Biomedical subjects

F Rank

Publications and source records attributed to F Rank.

64 records · Page 4Linked to original sources

PubMed1978

The healing process of transplanted digital tendon sheath synovium. Experimental autoradiographic studies in chickens.

The present investigation is a sequel to a previous report on the composition and fate of the pseudo-sheath formed by secondary healing, and of free tendon sheath autografts covering defects in the digital tendon sheaths of chickens (Eiken & Rank, 1977). Autoradiography was used to determine graft survival and cell regeneration. The distribution of labelled cells indicated that the granulation tissue of secondary healed defects formed around implanted Silastic rods consists of fibrous scar tissue. The tendon sheath autografts, on the other hand, were found to survive, demonstrating a well-preserved synovial surface. These findings probably imply new concepts of tendon surgery. The Silastic rod induced some foreign body reaction in the healing synovium. It is assumed that this reaction is reversible, but this could not be established in the present investigation. Further study is being made of this problem.

Animals↗

PubMed1978

Oesophageal tissue reaction to different suture materials. An experimental study in the cat.

Oesophageal tissue reaction to different suture materials (chromic catgut, silk, prolene and stainless steel wire) was analysed and compared in 45 cats with and without reflux oesophagitis. Diffuse reflux oesophagitis was produced in 12 out of 21 animals subjected to oesophagocardiomyotomy (Marwedel-Wendel) combined with production of a fixed hiatal hernia of sliding type. Steel wire sutures produced minimal tissue reaction and undisturbed fibroplasia both in the intact oesophagus and in the presence of reflux oesophagitis. The steel wire sutures were found to be superior to the chromic catgut, silk, and prolene.

Animals↗

PubMed1977

Experimental restoration of the digital synovial sheath.

The digital synovial sheath constitutes an important component of the delicate mechanism of flexor tendon nutrition and gliding function, In the present study the true nature of the inner cell layers of secondary healed defects in the tendon sheath as well as of free tendon sheath autografts were studied. Leghorn chickens were used as experimental animals and the gradual development of the pseudosheath as well as the healing of sheath autografts were studied both macroscopically and histologically including transmission electron miscroscopy. Synovial regeneration by extension from intact parts of the sheath was never observed and the pseudosheath formed around silastic rods consisted of granulation tissue with fibroblasts and macrophages. The free tendon sheath autografts demonstrated a normal process of healing at the edges of the defect. Synovial regeneration appeared to be that of metaplasia and proliferation of fibroblasts and macrophages. This phenomenon was demonstrable both in the secondary healed defects and more convincingly in the sheath autografts. Further, the silastic rod was found to induce foreign body reaction in the healing synovium. It is concluded that grafting of autologous tendon sheath tissue seems to be a promising method for restoration of defects in the digital tendon sheath.

Animals↗

PubMed1977

Synovium as a nutritional medium in tendon grafting.

The mechanism of tendon healing and the role played by the synovial sheath are still obscure and controversial. In the present experimental study free tendon grafts were nourished by synovial fluid only. By morphological and cytochemical techniques viable cells were found in the superficial zones of the tendon, capable of both proliferation and production and production of new collagen. This repair process affected predominantly the ends of the grafts. At the centre of the specimens degenerative changes appeared to an increasing extent throughout the observation period of 12 weeks. Adhesions to the surrounding tissues did not form. It is concluded that fibroblasts, most probably originated from the superficial cell layers of the tendon are capable of regeneration and synthesis of new collagen and that synovial fluid plays an important role in the metabolic exchange of the superficial scantily vascularized areas of the tendon. The observations are discussed in relation to the present concepts of tendon surgery.

Acid Phosphatase↗

PubMed1976

Studies on healing of Debrisan-treated wounds.

Debrisan (Pharmacia AB, Sweden) is a highly hydrophilic dextran polymer in the form of dry, spheric beads. When dry Debrisan beads are placed on a wound, the wound secretions are sucked up into and between the beads. No crust is formed on the wound. Bacteria granular substances are removed with the secretions. Treatment with Debrisan was found to be very effective in cleansing discharging wounds. Since Debrisan treatment removes substances from the wound area, e.g. prostaglandins, this might affect the process of repair. The morphology of wounds was studied with the aid of histological and histochemical methods. In 15 patients, donor sites on one thigh, treated with Debrisan, were compared with donor sites on the contralateral thigh, subjected to open treatment. In pigs, comparison was made between Debrisan-treated with Vaseline-gauze-covered dermal burns. In man, the inflammatory reaction was less pronounced in the Debrisan-treated wound and located more superficially then in the wound left open, where perivascular inflammatory cell infiltrates were found even deep in the dermis. In pigs the burns exhibited similar differences. Thus the inflammatory reaction in the Debrisan-treated wounds was less pronounced than in the control wounds. Debrisan beads were never seen below the wound surface. No unfavourable influence of Debrisan treatment was observed on the process of repair.

Animals↗

PubMed1975

Histological studies on granulation tissue from bipedicle tube flaps.

Histological studies were performed on granulation tissue from cylinders implanted 2 to 8 weeks and 4 months in bipedicle tube flaps on rabbits. Tissue from cylinders implanted in skin folds were used as controls. Flap and control tissue showed similar changes over the weeks. At weeks 2 and 3 the tissue was rich in cells, blood vessels and collagenous fibres. At week 4 the tissue showed transitional changes to week 5, when there were zones of loosely textured tissue with fine, fragmented collagen fibres. At week 5 some of the loose tissue was replaced by fat. Macrophages and inflammatory cells had appeared. By week 7 the fatty and collagenous tissue was mature and macrophages and inflammatory cells had disappeared.

Adipose Tissue↗

PubMed1975

The role of the digital synovial sheath in tendon grafting. An experimental and clinical study on autologous tendon grafting in the digit.

The mechanism of healing of a free tendon graft and also the role played by the synovial sheath is still obscure and debated. In the present study an effort is made to further elucidate these problems. In rabbits and dogs an experimental model was developed where a free tendon graft would be nourished by synovial fluid only. Macroscopically the grafts remained smooth, white and glistening without vascular ingrowth or adhesions during an observation period of 12 weeks. By histological and histochemical techniques the grafts as a whole were found viable up to 3 weeks. In a clinical series of 40 free flexor tendon grafts within the digital area as much as possible of the tendon sheath was preserved during grafting. The functional recovery was generally very good. The best results were obtained in those cases where most of the tendon sheath was preserved during grafting. The functional recovery was generally very good. The best results were obtained in those cases where most of the tendon sheath was preserved. No signs of defective graft survival were observed. Based on both experimental and clinical results from the present study it is concluded that there is no reason to excise the remaining tendon sheath within the critical zone in the digits. On the contrary preservation of the sheath contributes to survival of the graft and helps maintaining the integrity of gliding surfaces with maximal functional restoration and minimal formation of adhesions.

Acid Phosphatase↗

PubMed1975

Granulation tissue formation in viscose cellulose sponges of different design.

Granulation tissue formation was studied in subcutaneously implanted viscose cellulose sponges of different design using histological and chemical methods. Two different models of sponges were used, one of cubical shape and another rectangular and thin. The sponges had the same weight. After one week of implantation, histological examination of the cubical sponges showed granulation tissue only in the periphery and a central cavity filled with inflammatory cells. The histological texture of the thin sponges showed that they were almost completely filled with granulation tissue with a minimal central cavity and very few inflammatory cells. DNA values of the cubical sponges were about 20% higher than those of the thin sponges. After aspiration of the exudate in the cubical sponges, the DNA values were about the same as those in the thin sponges. The amount of hydroxyproline and RNA were about the same regardless of whether the sponges were cubic or thin or the exudate of the cubic sponges had been aspirated or not. At two weeks there was still a central cavity in the cubic sponges but very few inflammatory cells. DNA values at this time of observation were also the same in both models of sponges. Thus the thin-shaped model of sponge seems to be preferable to the cubic one when the time of observation is shorter than two weeks.

Animals↗

PubMed1975

Granulation tissue formation in oophorectomized rats treated with female sex hormones. I. A histological study.

Granulation tissue formation was studied in experimental granulomas up to three weeks in oophorectomized rats treated with female sex hormones. The influence of estrogen and progesterone during the early phase of healing could sucessfully be followed histologically. The amount of DNA and hemoglobin in the granulomas correlated well with the amount of cells and the capillary bed found in the histological sections.

Animals↗

PubMedDate not supplied

[Mitotic regimen of histologic variants infiltrating duct carcinoma of the breast].

Mitotic regimen was studied in 197 cases of infiltrating duct mammary carcinoma found at mammography screening and subdivided according to the prognostic F. Linell classification (1980) into two groups (1-tubular, duct-tubular and II-comedo-type carcinoma). Indices of the mitotic regimen and particularly mitotic index showed statistical differences in these groups. Comedo-type carcinoma with a poorest prognosis is characterized by a high mitotic index (near 20% and higher) and pronounced mitosis pathology correlated with a degree of cell polymorphism. Mitotic index of a tubular and duct-tubular carcinoma is much lower (as a rule lower than 10%) this reflecting their more favourable prognosis.

Adult↗