaccharide.

Bacteroides fragilis↗
PubMed1989

Serological studies of Bacteroides vulgatus from normal gut flora.

A serological classification scheme for 48 strains of Bacteroides vulgatus was established by agglutination technique. Bacterial strains were isolated in our laboratory from gut flora of healthy persons. Absorbed antisera to 11 strains of B. vulgatus were prepared. All 48 strains tested reacted with one or more antisera. Among these strains, 22 serogroups were formed; 11 of them contained only one group component and 11 were made up of multiple group components.

Agglutination Tests↗
PubMed1989

The cell-surface components of Bacteroides thetaiotaomicron as coating antigens in ELISA test.

Cell-surface antigens were extracted out of three Bacteroides thetaiotaomicron strains of different origin. Lipopolysaccharides, their fractions, L1 preparations and capsular antigen were obtained. All substances were tested as coating antigens in ELISA test against antibacterial rabbit immune sera. The highest absorbances were observed with lipopolysaccharides (LPS), their polysaccharide fractions (PS) and capsular material (CPS). Lipopolysaccharides after nuclease treatment (N-LPS), free from nucleic acids, were more active than crude phenol-water extracts (PW-LPS).

Animals↗
PubMed1989

[Bacterial etiological factors of diarrhea in the stool of infants hospitalized in January 1987].

The results are presented of the second part of the studies conducted for monitoring of the variability of bacterial factors causing diarrhoea in the youngest children. The presently reported study comprised 42 babies aged from 7 days to 18 months treated in hospital in January 1987. The number of children with diarrhoea hospitalized in this period was relatively smaller than in January 1985, while the incidence of enteropathogenic bacteria in stools was very similar. They included mainly Klebsiella and enteropathogenic Escherichia coli strains, especially in the youngest children. In only one case Salmonella arizonae was found while in 1985 a very high number of Salmonella agona strains were cultured, especially from newborns. In both parts of the study Campylobacter jejuni and Yersinia enterocolitica were never found in stools. In both parts of the study the course of bacterial diarrhoea was mostly moderately severe, loose stools continued usually for 3-4 days, but in the second part more cases of severe diarrhoea were noted, which, however, could not have been related to the bacterial species cultured from stools or to the presence of mixed bacterial flora. The importance is stressed of the carrier state of enteropathogenic bacteria which was observed in three-fourths of patients in January 1987, while in January 1985 this was present in nearly all cases without diarrhoea.

Bacteria↗
PubMed1986

Comparison of three types of reagents for detection of Bacteroides fragilis by direct immunofluorescence.

Three different methods were used to prepare conjugates for the detection of rods of the Bacteroides fragilis group by direct immunofluorescence. Lyophilized conjugates were prepared. Three sets (five in each) of monovalent conjugates against serotype strains of B. fragilis (including conjugate E/E1 + E2) and polyvalent conjugate (A + B + C + D + E1 + E2) were obtained. Each conjugate was prepared in two variants: 1. unabsorbed, 2. absorbed with tissue powder prior to lyophilization. Conjugates obtained by precipitation of sera with 50% ethanol and direct coupling of gammaglobulins with stain were found to meet the requirement for good fluorescence reagents and are well suited for the detection of B. fragilis by direct immunofluorescence. Absorption of the conjugates with tissue powder before lyophilization did not affect their quality.

Ammonium Sulfate↗
PubMed1985

Immunochemical investigations of antigens isolated from Bacteroides ovatus strain ATCC 8483.

A saline extract (SE) and a phenol/water extract (WL) were prepared from Bacteroides ovatus strain ATCC 8483. A fraction CS was isolated from the culture supernatant. WL was further split by ultracentrifugation into lipopolysaccharide (LPS) and supernatant (L1). Fractions SE, WL, LPS and L1 reacted serologically with homologous antiserum but did not cross-react with antisera against heterologous Bacteroides serotypes. Fraction CS was inactive in haemagglutination, haemagglutination inhibition and immunoelectrophoresis tests. SE, WL, LPS and L1 proved to be serologically heterogeneous. A distinct serological specificity for SE was demonstrated. The serological reactivity in SE and WL was not altered after treatment with proteolytic enzymes yet completely destroyed in WL and partially in SE by sodium metaperiodate. SE, WL, LPS and L1 contained the sugar components rhamnose, fucose, ribose, mannose, galactose, glucose and glucosamine in different molar ratios for each fraction. Galactosamine was found in WL and LPS, uronic acid in WL and L1. Two unidentified aminohexoses were detected in WL, one of which was also detectable in L1 and SE. 2-Keto-3-deoxyaldonic acid was demonstrated in LPS and L1 after strong acid hydrolysis.

Antigens, Bacterial↗
PubMed1985

Properties of Bacteroides fragilis antigens with specificity B.

Five different serologically active preparations were extracted from Bacteroides ovatus ATCC 8483 strain. These substances and sixteen preparations of culture supernatants all giving a positive reaction with antiserum of serotype B were used as inhibitors in haemagglutination inhibition test. The results showed that substances obtained from the culture supernatants differ from endotoxin of Bacteroides ovatus ATCC 8483 serotype B.

Animals↗
PubMed1984

Immunochemical and biological studies of antigens isolated from a strain of Bacteroides fragilis.

Phenol/water-extracted lipopolysaccharide and a fraction of HM, extracted with acetate buffer pH 2.0, from Bacteroides fragilis strain 62/73 are antigenically different as shown by immunodiffusion, passive haemagglutination, haemagglutination inhibition and preliminary chemical investigations. Biological activity, assessed with the local Shwartzmann reaction, was demonstrated for the lipopolysaccharide whereas antigen HM was almost inactive in this test. HM is immunogenic in rabbits. Antibodies against HM were detected in seven out of ten sera of healthy humans.

Animals↗