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Biomedical subjects

F Rousselet

Publications and source records attributed to F Rousselet.

At least 37 records · Page 2Linked to original sources
PubMed1989

An alpha slow-moving high-density-lipoprotein subfraction in serum of a patient with radiation enteritis and peritoneal carcinosis.

An alpha slow-moving high-density-lipoprotein (HDL) subfraction was seen in a patient presenting with radiation enteritis and peritoneal carcinosis, who was given long-term cyclic parenteral nutrition. This subfraction, observed in addition to normal HDL, was precipitated with low-density lipoproteins (LDL) and very-low-density lipoproteins (VLDL) by sodium phosphotungstate-magnesium chloride. The patient's serum lipoproteins were analyzed after fractionation by density gradient ultracentrifugation. The alpha slow-moving HDL floated in the ultracentrifugation subfractions with densities ranging from 1.028 to 1.084 kg/L, and their main apolipoproteins included apolipoprotein E in addition to apolipoprotein A-I. These HDL were larger than HDL2. The pathogenesis of this unusual HDL subfraction is hypothesized.

Apolipoprotein A-I↗
PubMed1988

[Comparison of protein concentrations in saliva and serum].

Saliva and serum protein profiles composed of 12 proteins: IgA, IgG, IgM, albumin, transferrin, alpha 1-glycoprotein acid, alpha 2-macroglobulin, alpha 1-proteinase inhibitor, haptoglobine, C3C, C4 and CRP were obtained by laser nephelometry of aliquots of samples of non-stimulated whole saliva and blood taken at the same time. The population of 138 controls of both sexes was divided into 4 age groups: 1 (20 to 29 years), 2 (30 to 39 years), 3 (40 to 49 years), 4 (50 to 60 years). Using the techniques developed for saliva assay, 10 proteins were measurable: IgA, IgG, IgM, albumin, transferrin, alpha 1-glycoprotein acid, alpha 2-macroglobulin, alpha 1-proteinase inhibitor, haptoglobulin and C3C. The C4 fraction and CRP were present at concentrations lower than the detection limit i.e. at 1.8 and 2.05 mg/l. In the serum 11 proteins were analysed, CRP occurring at less than 0.01 g/l. The statistical two-factor variance analysis test (sex and age) demonstrated the statistically significant influence of age in the saliva for alpha 1-PI and alpha 2-M in the serum for Tf, alpha 2-M and alpha 1-PI, and sex in the serum for IgG, IgM, alpha 1-PI, alpha 2-M and also the interaction of both factors in the serum for alpha 2-M.

Adult↗
PubMed1988

Rapid evolution in the immunochemical findings of a gamma heavy chain disease.

We noted the following major immunochemical changes in the final course of a gamma heavy-chain disease associated with a myelomonocytic leukemia: an important oligoclonal increase of IgG1/kappa, during sepsis development, whereas the lambda molecules decreased and IgG2 and IgG3 completely disappeared in a one-month interval. Initial concentrations of IgG4 were stable during that period, and gamma 1 heavy chains were slightly increased. This is a very unusual observation of the successive immunochemical events terminating a gamma heavy-chain disease.

Aged↗
PubMed1988

Analytical problems encountered in determining aluminum status from hair in controls and hemodialyzed patients.

The problems involved in evaluating aluminum concentrations in hair are reviewed, especially those concerning removal of contaminating metals, a critical factor. In the few published studies of Al concentrations in hair, acetone was usually used for its removal. Here, its use in the washing sequence was found to give less precise results and higher Al values than the use of isopropanol. With isopropanol, the whole analysis can be done in a single tube. We compared results with those in the literature. We found that the Al concentration in the hair of control subjects was not related to sex or hair color and that there was a highly significant (P less than 0.001) difference between values for control subjects and hemodialyzed patients: 126 (SD 58) nmol/g, n = 49, vs 226 (SD 104) nmol/g, n = 39, respectively.

1-Propanol↗
PubMed1987

Se, Zn, Mg, Ca, K, cholesterol, and creatine kinase concentrations in men during the 12 days after an acute myocardial infarction.

We determined concentrations of selenium, zinc, magnesium, calcium, potassium, total and high-density lipoprotein cholesterol, and total creatine kinase (EC 2.7.3.2) in plasma (P) and (or) erythrocytes (E) of 32 men at the time of hospital admission for acute myocardial infarction and then on subsequent days 2, 3, 6, 10, and 12. During these 12 days, only P-selenium and high-density lipoprotein cholesterol concentrations remained unchanged. For the other variables, individual comparison of means indicated the dates of significant changes in concentration (p less than 0.05).

Adult↗
PubMed1986

[Contribution of Jacques Canal in the field of lipoproteins].

The scientific works of J. Canal have essentially related to low density lipoproteins (L.D.L.), between 1.025 and 1.050. By using proteolytic enzymes and phospholipases, he is able to obtain informations on the accessibility of apolipoprotein B and phospholipids. So, as early as 1975, he expresses the hypothesis that only 30 p. cent of Apo B occupy a superficial position. In addition, he proposes the fact that Apo B has at least four antigenic determinants; this hypothesis formulated by J. Canal, almost 10 years ago, was recently confirmed by using monoclonal antibodies. At the same time, using phospholipases, J. Canal reaches the conclusion that phospholipids are localized on the surface of L.D.L. He demonstrates that phospholipids play a major role in the precipitation of L.D.L. by sulfate polysaccharides in the presence of L.D.L. by sulfated polysaccharides in the presence of divalent cations (Reaction of Burnstein). He proposes a mechanism for this reaction. This research on the mechanism of Burnstein's reaction will receive in clinical biochemistry an important application with the development of a completely original technique of dosage of total lipids in the serum.

France↗
PubMed1986

Isolation and characterization of an abnormal alpha slow-moving high-density lipoprotein subfraction in serum from children with long-standing cholestasis.

An abnormal high-density lipoprotein (HDL) subfraction, detected during periods of mild jaundice in the serum of seven children with chronic cholestasis from birth, was isolated and characterized. This fraction, identified by its slow alpha electrophoretic migration, is present in addition to normal HDL and differs from the abnormal HDL previously described in cholestatic syndromes. It is devoid of apolipoprotein B but is precipitated by phosphotungstate-MgCl2. These properties allowed its isolation by double selective precipitation. This subfraction is undetectable with this procedure in the serum of healthy subjects, is rich in cholesterol, and contains a large amount of apolipoprotein E, which may explain its precipitation by phosphotungstate-MgCl2. These apo E-containing HDL may play a major role in the lipid metabolism of patients with long-standing cholestasis during periods of mild jaundice.

Adolescent↗
PubMed1984

Six approaches to routine quantification of human monoclonal immunoglobulin M compared.

Six routine techniques for the determination of monoclonal IgM are compared: standard electrophoresis, the Technicon AIP system, Hyland laser nephelometry, Beckman Immunochemistry System, Behring laser nephelometry, and radial immunodiffusion. The data are evaluated by use of a nonparametric statistical analysis of variance by ranks (Friedman test) and Wilcoxon's test. The 27 sera containing monoclonal IgM that we studied yielded different results by the various techniques. Sera from a "reference" population of 15 subjects having above-normal polyclonal IgM yielded identical results for all techniques. These statistical findings agree with the biophysical mechanisms involved in the various ways of quantifying IgM. We conclude that these different systems are equivalent for estimating polyclonal IgM, but cannot (and should not) yield identical values when used in monoclonal IgM evaluations. Results for monoclonal IgM must be interpreted in terms of the molecular forms and immunological specificities of the assayed IgM.

Antibodies, Monoclonal↗
PubMed1983

[Lipoprotein (a)].

Lp(a) or pre-beta 1-lipoprotein or Sinking pre-beta LP (SPB) is a special LP. Its very high density (1050 to 1120) means that it can be extracted from LDL and HDL. In common with the LDL, it possesses apo B and a similar size. It is characterized by the presence of specific apoproteins, non-immunoreactive albumin and a carbohydrate chain rich in sialic acid. Its transmission is hereditary; there is a major locus transmitted by autosomal dominant inheritance. Another hypothesis suggests that it is transmitted autosomally according to a polygenic mechanism for 75 p. cent of the molecule with superimposition of non-genetic elements for the rest. Lp(a) is not a product of Lp(b) metabolism; it is not transformed into any other LP and it does not exchange its apoproteins. Its synthesis is constant over time and its function is not yet known. Its assay depends on the antigenicity of the specific determinants of the apo Lp(a). Only radio-immunoassay is able to give values for any sample size especially for levels of less than 50 to 80 mg/l (respective limits of the EID and IDR techniques). It appears that a high Lp(a) level could constitute an additional risk to developing coronary heart disease and the limit beyond which this risk exists could be 300 mg/l in normolipidaemic subjects. The in vitro binding of LP to glycoaminoglycanes (GAG) could be a model for studying LP binding in vivo. It has been seen that, by the intermediary of a low concentration of Ca++ ions, the reactivity of Lp(a) to GAG was selective.

Blood Glucose↗
PubMed1983

[Assay of cystine aminopeptidase activity on a centrifuge analyser].

The assay of the serum cystine-aminopeptidase activity, of placental origin, is a useful test for monitoring pregnancy. The authors present an adaptation of Van Oudheusen's method on a centrifuge analyser which allows a rapid kinetic measurement of this activity and they justify their choice of procedure. The values obtained in non-pregnant women and in pregnancies of between 8 and 40 weeks of amenorrhoea are presented.

Adolescent↗
PubMed1983

[Assay of serum selenium by atomic absorption spectrophotometry. Value in the biological diagnosis of nonobstructive cardiomyopathies].

The authors present a technique of direct assay of serum selenium by atomic absorption spectrophotometry, using electrothermal atomization in a graphite oven. The initial results of the assays performed in patients with non-obstructive cardiomyopathies (NOCM) reveal a decrease in the serum selenium level. This element could therefore represent a laboratory marker for these diseases.

Cardiomyopathies↗