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Biomedical subjects

F R Fliess

Publications and source records attributed to F R Fliess.

At least 19 recordsLinked to original sources
PubMed1990

[The effect of low-dose pre-ovulatory progesterone administration on the outcome of in-vitro fertilization (IVF) and intra-tubal gamete transfer (GIFT)].

The influence of application of low-dose progesterone 4 hours before induction of ovulation by HCG on the outcome of IVF and GIFT was investigated in a bilateral study. The number of patients was 38 from both clinics for IVF and 36 for GIFT. With a pregnancy rate of 35.3% and 17.6% of ongoing pregnancies per transfer the results of the study are much better than before. The rather low number of patients and the low rate of transfers relativate these results. After GIFT an elevated abortion rate leads to only 11.1% ongoing pregnancies following a pregnancy rate of 33.3%. The effect of progesterone supplementation on the LH-peak and the endometrium should be further investigated to find new ways to increase success of IVF and GIFT treatment.

Adult↗
PubMed1989

[Hormone profile and follicle development in ovarian stimulation treatment for in vitro fertilization. II. Prolactin, estradiol and progesterone profile].

144 patients were treated by 5 different stimulation protocols in the IVF-program of the University Women's Hospital Rostock (Clomiphene/hCG, Clomiphene/Anthrogon/hCG, Pergonal/hCG, Anthrogon/hCG, Folistiman/hCG). Estradiol-17 beta and progesterone were determined during the follicular phase of 172 cycles by RIA. The results were compared between the treatment groups by means of Student's t-test or Mann-Whitney's nonparametric test. Additionally, in 37 cycles prolactin was determined. The levels of estradiol-17 beta in all treated groups were higher as compared with spontaneous cycles. Between the groups statistically significant differences were found in start and velocity of E2-rise, in the average level of E2, and in the level of preovulatory peaks. The latent phase of stimulation was significantly longer in patients treated with pergonal than in Anthrogon treated patients. Moreover, in the Anthrogon group the rate of "high responder" was higher. On average, in patients with a very fast growth rate of estradiol-17 beta (greater than 1.9 nmol/l) during the late follicular phase the percentage of less mature oocytes was increased after follicular puncture. The levels of progesterone started to rise in all groups between day-2 and -1 (day 0: day of LH-peak). On day 0, patients with Pergonal and Anthrogon had the significantly highest level. Levels above the 1s-range occurred in 12 patients during the follicular phase. This elevation of progesterone took place in 3 patients without any rise of basal LH-levels. 80.4% of 37 patients investigated were found to have prolactin levels above 500 mE/l during the late follicular phase. This transient hyperprolactinemia correlated with the rise of estradiol levels and continued during the early and mid luteal phase. Hence preventive administration of bromocryptine appears to be advisable in cycles stimulated with gonadotropins.

Chorionic Gonadotropin↗
PubMed1989

[The hormonal micromilieu and oocyte status in the stimulated in vitro fertilization cycle].

FSH, LH, PRL, estradiol-17 beta and progesterone were determined in 651 follicular fluids of 173 patients treated by ovarian stimulation for IVF. The stimulation was performed according to 5 different schemes: clomiphene/HCG, HMG (Pergonal)/HCG, HPG (Anthrogon)/HCG, clomiphene/HPG/HCG, Folistiman (heterologeous pituitary gonadotropin)/HCG. The mean levels of hormones of all follicles of each stimulation scheme were determined and differences between the groups were estimated by Student's t-test and the x-square-test. Additionally, in a hierarchy of follicles made depending on the follicular fluid volume the hormonal levels were compared between different rank numbers of one stimulation group and between different groups of stimulation. The maturation of oocytes judged by a maturation index and their ability for cleavage in culture after insemination was investigated in relation to the hormonal content of the follicular fluid. Stimulation by gonadotropins (Pergonal, Anthrogon, Folistiman) led to an decreased mean level of follicular steroids. This was related to an increased part of follicles poor in steroids after stimulation by gonadotropins. Within the follicular population follicles stimulated by clomiphene/HCG had a reduction of the levels of estradiol in higher rank numbers, but there were no clear evidences for such a reduction in the other stimulated groups. In all stimulation groups a significant reduction of progesterone levels was observed in higher rank numbers of follicles. Oocytes with a high maturation index mainly derived from follicles rich in progesterone. After insemination, development of oocytes in culture was compatible even with very high or low levels of hormones. There was no relation of the levels in FSH and LH to cumulus expansion and levels of estradiol of the follicular fluid. There was also no clear correlation between the levels of prolactin in follicular fluid and the cleavage rate.

Estradiol↗
PubMed1989

[The effect of glass wool filtration on human spermatozoa--a comparison with the swim-up technic].

20 fresh human ejaculates (6X normozoospermia, 7X oligozoospermia and 7X asthenozoospermia) were prepared either with the swim-up-method as with the glass wool filtration. After both techniques the motility of the spermatozoa improved markedly. In cases of asthenozoospermia and oligozoospermia the motility increased significantly compared with the value of the ejaculate. From the motile spermatozoa of the ejaculate 45-60% were detectable in the filtrat after glass wool filtration or in the upper fluid after swim-up-procedure.

Female↗
PubMed1988

[Pregnancy following gamete intra-fallopian transfer (GIFT)].

In the present paper we will report on a pregnancy following a Gamete Intra Fallopian Transfer (GIFT). This is a 26 years old female, treated in our sterility dispensaire for a secondary sterility since 1982. All basic hormone levels were normal. The andrological examination of her husband showed an oligozoospermia stage 2. A following hormone treatment was unsuccessful. Stimulation, monitoring and laparoscopy are demonstrated.

Adult↗
PubMed1988

[Cryopreservation of early human embryo stages].

A short review of freezing procedures applied to early human embryos is given. It is noted that human embryos survived freezing and thawing at a developmental stage of 1. cell to blastocyst. But it seems to be necessary to use for any developmental stage of early embryo a special freezing and thawing method. Embryo survival is correlated with their morphologic features where as neither age of embryos nor developmental stage were involved in freezing and thawing ability.

Embryo Transfer↗
PubMed1987

[Hormone profile and follicle development in ovarian stimulation treatment for in vitro fertilization. 1. FSH and LH profiles].

144 patients were treated by 5 different stimulation protocols in the IVF-program of the University Women's Hospital Rostock (Clomiphene/HCG, Clomiphene/Anthrogon/HCG, Pergonal/HCG, Anthrogon/HCG, Folistiman/HCG). In 172 cycles FSH and LH were measured in serum during the follicular phase by RIA. The results were compared between the treatment groups and a group of normal women by Student's t-test or Mann-Whitney's nonparametric test. Moreover, in each group the number of follicles visible by laparoscopy and number of punctured follicles were estimated. The maturation of collected oocytes were judged by a maturation index. Modus of action and FSH/LH-ratio of used drugs have been shown to influence serum levels of FSH. Every stimulation protocol was characterized by typical profiles of hormones. Especially, immunoreactive levels of FSH were related to development of follicles. Different biological action of various stimulation regimes was demonstrated by a shortage of follicular phase from 13,4 days in the Clomiphene group to 11,0 days in the Folistiman group, an increase of laparoscopically visible follicles from 4,0 to 13,2 and a rise of collected oocytes from 1,6 to 7,3 per laparoscopy. Quality of oocytes has been shown to be related significantly to the modus of stimulation. Anthrogon-protocol was the most effective stimulation regime according to profile of gonadotropins, the number of follicles and the structure of harvested population of oocytes. However, even in this group co-dominant development of follicle was not achieved. Application of pure FSH and an earlier start of stimulation treatment are possibilities for improvements of results in IVF by stimulation.

Chorionic Gonadotropin↗
PubMed1987

[Dynamics of follicle development in stimulated in vitro fertilization cycles--sonographic follow-up and findings following follicle puncture].

Results of ultrasonical folliculometry during treatment by 5 different stimulation protocols were evaluated in 122 cycles of IVF-patients. Additionally, the structure of follicular population was investigated by measurement of follicular volume which was the basis of a follicular hierarchy. - Number of developing, sonographically detectable and punctured follicles increased significantly from stimulation by Clomiphene to stimulation by Folistiman. Only in stimulation by Clomiphene number of sonographical visible follicles was in accordance with laparoscopically punctured follicles. Development of relationship of the follicular classes during treatment supports hypothesis that exogenous gonadotropins do not increase follicular recruitment in the follicular phase but prevent atresia of follicles recruited earlier. Long lasting action of FSH as in stimulation by gonadotropins stimulates a greater number of follicles more intensively as demonstrated by an increased follicular volume in follicles with high rank numbers in gonadotropin treated patients compared with treatment by Clomiphene and Clomiphene/Anthrogon. Very high FSH-levels probably cause a heterogenous population of follicles with an increased number of small follicles at time of follicular puncture.

Chorionic Gonadotropin↗
PubMed1984

[Comparative light- and electron microscopy findings in oocytes obtained by laparoscopy during the cycle period close to ovulation].

Oocytes recovered by diagnostic laparoscopy can be divided in three categories--atretic, non-ovulatory and pre-ovulatory oocytes. Examples for those three categories are demonstrated. The findings from survey sections and from transmission electron microscopy were used for the retrospective characterization of the light micrographs and the description of the surroundings. Methodic experiences allow further research on oocytes and embryos with developmental defects. Two examples were shown. Our experiments yielded, that the examination with light and electron microscopy is suitable for statement in diagnostic laparoscopies and that these examinations were advantageous for the preparation of an in vitro fertilization program.

Female↗
PubMed1984

[In vitro fertilization program in therapy of the sterile marriage. International status and personal experiences].

A review is given about in vitro fertilization (IVF/ET) and embryo transfer in the treatment of sterile couples. Programmes of in vitro fertilization of successful international groups are analysed and discussed, especially the improvements of the method. These international reports were the basis of the programme of in vitro fertilization at women's hospital of the Wilhelm Pieck University at Rostock. The organization of the programme depends on local conditions. Treatment is performed in some so-called IVF-series. The results of the first and second series with 52 patients are reported. The only pregnancy during the second series was an ectopic pregnancy.

Adult↗
PubMed1984

[In vitro fertilization and embryo transfer. Personal experiences in the introduction of laboratory methodology].

Our laboratory technique includes recovery and handling with gametes outside the body, in vitro fertilization, culture of embryos, and finally embryo-transfer. Time-table, culture media and technique of the cultivation as well as laboratory devices are described and than compared with informations about methods from other teams. The necessity of transport of the follicle fluids from operating theatre to laboratory is given caused by local conditions. All dates about number of oocytes (73), quantities of preovulatory oocytes (47) and cleavage rates (55%) are descended from 30 laparoscopic oocyte recoveries of the 2. in vitro fertilization program of the university woman hospital in Rostock, January and February 1984.

Culture Media↗
PubMed1984

[A transportation vehicle for laparoscopically obtained follicular specimens].

Structure, function and first results with a vehicle for transportation were described. This apparatus allows to store laparoscopic harvested follicular fluids for a while in constant temperature and in air condition with 5% CO2 in compressed air. Simultaneously the follicular fluids were transported in this vehicle from operating theatre to the laboratory.

Female↗
PubMed1984

[First experience with an in vitro fertilization program at the Rostock University Clinic of Gynecology and Obstetrics].

In vitro fertilization as an aid in diagnosis and treatment of the sterile couple has been used at the university clinic of gynecology and obstetrics in Rostock since the beginning of 1983. 17 women were treated in the first series of in vitro-fertilization and embryo transfer in September and October 1983. After treatment with clomiphene and HCG in 16 women one or more oocytes were harvested. In 14 women one or more oocytes were fertilized. Embryo transfer was performed in five cases. No pregnancy resulted. The causes of the low cleavage rate and possibilities for improvement of the results are discussed.

Clomiphene↗
PubMed1984

[Problems of ethics in research and practice in reproduction medicine].

The methods in management of infertility with manipulating of gametes require new ethical positions. In GDR artificial donor insemination, in vitro fertilisation and embryo transfer are ethically accepted because they are important possibilities for accomplishment of life in a childless partnership. The problems with egg and embryo donation, surrogate mother and other new methods are discussed.

Embryo Transfer↗
PubMed1984

[Recent findings on follicle and oocyte maturation. 1. Development of follicles and maturation of follicles].

A review is given of the present knowledge of the development of follicles and oocytes, especially in the human. The first report deals with the course of development of follicles from the beginning in the fetal ovary to the mature Graafian follicle in the adult women. Questions of terminology, functional morphology and important aspects of the regulation of follicular growth are discussed. The last part of the paper summarizes the present day possibilities in monitoring growth and maturation of ovarian follicles in clinical practice.

Androstenedione↗
PubMed1984

[Recent knowledge on follicle and oocyte maturation. 2. Oocyte development and maturation].

A review is given about the present knowledge in oocyte development and oocyte maturation. The four parts of the review contain: development of the oocyte in the fetal ovary, morphology and metabolism during meiotic arrest, oocyte maturation, and the relations between oocyte maturation and in vitro-fertilization in the human. The morphological and biochemical changes in the maturation process and present hypotheses about maturation regulation are described especially. The increasing knowledge in this field supports the progress of in vitro-fertilization in the human. On the other hand this technique contributes importantly to new directions in oocyte research.

Cell Differentiation↗