PubMed Health⌕ Search

Biomedical subjects

F Rousselet

Publications and source records attributed to F Rousselet.

At least 55 records · Page 3Linked to original sources
PubMed1983

[Determination of erythrocyte sorbitol].

An increase in the intra-cellular concentrations of sorbitol can be responsible, at least in part, for certain long term complications of diabetes. Since the erythrocyte concentration of this polyol is a good indicator of that of other cells, we propose a simple, rapid enzymatic assay technique for red blood cells. The results already obtained reveal a significant difference between the erythrocyte sorbitol concentration in non-diabetic subjects and that in diabetic patients.

Adult↗
PubMed1982

[Serum lipoproteins in chronic alcoholics. Changes in HDL, VLDL and LDL as a function of the degree of hepatic involvement].

Changes in lipoprotein fractions HDL and LDL-VLDL were investigated in 84 chronic alcoholic patients and attempts were made to correlate these changes with the severity of liver damage. In 40 patients with undetectable hepatic lesions the lipoprotein fractions were not reduced but a rapidly reversible increase in HDL was noted in 15%. The 14 patients with steatosis and alcoholic hepatitis showed a fall in HDL, often associated with an increase in plasma triglycerides; these changes regressed during weaning. The 30 patients with cirrhosis of the liver had irreversibly low HDL and LDL levels.

Adult↗
PubMed1981

[Determination of the serum concentration of lipoproptein x (author's transl)].

Lipoprotein X (LPX) is a cholestase marker. The authors wish to set forth a simple and quick method for the quantity determination of this abnormal--a method which could be combined with other biochemical tests permitting the demonstration and monitoring of a cholestatic syndrome. The phospholipid composition of lipoprotein X is thought to be constant; therefore, the proposed method of quantity determination consists in an enzymatic quantity determination of phospholipids, performed on the lipoprotein, isolated from the normal serum lipoproteins by means of a simple operative protocol: elimination of the LDL and VLDL by means of immuno-precipitation, followed by separation of the HDL by means of the action of phosphotungstate. In addition to its quantitative characteristic, the proposed method has been shown to be more selective than the traditional demonstration which uses electrophoresis on agar. This electrophoretic research can in effect, show itself to be negative if practised on biological samples which are rich in biliary acids or free fatty acids; conversely, the operative protocol described permits the demonstration of the quantification of lipoprotein X.

Biliary Tract Diseases↗
PubMed1979

[Influence of phosphatidylcholine/cholesterol molar ratios in liposomes on cholesterol reactivity with cholesterol:oxygen oxidoreductase].

The reactivity of sonicated phosphatidylcholine-cholesterol liposomes with cholesterol : oxygene oxydoreductase, an enzyme which catalyses the oxidation of the 3 beta hydroxyl group of cholesterol to a ketone group, is compared with that of ternary system phosphatidylcholine-cholesterol-Thesit. Regardless to the phosphatidylcholines nature and the phosphatidylcholine/cholesterol molar ratio (R), the enzymatic oxidation rate of liposomal cholesterol is slower than when the reaction is developed in the present of Thesit, a surfactif agent which destroyes the lamellar particles. This is true whether Thesit is added during preparation of dispersions or during incubation with cholesterol oxydase. The enzymatic oxydation rate of cholesterol of ternary systems phosphatidylcholine-cholesterol-Thesit is independent of the (R) value and the phosphatidylcholine fatty acid unsaturation, whereas that of phosphatidylcholine-cholesterol dispersions depends on these two parameters. The reaction rate increases in the order: dipalmitoylphosphatidylcholine to yolk egg phosphatidylcholines, and dioleylphosphatidylcholine. The optimal conditions for cholesterol oxidation were found to be R = 0.5. This result is not affected by the phosphatidylcholines nature. In order to explain these data, various hypotheses are considered. In particular, the weak liposomal cholesterol reactivity with cholesterol oxidase could result from an inhibitory effect on the enzyme-substrate combination due to the polar phosphorylcholine groups.

3-Hydroxysteroid Dehydrogenases↗
PubMed1979

Action of tiadenol and clofibrate on biliary excretions of cholesterol and bile salts in rats.

Biliary excretion of cholesterol, phospholipids and bile salts, and the excretion of labeled cholesterol injected intravenously were studied in rats treated for 1 or 3 weeks with the hypolipidemic drugs tiadenol and clofibrate. Total biliary cholesterol concentration and output decreased in treated rats, and the cholesterol solubility ratio increased. The excretion of labeled bile salts increased with both drugs; the excretion of labeled cholesterol increased with clofibrate and tended to decrease with tiadenol. The ratio of labeled bile salts/labeled cholesterol was higher with tiadenol than with clofibrate.

Animals↗
PubMed1979

[Comparison by isoelectrofocusing between blood immunoglobulin G of the mother and her newborn. Value of the method (author's transl)].

The present investigation deals with the study about immunoglobulins in blood of twenty six couples mother-newborn. The immunoglobulins G were determinated by radial immunodiffusion and we stated the profile of serum's proteins after isoelectrofocusing. The results obtained from this study, showed a really important percentage of very differents profiles between mother and newborn's proteins. These divergences are independently from the level of immunoglobulins. The purpose of this investigation is to show the very high analytical power of the method. But it is limited in interpretation of results. With conventional protein procedures, it is not possible to say if the newborn is able to synthetize immunoglobulins very early in life. On the contrary, with specific methods of revelation, isoelectrofocusing seems the better procedure of investigation for identity immunoglobulins in blood.

Coloring Agents↗
PubMed1977

[Effect of cortisone on calcium metabolism. Attempt at correction by 5,6 trans 25-hydroxycholecalciferol, 25-hydroxycholecalciferol and vitamin D2].

14 patients receiving small doses of predisolone and 7 high doses were divided at random into three groups. Each group received for four weeks, 100 microgram of vitamin D2 or 25 hydroxyvitamin D3 or 5,6 trans-25 hydroxyvitamin D3. The 25 hydroxy and 5,6 trans-25 hydroxyvitamine D3 were able to increase intestinal calcium absorption. 25 Hydroxyvitamin D3 had an osteolytic action, increasing urine calcium and hydroxyproline and, once, in a patient slight hypercalcemia was observed. By constrast, 5,6 trans-25 hydroxyvitamin D3 decreased PTH and caused a significant decrease in urine hydroxyproline.

Calcium↗
PubMed1977

[Initial trial of a new test for the evaluation of hepatocellular function: the TMB test].

The oral administration of 400 mg of trimethoxy 1-3-5-benzene or T.M.B. is normally followed by a high urinary excretion of dimethoxy, 1, 3, hydroxy 5 benzene and other metabolites mainly in the form of conjugates. Urinary excretion during the first 3 hours is strongly reduced in liver disease. By a simple blind test we showed that the test is positive in subclinical and laboratory-negative liver failure.

Adult↗