Biomedical subjects

F R Fliess

Publications and source records attributed to F R Fliess.

21 records · Page 2Linked to original sources

PubMed1981

[Orientation studies of ovulation release in mice to test gonadotropic preparations. 2. FSH, PMSG and Gn-RH activity].

The doses of FSH (follicle-stimulating hormone), PMSG (pregnant mare serum gonadotrophin), and gn-RH (gonadotrophin-releasing hormone) effective in terms of triggering ovulation were determined in a mouse ovulation test. Varying doses of the above preparations were subcutaneously injected, 48 hours after overstimulation by injection of 0.5 or 1.0 IU of PMSG. The animals were sacrificed for examination, after another 18-20 hours had passed. Roughly 50 per cent of all animals treated (threshold) in one and the same dosage group (n = 5) had ovulated in response to 0.02-0.1 IU of FSH per animal. The following FSH and PMSG dosages are recommended: 0.02, 0.04, 0.06, 0.08, and 0.1 IU of FSH, 0.6, 1.0, 1.4, 1.8, 2.2, 2.6, 3.0 IU of PMSG. When mouse ovulation tests were used in orientation studies, ovulation was regularly induced by Gn-RH doses per animal between 0.01 and 1.0 micrograms. Dosage spacings or increments should be specified with higher accuracy by further studies.

Animals↗

PubMed1981

[Orientation studies of ovulation release in mice to test gonadotropic preparations. 1. Dosage of PMSG and HCG].

Reported in this paper are the authors' own experience and results concerning a method of testing ovulation in mice by which activities of gonadotrophic preparations can be determined. Prepuberal female mice received as pretreatment a subcutaneous injection of 0.5 or 1.0 IU of PMSG (pregnant mare serum gonadotrophin) per animal. When HCG (human chorionic gonadotrophin) ws subcutaneously injected, 48 hours later, ovulation was induced in all animals and could be microscopically counted between two glass slides under slight pressure, after another 18 hours had elapsed. The highest effective dose (ED100) in most of 40 comparisons was close to 1.0 IU of HCG. Dosages of 0.2, 0.4, 0.6, 0.8, and 1.0 IU of HCG were found to be suitable for effective dosage determination. Differences in action between various batches of lyophilised HCG were elucidated by using two variables, a threshold reached when three of five animals in one and the same dosage group had ovulated, and ED100, when all had ovulated.

Animals↗

PubMed1980

[Trials to eliminate the sex odor of growing boars through active immunization with an androstenone-protein conjugate].

Determination of immunogenicity in rabbit was followed by the use of 5 alpha-Androst-16-en-3-on cattle serum albumin conjugate (AC-) in Freund's adjuvant for active immunisation of young growing boars against boar pheromone. Two experiments were undertaken. Five boars, aged 140 days, were involved in the first experiment. They received six AC- doses in intervals of 14 days (4 mg, 5 mg, 6 mg, 7 mg, 8 mg, 9 mg). Each of the doses was applied to four points, two of them subcutaneous and two intramuscular. In the second experiment, four boars, aged 121 days, received 40 mg AC- each in eight applications, among them three injections in intervals of one week, followed by a pause of two weeks, another three injections again in intervals of one week, and two injections in intervals of four weeks. Another group of four animals received 20 mg AC- in four applications in intervals of four weeks. Five control boars were included in the first experiment and four in the second. Immune response of the experimental animals was detected neither by immunoelectrophoresis nor by radio-immuno-assay. Pheromones in fat samples were determined by means of gas chromatography during treatment and after slaughter. They were increased in some of the cases, albeit not significantly. The nuclear diameters of the interstitional cells of Leydig in testicular sections differed but little, with no significance being recorded.

Adipose Tissue↗